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The reactions of glial cells and endoneurial macrophages in the dorsal root ganglion and their contribution to pain-related behavior after application of nucleus pulposus onto the nerve root in rats.
Spine (Phila Pa 1976). 2010 Feb 01; 35(3):264-71.S

Abstract

STUDY DESIGN

Controlled, interventional, animal study.

OBJECTIVE

To observe the reaction of glial cells and endoneurial macrophages in the dorsal root ganglion (DRG) after application of nucleus pulposus (NP) and investigate whether activated DRG glial cells play a role in the pathogenesis of neuropathic pain.

SUMMARY OF BACKGROUND DATA

Peripheral nerve injury activated DRG and spinal cord glial cells and several cytokines and neurotrophins released from these activated glial cells might induce pain hypersensitivity.

METHODS

Adult male Sprague-Dawley rats were used. NP harvested from the tail was applied to the left L5 DRG. Behavioral testing was performed to investigate the mechanical withdrawal threshold. The numbers of activated satellite glial cells and endoneurial macrophages were counted, and the expressions of tumor necrosis factor-alpha (TNF-alpha) and glial cell-line derived neurotrophic factor (GDNF) were examined by double-labeled immunohistochemistry and immunoblotting.

RESULTS

The mechanical withdrawal threshold was significantly decreased for 28 days and then gradually recovered (P < 0.05). Long-term activation of endoneurial macrophages and satellite glial cells in the DRG was observed, and the reactions of these cells correlated well with pain-related behavior. TNF-alpha was expressed in both endoneurial macrophages and activated satellite glial cells, and TNF-alpha expression was significantly increased in the early stage (P < 0.05). Activated satellite glial cells also expressed GDNF, and its expression was significantly increased and persisted for 28 days (P < 0.05).

CONCLUSION

Activation of DRG glial cells and endoneurial macrophages plays an important role in the pathogenesis of the neuropathic pain state. TNF-alpha actively released from activated glial cells and endoneurial macrophages in the DRG might initiate and maintain the neuropathic pain together with TNF-alpha derived from the applied NP. In the recovery phase, persistent expression of GDNF from activated satellite glial cells might play an important role to restore the function of damaged neurons and recover from neuropathic pain.

Authors+Show Affiliations

Department of Orthopaedic Surgery, Fukushima Medical University School of Medicine, Fukushima, Japan. kootoshi@fmu.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

20075775

Citation

Otoshi, Ken-ichi, et al. "The Reactions of Glial Cells and Endoneurial Macrophages in the Dorsal Root Ganglion and Their Contribution to Pain-related Behavior After Application of Nucleus Pulposus Onto the Nerve Root in Rats." Spine, vol. 35, no. 3, 2010, pp. 264-71.
Otoshi K, Kikuchi S, Konno S, et al. The reactions of glial cells and endoneurial macrophages in the dorsal root ganglion and their contribution to pain-related behavior after application of nucleus pulposus onto the nerve root in rats. Spine (Phila Pa 1976). 2010;35(3):264-71.
Otoshi, K., Kikuchi, S., Konno, S., & Sekiguchi, M. (2010). The reactions of glial cells and endoneurial macrophages in the dorsal root ganglion and their contribution to pain-related behavior after application of nucleus pulposus onto the nerve root in rats. Spine, 35(3), 264-71. https://doi.org/10.1097/BRS.0b013e3181b8b04f
Otoshi K, et al. The Reactions of Glial Cells and Endoneurial Macrophages in the Dorsal Root Ganglion and Their Contribution to Pain-related Behavior After Application of Nucleus Pulposus Onto the Nerve Root in Rats. Spine (Phila Pa 1976). 2010 Feb 1;35(3):264-71. PubMed PMID: 20075775.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The reactions of glial cells and endoneurial macrophages in the dorsal root ganglion and their contribution to pain-related behavior after application of nucleus pulposus onto the nerve root in rats. AU - Otoshi,Ken-ichi, AU - Kikuchi,Shin-ichi, AU - Konno,Shin-ichi, AU - Sekiguchi,Miho, PY - 2010/1/16/entrez PY - 2010/1/16/pubmed PY - 2010/9/14/medline SP - 264 EP - 71 JF - Spine JO - Spine (Phila Pa 1976) VL - 35 IS - 3 N2 - STUDY DESIGN: Controlled, interventional, animal study. OBJECTIVE: To observe the reaction of glial cells and endoneurial macrophages in the dorsal root ganglion (DRG) after application of nucleus pulposus (NP) and investigate whether activated DRG glial cells play a role in the pathogenesis of neuropathic pain. SUMMARY OF BACKGROUND DATA: Peripheral nerve injury activated DRG and spinal cord glial cells and several cytokines and neurotrophins released from these activated glial cells might induce pain hypersensitivity. METHODS: Adult male Sprague-Dawley rats were used. NP harvested from the tail was applied to the left L5 DRG. Behavioral testing was performed to investigate the mechanical withdrawal threshold. The numbers of activated satellite glial cells and endoneurial macrophages were counted, and the expressions of tumor necrosis factor-alpha (TNF-alpha) and glial cell-line derived neurotrophic factor (GDNF) were examined by double-labeled immunohistochemistry and immunoblotting. RESULTS: The mechanical withdrawal threshold was significantly decreased for 28 days and then gradually recovered (P < 0.05). Long-term activation of endoneurial macrophages and satellite glial cells in the DRG was observed, and the reactions of these cells correlated well with pain-related behavior. TNF-alpha was expressed in both endoneurial macrophages and activated satellite glial cells, and TNF-alpha expression was significantly increased in the early stage (P < 0.05). Activated satellite glial cells also expressed GDNF, and its expression was significantly increased and persisted for 28 days (P < 0.05). CONCLUSION: Activation of DRG glial cells and endoneurial macrophages plays an important role in the pathogenesis of the neuropathic pain state. TNF-alpha actively released from activated glial cells and endoneurial macrophages in the DRG might initiate and maintain the neuropathic pain together with TNF-alpha derived from the applied NP. In the recovery phase, persistent expression of GDNF from activated satellite glial cells might play an important role to restore the function of damaged neurons and recover from neuropathic pain. SN - 1528-1159 UR - https://www.unboundmedicine.com/medline/citation/20075775/The_reactions_of_glial_cells_and_endoneurial_macrophages_in_the_dorsal_root_ganglion_and_their_contribution_to_pain_related_behavior_after_application_of_nucleus_pulposus_onto_the_nerve_root_in_rats_ L2 - https://doi.org/10.1097/BRS.0b013e3181b8b04f DB - PRIME DP - Unbound Medicine ER -