Tags

Type your tag names separated by a space and hit enter

A prospective molecular surveillance study evaluating the clinical impact of community-acquired respiratory viruses in lung transplant recipients.
Transplantation 2010; 89(8):1028-33T

Abstract

BACKGROUND

Community-acquired respiratory viral infections (RVIs) are common in lung transplant patients and may be associated with acute rejection and bronchiolitis obliterans syndrome (BOS). The use of sensitive molecular methods that can simultaneously detect a large panel of respiratory viruses may help better define their effects.

METHODS

Lung transplant recipients undergoing serial surveillance and diagnostic bronchoalveolar lavages (BALs) during a period of 3 years were enrolled. BAL samples underwent multiplex testing for a panel of 19 respiratory viral types/subtypes using the Luminex xTAG respiratory virus panel assay.

RESULTS

Demographics, symptoms, and forced expiratory volume in 1 sec were prospectively collected for 93 lung transplant recipients enrolled. Mean number of BAL samples was 6.2+/-3.1 per patient. A respiratory virus was isolated in 48 of 93 (51.6%) patients on at least one BAL sample. Of 81 positive samples, the viruses isolated included rhinovirus (n=46), parainfluenza 1 to 4 (n=17), coronavirus (n=11), influenza (n=4), metapneumovirus (n=4), and respiratory syncytial virus (n=2). Biopsy-proven acute rejection (> or =grade 2) or decline in forced expiratory volume in 1 sec > or =20% occurred in 16 of 48 (33.3%) patients within 3 months of RVI when compared with 3 of 45 (6.7%) RVI-negative patients within a comparable time frame (P=0.001). No significant difference was seen in incidence of acute rejection between symptomatic and asymptomatic patients. Biopsy-proven obliterative bronchiolitis or BOS was diagnosed in 10 of 16 (62.5%) patients within 1 year of infection.

CONCLUSION

Community-acquired RVIs are frequently detected in BAL samples from lung transplant patients. In a significant percentage of patients, symptomatic or asymptomatic viral infection is a trigger for acute rejection and obliterative bronchiolitis/BOS.

Authors+Show Affiliations

Transplant Infectious Diseases, University of Alberta, Edmonton, Alberta, Canada. deepali.kumar@ualberta.caNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20075787

Citation

Kumar, Deepali, et al. "A Prospective Molecular Surveillance Study Evaluating the Clinical Impact of Community-acquired Respiratory Viruses in Lung Transplant Recipients." Transplantation, vol. 89, no. 8, 2010, pp. 1028-33.
Kumar D, Husain S, Chen MH, et al. A prospective molecular surveillance study evaluating the clinical impact of community-acquired respiratory viruses in lung transplant recipients. Transplantation. 2010;89(8):1028-33.
Kumar, D., Husain, S., Chen, M. H., Moussa, G., Himsworth, D., Manuel, O., ... Humar, A. (2010). A prospective molecular surveillance study evaluating the clinical impact of community-acquired respiratory viruses in lung transplant recipients. Transplantation, 89(8), pp. 1028-33. doi:10.1097/TP.0b013e3181d05a71.
Kumar D, et al. A Prospective Molecular Surveillance Study Evaluating the Clinical Impact of Community-acquired Respiratory Viruses in Lung Transplant Recipients. Transplantation. 2010 Apr 27;89(8):1028-33. PubMed PMID: 20075787.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A prospective molecular surveillance study evaluating the clinical impact of community-acquired respiratory viruses in lung transplant recipients. AU - Kumar,Deepali, AU - Husain,Shahid, AU - Chen,Maggie Hong, AU - Moussa,George, AU - Himsworth,David, AU - Manuel,Oriol, AU - Studer,Sean, AU - Pakstis,Diana, AU - McCurry,Kenneth, AU - Doucette,Karen, AU - Pilewski,Joseph, AU - Janeczko,Richard, AU - Humar,Atul, PY - 2010/1/16/entrez PY - 2010/1/16/pubmed PY - 2010/4/30/medline SP - 1028 EP - 33 JF - Transplantation JO - Transplantation VL - 89 IS - 8 N2 - BACKGROUND: Community-acquired respiratory viral infections (RVIs) are common in lung transplant patients and may be associated with acute rejection and bronchiolitis obliterans syndrome (BOS). The use of sensitive molecular methods that can simultaneously detect a large panel of respiratory viruses may help better define their effects. METHODS: Lung transplant recipients undergoing serial surveillance and diagnostic bronchoalveolar lavages (BALs) during a period of 3 years were enrolled. BAL samples underwent multiplex testing for a panel of 19 respiratory viral types/subtypes using the Luminex xTAG respiratory virus panel assay. RESULTS: Demographics, symptoms, and forced expiratory volume in 1 sec were prospectively collected for 93 lung transplant recipients enrolled. Mean number of BAL samples was 6.2+/-3.1 per patient. A respiratory virus was isolated in 48 of 93 (51.6%) patients on at least one BAL sample. Of 81 positive samples, the viruses isolated included rhinovirus (n=46), parainfluenza 1 to 4 (n=17), coronavirus (n=11), influenza (n=4), metapneumovirus (n=4), and respiratory syncytial virus (n=2). Biopsy-proven acute rejection (> or =grade 2) or decline in forced expiratory volume in 1 sec > or =20% occurred in 16 of 48 (33.3%) patients within 3 months of RVI when compared with 3 of 45 (6.7%) RVI-negative patients within a comparable time frame (P=0.001). No significant difference was seen in incidence of acute rejection between symptomatic and asymptomatic patients. Biopsy-proven obliterative bronchiolitis or BOS was diagnosed in 10 of 16 (62.5%) patients within 1 year of infection. CONCLUSION: Community-acquired RVIs are frequently detected in BAL samples from lung transplant patients. In a significant percentage of patients, symptomatic or asymptomatic viral infection is a trigger for acute rejection and obliterative bronchiolitis/BOS. SN - 1534-6080 UR - https://www.unboundmedicine.com/medline/citation/20075787/A_prospective_molecular_surveillance_study_evaluating_the_clinical_impact_of_community_acquired_respiratory_viruses_in_lung_transplant_recipients_ L2 - http://dx.doi.org/10.1097/TP.0b013e3181d05a71 DB - PRIME DP - Unbound Medicine ER -