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Quantitation of argatroban in plasma using liquid chromatography electrospray tandem mass spectrometry (UPLC-ESI-MS/MS).
Methods Mol Biol. 2010; 603:57-63.MM

Abstract

The following chapter describes a method to measure argatroban in plasma samples using ultra-performance liquid chromatography combined with electrospray positive ionization tandem mass spectrometry (UPLC-ESI-MS/MS). Samples are pre-treated using methanol containing the internal standard (IS) diclofenac. The plasma extracts are dried under a stream of nitrogen. The residue is reconstituted in ammonium acetate-formic acid-water. The reconstituted plasma is injected into the UPLC-ESI-MS/MS. Argatroban and diclofenac show similar retention times in plasma. Quantification of argatroban in the samples is made by multiple reaction monitoring using the hydrogen adduct mass transitions, from a seven-point calibration curve.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Molinaro RJ
Department of Pathology and Laboratory Medicine, Emory University School of Medicine and Emory Healthcare Systems, Atlanta, GA, USA.

MeSH

ArginineChromatography, LiquidHumansPipecolic AcidsReproducibility of ResultsSpectrometry, Mass, Electrospray IonizationSulfonamidesTandem Mass Spectrometry

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20077059

Citation

Molinaro, Ross J.. "Quantitation of Argatroban in Plasma Using Liquid Chromatography Electrospray Tandem Mass Spectrometry (UPLC-ESI-MS/MS)." Methods in Molecular Biology (Clifton, N.J.), vol. 603, 2010, pp. 57-63.
Molinaro RJ. Quantitation of argatroban in plasma using liquid chromatography electrospray tandem mass spectrometry (UPLC-ESI-MS/MS). Methods Mol Biol. 2010;603:57-63.
Molinaro, R. J. (2010). Quantitation of argatroban in plasma using liquid chromatography electrospray tandem mass spectrometry (UPLC-ESI-MS/MS). Methods in Molecular Biology (Clifton, N.J.), 603, 57-63. https://doi.org/10.1007/978-1-60761-459-3_6
Molinaro RJ. Quantitation of Argatroban in Plasma Using Liquid Chromatography Electrospray Tandem Mass Spectrometry (UPLC-ESI-MS/MS). Methods Mol Biol. 2010;603:57-63. PubMed PMID: 20077059.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitation of argatroban in plasma using liquid chromatography electrospray tandem mass spectrometry (UPLC-ESI-MS/MS). A1 - Molinaro,Ross J, PY - 2010/1/16/entrez PY - 2010/1/16/pubmed PY - 2010/4/7/medline SP - 57 EP - 63 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol Biol VL - 603 N2 - The following chapter describes a method to measure argatroban in plasma samples using ultra-performance liquid chromatography combined with electrospray positive ionization tandem mass spectrometry (UPLC-ESI-MS/MS). Samples are pre-treated using methanol containing the internal standard (IS) diclofenac. The plasma extracts are dried under a stream of nitrogen. The residue is reconstituted in ammonium acetate-formic acid-water. The reconstituted plasma is injected into the UPLC-ESI-MS/MS. Argatroban and diclofenac show similar retention times in plasma. Quantification of argatroban in the samples is made by multiple reaction monitoring using the hydrogen adduct mass transitions, from a seven-point calibration curve. SN - 1940-6029 UR - https://www.unboundmedicine.com/medline/citation/20077059/Quantitation_of_argatroban_in_plasma_using_liquid_chromatography_electrospray_tandem_mass_spectrometry__UPLC_ESI_MS/MS__ L2 - https://dx.doi.org/10.1007/978-1-60761-459-3_6 DB - PRIME DP - Unbound Medicine ER -