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Quantitation of total 11-nor-9-carboxy-delta 9-tetrahydrocannabinol in urine and blood using gas chromatography-mass spectrometry (GC-MS).
Methods Mol Biol. 2010; 603:137-44.MM

Abstract

Marijuana, which is made from crushing the leaves, flowers, and sometimes the stems of the plant Cannabis sativa, contains more than 30 cannabinoids. The major psychoactive cannabinoid is delta-9-tetrahydrocannabinol (THC). The major metabolite of THC, 11-nor-delta 9-carboxy-tetrahydrocannabionol (THC-COOH), is excreted in the urine primarily as a glucuronide conjugate and is commonly analyzed in biological specimens for detecting marijuana usage. The procedure described here involves the addition of deuterated internal standard THC-COOH-d9 into the sample followed by hydrolysis of conjugated THC-COOH by alkali. THC-COOH is extracted from urine or blood using liquid-liquid extraction followed by preparation of its trimethylsilyl derivatives. The analysis of derivatized THC-COOH is performed using gas-chromatography/mass spectrometry (GC/MS). Quantification of the drug in a sample is achieved by comparing the responses of the unknown sample to the responses of the calibrators using selected ion monitoring.

Authors+Show Affiliations

Department of Pathology and Laboratory Medicine, Children's Mercy Hospitals and Clinics, Kansas City, MO, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20077066

Citation

Frazee, C Clinton, et al. "Quantitation of Total 11-nor-9-carboxy-delta 9-tetrahydrocannabinol in Urine and Blood Using Gas Chromatography-mass Spectrometry (GC-MS)." Methods in Molecular Biology (Clifton, N.J.), vol. 603, 2010, pp. 137-44.
Frazee CC, Kiscoan M, Garg U. Quantitation of total 11-nor-9-carboxy-delta 9-tetrahydrocannabinol in urine and blood using gas chromatography-mass spectrometry (GC-MS). Methods Mol Biol. 2010;603:137-44.
Frazee, C. C., Kiscoan, M., & Garg, U. (2010). Quantitation of total 11-nor-9-carboxy-delta 9-tetrahydrocannabinol in urine and blood using gas chromatography-mass spectrometry (GC-MS). Methods in Molecular Biology (Clifton, N.J.), 603, 137-44. https://doi.org/10.1007/978-1-60761-459-3_13
Frazee CC, Kiscoan M, Garg U. Quantitation of Total 11-nor-9-carboxy-delta 9-tetrahydrocannabinol in Urine and Blood Using Gas Chromatography-mass Spectrometry (GC-MS). Methods Mol Biol. 2010;603:137-44. PubMed PMID: 20077066.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitation of total 11-nor-9-carboxy-delta 9-tetrahydrocannabinol in urine and blood using gas chromatography-mass spectrometry (GC-MS). AU - Frazee,C Clinton,3rd AU - Kiscoan,Michael, AU - Garg,Uttam, PY - 2010/1/16/entrez PY - 2010/1/16/pubmed PY - 2010/4/7/medline SP - 137 EP - 44 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol Biol VL - 603 N2 - Marijuana, which is made from crushing the leaves, flowers, and sometimes the stems of the plant Cannabis sativa, contains more than 30 cannabinoids. The major psychoactive cannabinoid is delta-9-tetrahydrocannabinol (THC). The major metabolite of THC, 11-nor-delta 9-carboxy-tetrahydrocannabionol (THC-COOH), is excreted in the urine primarily as a glucuronide conjugate and is commonly analyzed in biological specimens for detecting marijuana usage. The procedure described here involves the addition of deuterated internal standard THC-COOH-d9 into the sample followed by hydrolysis of conjugated THC-COOH by alkali. THC-COOH is extracted from urine or blood using liquid-liquid extraction followed by preparation of its trimethylsilyl derivatives. The analysis of derivatized THC-COOH is performed using gas-chromatography/mass spectrometry (GC/MS). Quantification of the drug in a sample is achieved by comparing the responses of the unknown sample to the responses of the calibrators using selected ion monitoring. SN - 1940-6029 UR - https://www.unboundmedicine.com/medline/citation/20077066/Quantitation_of_total_11_nor_9_carboxy_delta_9_tetrahydrocannabinol_in_urine_and_blood_using_gas_chromatography_mass_spectrometry__GC_MS__ L2 - https://dx.doi.org/10.1007/978-1-60761-459-3_13 DB - PRIME DP - Unbound Medicine ER -