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Quantitation of cocaine, benzoylecgonine, ecgonine methyl ester, and cocaethylene in urine and blood using gas chromatography-mass spectrometry (GC-MS).
Methods Mol Biol. 2010; 603:145-56.MM

Abstract

Cocaine, a stimulant, is a commonly abused drug. Cocaine and its metabolites are measured in various biological specimens for clinical and forensic purposes. Urine or plasma or serum is spiked with deuterated internal standards cocaine-d3, benzoylecgonine-d3, ecgonine methyl ester-d3, and cocaethylene-d3 and buffered with phosphate buffer. The drugs in the sample are extracted by cation-exchange solid phase extraction. The drugs from the solid phase cartridge are eluted and the eluent is dried under the stream of nitrogen. The residue is incubated with pentafluoropropionic acid anhydride and pentafluoropropanol to form pentafluoropropionyl derivatives of ecgonine methyl ester and benzoylecgonine. Cocaine and cocaethylene are refractory to derivatization. The extract is dried, reconstituted in ethyl acetate, and injected into gas chromatography mass-spectrometry analyzer. Quantitation of the drugs in the samples is made, using selected ion monitoring, from a 3-point calibration curve.

Authors+Show Affiliations

Department of Pathology and Laboratory Medicine, Children's Mercy Hospitals and Clinics, Kansas City, MO, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20077067

Citation

Fleming, Steven W., et al. "Quantitation of Cocaine, Benzoylecgonine, Ecgonine Methyl Ester, and Cocaethylene in Urine and Blood Using Gas Chromatography-mass Spectrometry (GC-MS)." Methods in Molecular Biology (Clifton, N.J.), vol. 603, 2010, pp. 145-56.
Fleming SW, Dasgupta A, Garg U. Quantitation of cocaine, benzoylecgonine, ecgonine methyl ester, and cocaethylene in urine and blood using gas chromatography-mass spectrometry (GC-MS). Methods Mol Biol. 2010;603:145-56.
Fleming, S. W., Dasgupta, A., & Garg, U. (2010). Quantitation of cocaine, benzoylecgonine, ecgonine methyl ester, and cocaethylene in urine and blood using gas chromatography-mass spectrometry (GC-MS). Methods in Molecular Biology (Clifton, N.J.), 603, 145-56. https://doi.org/10.1007/978-1-60761-459-3_14
Fleming SW, Dasgupta A, Garg U. Quantitation of Cocaine, Benzoylecgonine, Ecgonine Methyl Ester, and Cocaethylene in Urine and Blood Using Gas Chromatography-mass Spectrometry (GC-MS). Methods Mol Biol. 2010;603:145-56. PubMed PMID: 20077067.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitation of cocaine, benzoylecgonine, ecgonine methyl ester, and cocaethylene in urine and blood using gas chromatography-mass spectrometry (GC-MS). AU - Fleming,Steven W, AU - Dasgupta,Amitava, AU - Garg,Uttam, PY - 2010/1/16/entrez PY - 2010/1/16/pubmed PY - 2010/4/7/medline SP - 145 EP - 56 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol Biol VL - 603 N2 - Cocaine, a stimulant, is a commonly abused drug. Cocaine and its metabolites are measured in various biological specimens for clinical and forensic purposes. Urine or plasma or serum is spiked with deuterated internal standards cocaine-d3, benzoylecgonine-d3, ecgonine methyl ester-d3, and cocaethylene-d3 and buffered with phosphate buffer. The drugs in the sample are extracted by cation-exchange solid phase extraction. The drugs from the solid phase cartridge are eluted and the eluent is dried under the stream of nitrogen. The residue is incubated with pentafluoropropionic acid anhydride and pentafluoropropanol to form pentafluoropropionyl derivatives of ecgonine methyl ester and benzoylecgonine. Cocaine and cocaethylene are refractory to derivatization. The extract is dried, reconstituted in ethyl acetate, and injected into gas chromatography mass-spectrometry analyzer. Quantitation of the drugs in the samples is made, using selected ion monitoring, from a 3-point calibration curve. SN - 1940-6029 UR - https://www.unboundmedicine.com/medline/citation/20077067/Quantitation_of_cocaine_benzoylecgonine_ecgonine_methyl_ester_and_cocaethylene_in_urine_and_blood_using_gas_chromatography_mass_spectrometry__GC_MS__ L2 - https://dx.doi.org/10.1007/978-1-60761-459-3_14 DB - PRIME DP - Unbound Medicine ER -