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Quantitation of nicotine, its metabolites, and other related alkaloids in urine, serum, and plasma using LC-MS-MS.
Methods Mol Biol. 2010; 603:389-98.MM

Abstract

We describe a method for the quantitative analysis of nicotine, cotinine, trans-3'-hydroxy cotinine, nornicotine, and anabasine in urine, serum, and plasma using liquid chromatography-tandem mass spectrometry. A mix of deuterium-labeled internal standards (IS) is added to a specimen aliquot. The aliquot is extracted using mixed-mode solid phase extraction and eluted into an autosampler vial for injection into an LC-MS-MS system. An Atlantis silica column is used for LC separation in hydrophilic interaction mode. Tandem mass spectrometry detection is performed in positive ion mode with electrospray ionization and two multiple reaction monitoring (MRM) transitions monitored for each analyte and IS.

Authors+Show Affiliations

ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20077091

Citation

Yue, Bingfang, et al. "Quantitation of Nicotine, Its Metabolites, and Other Related Alkaloids in Urine, Serum, and Plasma Using LC-MS-MS." Methods in Molecular Biology (Clifton, N.J.), vol. 603, 2010, pp. 389-98.
Yue B, Kushnir MM, Urry FM, et al. Quantitation of nicotine, its metabolites, and other related alkaloids in urine, serum, and plasma using LC-MS-MS. Methods Mol Biol. 2010;603:389-98.
Yue, B., Kushnir, M. M., Urry, F. M., & Rockwood, A. L. (2010). Quantitation of nicotine, its metabolites, and other related alkaloids in urine, serum, and plasma using LC-MS-MS. Methods in Molecular Biology (Clifton, N.J.), 603, 389-98. https://doi.org/10.1007/978-1-60761-459-3_38
Yue B, et al. Quantitation of Nicotine, Its Metabolites, and Other Related Alkaloids in Urine, Serum, and Plasma Using LC-MS-MS. Methods Mol Biol. 2010;603:389-98. PubMed PMID: 20077091.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitation of nicotine, its metabolites, and other related alkaloids in urine, serum, and plasma using LC-MS-MS. AU - Yue,Bingfang, AU - Kushnir,Mark M, AU - Urry,Francis M, AU - Rockwood,Alan L, PY - 2010/1/16/entrez PY - 2010/1/16/pubmed PY - 2010/4/7/medline SP - 389 EP - 98 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol Biol VL - 603 N2 - We describe a method for the quantitative analysis of nicotine, cotinine, trans-3'-hydroxy cotinine, nornicotine, and anabasine in urine, serum, and plasma using liquid chromatography-tandem mass spectrometry. A mix of deuterium-labeled internal standards (IS) is added to a specimen aliquot. The aliquot is extracted using mixed-mode solid phase extraction and eluted into an autosampler vial for injection into an LC-MS-MS system. An Atlantis silica column is used for LC separation in hydrophilic interaction mode. Tandem mass spectrometry detection is performed in positive ion mode with electrospray ionization and two multiple reaction monitoring (MRM) transitions monitored for each analyte and IS. SN - 1940-6029 UR - https://www.unboundmedicine.com/medline/citation/20077091/Quantitation_of_nicotine_its_metabolites_and_other_related_alkaloids_in_urine_serum_and_plasma_using_LC_MS_MS_ L2 - https://dx.doi.org/10.1007/978-1-60761-459-3_38 DB - PRIME DP - Unbound Medicine ER -