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Quantitation of oxycodone in blood and urine using gas chromatography-mass spectrometry (GC-MS).
Methods Mol Biol. 2010; 603:453-60.MM

Abstract

Oxycodone is a semisynthetic opioid analgesic used in pain management. In the following method, gas chromatography-mass spectrometry (GC-MS) is used to determine the presence and concentration of the drug in blood and urine. The specimens are fortified with deuterated internal standard and a five-point calibration curve is constructed. Specimens are extracted using mixed-mode solid phase extraction and derivatized with N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) producing trimethylsilyl derivatives. The final extracts are then analyzed using selected ion monitoring GC-MS.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Goldberger BA
Department of Pathology, Immunology, and Laboratory Medicine, University of Florida College of Medicine, Gainesville, FL, USA.
Chronister CW
No affiliation info available
Merves ML
No affiliation info available

MeSH

Gas Chromatography-Mass SpectrometryHumansOxycodoneReproducibility of ResultsSolid Phase ExtractionTrimethylsilyl Compounds

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20077097

Citation

Goldberger, Bruce A., et al. "Quantitation of Oxycodone in Blood and Urine Using Gas Chromatography-mass Spectrometry (GC-MS)." Methods in Molecular Biology (Clifton, N.J.), vol. 603, 2010, pp. 453-60.
Goldberger BA, Chronister CW, Merves ML. Quantitation of oxycodone in blood and urine using gas chromatography-mass spectrometry (GC-MS). Methods Mol Biol. 2010;603:453-60.
Goldberger, B. A., Chronister, C. W., & Merves, M. L. (2010). Quantitation of oxycodone in blood and urine using gas chromatography-mass spectrometry (GC-MS). Methods in Molecular Biology (Clifton, N.J.), 603, 453-60. https://doi.org/10.1007/978-1-60761-459-3_44
Goldberger BA, Chronister CW, Merves ML. Quantitation of Oxycodone in Blood and Urine Using Gas Chromatography-mass Spectrometry (GC-MS). Methods Mol Biol. 2010;603:453-60. PubMed PMID: 20077097.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitation of oxycodone in blood and urine using gas chromatography-mass spectrometry (GC-MS). AU - Goldberger,Bruce A, AU - Chronister,Chris W, AU - Merves,Michele L, PY - 2010/1/16/entrez PY - 2010/1/16/pubmed PY - 2010/4/7/medline SP - 453 EP - 60 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol Biol VL - 603 N2 - Oxycodone is a semisynthetic opioid analgesic used in pain management. In the following method, gas chromatography-mass spectrometry (GC-MS) is used to determine the presence and concentration of the drug in blood and urine. The specimens are fortified with deuterated internal standard and a five-point calibration curve is constructed. Specimens are extracted using mixed-mode solid phase extraction and derivatized with N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) producing trimethylsilyl derivatives. The final extracts are then analyzed using selected ion monitoring GC-MS. SN - 1940-6029 UR - https://www.unboundmedicine.com/medline/citation/20077097/Quantitation_of_oxycodone_in_blood_and_urine_using_gas_chromatography_mass_spectrometry__GC_MS__ L2 - https://dx.doi.org/10.1007/978-1-60761-459-3_44 DB - PRIME DP - Unbound Medicine ER -