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Quantitation of phencyclidine (PCP) in urine and blood using gas chromatography-mass spectrometry (GC-MS).
Methods Mol Biol. 2010; 603:461-7.MM

Abstract

Phencyclidine (PCP) is a cycloalkylamine and is classified as a dissociative anesthetic. In the 1950s, PCP was tested as an intravenous anesthetic but due to its severe side effects, it was withdrawn from the clinical use. Since then PCP has become an illegal street drug making its laboratory analysis forensically essential. PCP can be detected in urine, serum, or plasma by immunoassays and quantified by gas or liquid chromatography mass spectrometry. In the method described here, a deuterated internal standard is added to the sample and the drug is extracted under alkaline conditions. Analysis is conducted using gas chromatography mass spectrometry (GC-MS). Quantitation of PCP is done by comparing the responses of unknown samples to the standards using selected ion monitoring.

Authors+Show Affiliations

Department of Pathology and Laboratory Medicine, Children's Mercy Hospitals and Clinics, Kansas City, MO, USA.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20077098

Citation

Ferguson, Angela M., and Uttam Garg. "Quantitation of Phencyclidine (PCP) in Urine and Blood Using Gas Chromatography-mass Spectrometry (GC-MS)." Methods in Molecular Biology (Clifton, N.J.), vol. 603, 2010, pp. 461-7.
Ferguson AM, Garg U. Quantitation of phencyclidine (PCP) in urine and blood using gas chromatography-mass spectrometry (GC-MS). Methods Mol Biol. 2010;603:461-7.
Ferguson, A. M., & Garg, U. (2010). Quantitation of phencyclidine (PCP) in urine and blood using gas chromatography-mass spectrometry (GC-MS). Methods in Molecular Biology (Clifton, N.J.), 603, 461-7. https://doi.org/10.1007/978-1-60761-459-3_45
Ferguson AM, Garg U. Quantitation of Phencyclidine (PCP) in Urine and Blood Using Gas Chromatography-mass Spectrometry (GC-MS). Methods Mol Biol. 2010;603:461-7. PubMed PMID: 20077098.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitation of phencyclidine (PCP) in urine and blood using gas chromatography-mass spectrometry (GC-MS). AU - Ferguson,Angela M, AU - Garg,Uttam, PY - 2010/1/16/entrez PY - 2010/1/16/pubmed PY - 2010/4/7/medline SP - 461 EP - 7 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol Biol VL - 603 N2 - Phencyclidine (PCP) is a cycloalkylamine and is classified as a dissociative anesthetic. In the 1950s, PCP was tested as an intravenous anesthetic but due to its severe side effects, it was withdrawn from the clinical use. Since then PCP has become an illegal street drug making its laboratory analysis forensically essential. PCP can be detected in urine, serum, or plasma by immunoassays and quantified by gas or liquid chromatography mass spectrometry. In the method described here, a deuterated internal standard is added to the sample and the drug is extracted under alkaline conditions. Analysis is conducted using gas chromatography mass spectrometry (GC-MS). Quantitation of PCP is done by comparing the responses of unknown samples to the standards using selected ion monitoring. SN - 1940-6029 UR - https://www.unboundmedicine.com/medline/citation/20077098/Quantitation_of_phencyclidine__PCP__in_urine_and_blood_using_gas_chromatography_mass_spectrometry__GC_MS__ L2 - https://dx.doi.org/10.1007/978-1-60761-459-3_45 DB - PRIME DP - Unbound Medicine ER -