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Bioavailability and catabolism of green tea flavan-3-ols in humans.
Nutrition. 2010 Nov-Dec; 26(11-12):1110-6.N

Abstract

OBJECTIVE

The aim of this study was to investigate green tea flavan-3-ol catabolism and plasma pharmacokinetic and urinary excretion by high-performance liquid chromatography with tandem mass spectrometry to evaluate their absolute bioavailability by taking into account all known and some unknown catabolites deriving from their interaction with the gastrointestinal tract and its host microflora.

METHODS

A feeding study was carried out in 20 healthy human volunteers who ingested 400 mL of a ready-to-drink green tea containing approximately 400 μmol of flavan-3-ols. Urine and plasma were collected for 4 and 24h, respectively, and 39 relevant catabolites were identified in these biological fluids by tandem mass spectrometry.

RESULTS

In biological fluids, 39 relevant flavan-3-ol catabolites were identified. In plasma, (-)-epigallocatechin-3-gallate was the only unmetabolized compound and the highest in absolute concentration compared with (-)-epigallocatechin and (-)-epicatechin conjugates. Colonic microflora-derived polyhydroxyphenyl-γ-valerolactones were by far the main urinary catabolites, averaging 10 times greater concentratin than flavan-3-ol conjugates. The calculated bioavailability was equal to 39% and it is interesting to notice the great variability in urinary excretion of colonic metabolites among participants, probably related to differences in their own colonic microflora.

CONCLUSION

This study demonstrates that green tea catechins are more bioavailable than previously observed when colonic ring fission metabolites are taken into consideration. Regular consumption of ready-to-drink green tea containing flavan-3-ols allows a non-marginal exposure of the human body to these catabolites, somehow justifying the numerous beneficial actions described as linked to green tea intake.

Authors+Show Affiliations

Department of Public Health, University of Parma, Parma, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20080030

Citation

Del Rio, Daniele, et al. "Bioavailability and Catabolism of Green Tea Flavan-3-ols in Humans." Nutrition (Burbank, Los Angeles County, Calif.), vol. 26, no. 11-12, 2010, pp. 1110-6.
Del Rio D, Calani L, Cordero C, et al. Bioavailability and catabolism of green tea flavan-3-ols in humans. Nutrition. 2010;26(11-12):1110-6.
Del Rio, D., Calani, L., Cordero, C., Salvatore, S., Pellegrini, N., & Brighenti, F. (2010). Bioavailability and catabolism of green tea flavan-3-ols in humans. Nutrition (Burbank, Los Angeles County, Calif.), 26(11-12), 1110-6. https://doi.org/10.1016/j.nut.2009.09.021
Del Rio D, et al. Bioavailability and Catabolism of Green Tea Flavan-3-ols in Humans. Nutrition. 2010 Nov-Dec;26(11-12):1110-6. PubMed PMID: 20080030.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bioavailability and catabolism of green tea flavan-3-ols in humans. AU - Del Rio,Daniele, AU - Calani,Luca, AU - Cordero,Chiara, AU - Salvatore,Sara, AU - Pellegrini,Nicoletta, AU - Brighenti,Furio, Y1 - 2010/01/15/ PY - 2009/04/30/received PY - 2009/09/09/revised PY - 2009/09/17/accepted PY - 2010/1/19/entrez PY - 2010/1/19/pubmed PY - 2011/2/23/medline SP - 1110 EP - 6 JF - Nutrition (Burbank, Los Angeles County, Calif.) JO - Nutrition VL - 26 IS - 11-12 N2 - OBJECTIVE: The aim of this study was to investigate green tea flavan-3-ol catabolism and plasma pharmacokinetic and urinary excretion by high-performance liquid chromatography with tandem mass spectrometry to evaluate their absolute bioavailability by taking into account all known and some unknown catabolites deriving from their interaction with the gastrointestinal tract and its host microflora. METHODS: A feeding study was carried out in 20 healthy human volunteers who ingested 400 mL of a ready-to-drink green tea containing approximately 400 μmol of flavan-3-ols. Urine and plasma were collected for 4 and 24h, respectively, and 39 relevant catabolites were identified in these biological fluids by tandem mass spectrometry. RESULTS: In biological fluids, 39 relevant flavan-3-ol catabolites were identified. In plasma, (-)-epigallocatechin-3-gallate was the only unmetabolized compound and the highest in absolute concentration compared with (-)-epigallocatechin and (-)-epicatechin conjugates. Colonic microflora-derived polyhydroxyphenyl-γ-valerolactones were by far the main urinary catabolites, averaging 10 times greater concentratin than flavan-3-ol conjugates. The calculated bioavailability was equal to 39% and it is interesting to notice the great variability in urinary excretion of colonic metabolites among participants, probably related to differences in their own colonic microflora. CONCLUSION: This study demonstrates that green tea catechins are more bioavailable than previously observed when colonic ring fission metabolites are taken into consideration. Regular consumption of ready-to-drink green tea containing flavan-3-ols allows a non-marginal exposure of the human body to these catabolites, somehow justifying the numerous beneficial actions described as linked to green tea intake. SN - 1873-1244 UR - https://www.unboundmedicine.com/medline/citation/20080030/Bioavailability_and_catabolism_of_green_tea_flavan_3_ols_in_humans_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0899-9007(09)00413-4 DB - PRIME DP - Unbound Medicine ER -