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Buprenorphine detection in hair samples by immunometric screening test: preliminary experience.
Forensic Sci Int. 2010 Mar 20; 196(1-3):118-20.FS

Abstract

The recent introduction of buprenorphine use by the Drug Addiction Services has induced toxicology laboratories to develop new qualitative or semiquantitative screening assay for its determination in hair samples. The aim of this preliminary study was to verify the correlation between the buprenorphine intake and the immunometric screening test results (VMA-T Comedical and buprenorphine CEDIA/Thermo-Fisher/Microgenics reagents) and therefore their comparison with the liquid chromatography coupled with mass spectrometry (LC/MS) results. Hair samples were obtained from 32 subjects without buprenorphine-therapy reported and 17 in treatment. In glass test tube with hermetic cap were weighed 33 mg of 49 finely cut hair samples, washed with 1 mL of SLV-VMA-T washing solution, which is then completely sucked and eliminated. The samples were extracted with 400 microL of VMA-T reagent for an hour at 100 degrees C. The extracts were analysed by immunometric screening test on ILab 650 chemistry analyser, using buprenorphine CEDIA reagent assay. From the 32 non-takers of drug, 30 semiquantitative results were less than 10 pg/mg and 2 were over 10 pg/mg; from the 17 subjects with therapy, all were over 10 pg/mg (range 13-50 pg/mg); no samples were false-negative. Results suggest that exist a good relationship between the administration of buprenorphine and its concentration in hair, detectable through this method and reagents line.

Authors+Show Affiliations

Laboratorio Sanità Pubblica--Azienda Provinciale per i Servizi Sanitari, Trento, Italy. fiorenza.svaizer@apss.tn.itNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20080369

Citation

Svaizer, Fiorenza, et al. "Buprenorphine Detection in Hair Samples By Immunometric Screening Test: Preliminary Experience." Forensic Science International, vol. 196, no. 1-3, 2010, pp. 118-20.
Svaizer F, Lotti A, Gottardi M, et al. Buprenorphine detection in hair samples by immunometric screening test: preliminary experience. Forensic Sci Int. 2010;196(1-3):118-20.
Svaizer, F., Lotti, A., Gottardi, M., & Miozzo, M. P. (2010). Buprenorphine detection in hair samples by immunometric screening test: preliminary experience. Forensic Science International, 196(1-3), 118-20. https://doi.org/10.1016/j.forsciint.2009.12.047
Svaizer F, et al. Buprenorphine Detection in Hair Samples By Immunometric Screening Test: Preliminary Experience. Forensic Sci Int. 2010 Mar 20;196(1-3):118-20. PubMed PMID: 20080369.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Buprenorphine detection in hair samples by immunometric screening test: preliminary experience. AU - Svaizer,Fiorenza, AU - Lotti,Andrea, AU - Gottardi,Massimo, AU - Miozzo,Maria Pia, Y1 - 2010/01/18/ PY - 2009/06/12/received PY - 2009/08/24/accepted PY - 2010/1/19/entrez PY - 2010/1/19/pubmed PY - 2010/6/11/medline SP - 118 EP - 20 JF - Forensic science international JO - Forensic Sci Int VL - 196 IS - 1-3 N2 - The recent introduction of buprenorphine use by the Drug Addiction Services has induced toxicology laboratories to develop new qualitative or semiquantitative screening assay for its determination in hair samples. The aim of this preliminary study was to verify the correlation between the buprenorphine intake and the immunometric screening test results (VMA-T Comedical and buprenorphine CEDIA/Thermo-Fisher/Microgenics reagents) and therefore their comparison with the liquid chromatography coupled with mass spectrometry (LC/MS) results. Hair samples were obtained from 32 subjects without buprenorphine-therapy reported and 17 in treatment. In glass test tube with hermetic cap were weighed 33 mg of 49 finely cut hair samples, washed with 1 mL of SLV-VMA-T washing solution, which is then completely sucked and eliminated. The samples were extracted with 400 microL of VMA-T reagent for an hour at 100 degrees C. The extracts were analysed by immunometric screening test on ILab 650 chemistry analyser, using buprenorphine CEDIA reagent assay. From the 32 non-takers of drug, 30 semiquantitative results were less than 10 pg/mg and 2 were over 10 pg/mg; from the 17 subjects with therapy, all were over 10 pg/mg (range 13-50 pg/mg); no samples were false-negative. Results suggest that exist a good relationship between the administration of buprenorphine and its concentration in hair, detectable through this method and reagents line. SN - 1872-6283 UR - https://www.unboundmedicine.com/medline/citation/20080369/Buprenorphine_detection_in_hair_samples_by_immunometric_screening_test:_preliminary_experience_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0379-0738(09)00548-9 DB - PRIME DP - Unbound Medicine ER -