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H2O2 and cytosolic Ca2+ signals triggered by the PM H-coupled transport system mediate K+/Na+ homeostasis in NaCl-stressed Populus euphratica cells.
Plant Cell Environ. 2010 Jun; 33(6):943-58.PC

Abstract

Using confocal microscopy, X-ray microanalysis and the scanning ion-selective electrode technique, we investigated the signalling of H(2)O(2), cytosolic Ca(2+) ([Ca(2+)](cyt)) and the PM H(+)-coupled transport system in K(+)/Na(+) homeostasis control in NaCl-stressed calluses of Populus euphratica. An obvious Na(+)/H(+) antiport was seen in salinized cells; however, NaCl stress caused a net K(+) efflux, because of the salt-induced membrane depolarization. H(2)O(2) levels, regulated upwards by salinity, contributed to ionic homeostasis, because H(2)O(2) restrictions by DPI or DMTU caused enhanced K(+) efflux and decreased Na(+)/H(+) antiport activity. NaCl induced a net Ca(2+) influx and a subsequent rise of [Ca(2+)](cyt), which is involved in H(2)O(2)-mediated K(+)/Na(+) homeostasis in salinized P. euphratica cells. When callus cells were pretreated with inhibitors of the Na(+)/H(+) antiport system, the NaCl-induced elevation of H(2)O(2) and [Ca(2+)](cyt) was correspondingly restricted, leading to a greater K(+) efflux and a more pronounced reduction in Na(+)/H(+) antiport activity. Results suggest that the PM H(+)-coupled transport system mediates H(+) translocation and triggers the stress signalling of H(2)O(2) and Ca(2+), which results in a K(+)/Na(+) homeostasis via mediations of K(+) channels and the Na(+)/H(+) antiport system in the PM of NaCl-stressed cells. Accordingly, a salt stress signalling pathway of P. euphratica cells is proposed.

Authors+Show Affiliations

College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20082667

Citation

Sun, Jian, et al. "H2O2 and Cytosolic Ca2+ Signals Triggered By the PM H-coupled Transport System Mediate K+/Na+ Homeostasis in NaCl-stressed Populus Euphratica Cells." Plant, Cell & Environment, vol. 33, no. 6, 2010, pp. 943-58.
Sun J, Wang MJ, Ding MQ, et al. H2O2 and cytosolic Ca2+ signals triggered by the PM H-coupled transport system mediate K+/Na+ homeostasis in NaCl-stressed Populus euphratica cells. Plant Cell Environ. 2010;33(6):943-58.
Sun, J., Wang, M. J., Ding, M. Q., Deng, S. R., Liu, M. Q., Lu, C. F., Zhou, X. Y., Shen, X., Zheng, X. J., Zhang, Z. K., Song, J., Hu, Z. M., Xu, Y., & Chen, S. L. (2010). H2O2 and cytosolic Ca2+ signals triggered by the PM H-coupled transport system mediate K+/Na+ homeostasis in NaCl-stressed Populus euphratica cells. Plant, Cell & Environment, 33(6), 943-58. https://doi.org/10.1111/j.1365-3040.2010.02118.x
Sun J, et al. H2O2 and Cytosolic Ca2+ Signals Triggered By the PM H-coupled Transport System Mediate K+/Na+ Homeostasis in NaCl-stressed Populus Euphratica Cells. Plant Cell Environ. 2010;33(6):943-58. PubMed PMID: 20082667.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - H2O2 and cytosolic Ca2+ signals triggered by the PM H-coupled transport system mediate K+/Na+ homeostasis in NaCl-stressed Populus euphratica cells. AU - Sun,Jian, AU - Wang,Mei-Juan, AU - Ding,Ming-Quan, AU - Deng,Shu-Rong, AU - Liu,Mei-Qin, AU - Lu,Cun-Fu, AU - Zhou,Xiao-Yang, AU - Shen,Xin, AU - Zheng,Xiao-Jiang, AU - Zhang,Zeng-Kai, AU - Song,Jin, AU - Hu,Zan-Min, AU - Xu,Yue, AU - Chen,Shao-Liang, Y1 - 2010/01/15/ PY - 2010/1/20/entrez PY - 2010/1/20/pubmed PY - 2010/10/13/medline SP - 943 EP - 58 JF - Plant, cell & environment JO - Plant Cell Environ VL - 33 IS - 6 N2 - Using confocal microscopy, X-ray microanalysis and the scanning ion-selective electrode technique, we investigated the signalling of H(2)O(2), cytosolic Ca(2+) ([Ca(2+)](cyt)) and the PM H(+)-coupled transport system in K(+)/Na(+) homeostasis control in NaCl-stressed calluses of Populus euphratica. An obvious Na(+)/H(+) antiport was seen in salinized cells; however, NaCl stress caused a net K(+) efflux, because of the salt-induced membrane depolarization. H(2)O(2) levels, regulated upwards by salinity, contributed to ionic homeostasis, because H(2)O(2) restrictions by DPI or DMTU caused enhanced K(+) efflux and decreased Na(+)/H(+) antiport activity. NaCl induced a net Ca(2+) influx and a subsequent rise of [Ca(2+)](cyt), which is involved in H(2)O(2)-mediated K(+)/Na(+) homeostasis in salinized P. euphratica cells. When callus cells were pretreated with inhibitors of the Na(+)/H(+) antiport system, the NaCl-induced elevation of H(2)O(2) and [Ca(2+)](cyt) was correspondingly restricted, leading to a greater K(+) efflux and a more pronounced reduction in Na(+)/H(+) antiport activity. Results suggest that the PM H(+)-coupled transport system mediates H(+) translocation and triggers the stress signalling of H(2)O(2) and Ca(2+), which results in a K(+)/Na(+) homeostasis via mediations of K(+) channels and the Na(+)/H(+) antiport system in the PM of NaCl-stressed cells. Accordingly, a salt stress signalling pathway of P. euphratica cells is proposed. SN - 1365-3040 UR - https://www.unboundmedicine.com/medline/citation/20082667/H2O2_and_cytosolic_Ca2+_signals_triggered_by_the_PM_H_coupled_transport_system_mediate_K+/Na+_homeostasis_in_NaCl_stressed_Populus_euphratica_cells_ L2 - https://doi.org/10.1111/j.1365-3040.2010.02118.x DB - PRIME DP - Unbound Medicine ER -