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[Comparison and evaluation of enzyme-linked immunization assay kits with plague reduction neutralization test for detection of measles IgG antibody].
Zhongguo Yi Miao He Mian Yi. 2009 Jun; 15(3):215-8.ZY

Abstract

OBJECTIVE

To evaluate and comparison of 2 commercial ELISA kits (Vrion/Serion kit and IBL kit) which ELISA kit will be used in sero-epidemiological survey in China in 2006 by Plaque Reduction Neutralization Test (PRNT).

METHODS

52 serum panel which contain different levels of measles antibody were used.

RESULTS

The results showed that the sensitivity, PPV and accurate of Virion/Serion kit achieved highest score when taking PRNT as "gold standard". Virion/Serion kit showed good relationship with PRNT titer: the correlation coefficients are 0.878 (P<0.01). IBL kit get lower correlation coefficients 0.850 (P<0.01). The test value and mean of unit of serum antibody increased along with neutralizing titers increased.

CONCLUSION

The Virion/Serion kit is good in sensitivity and specificity. It has quantitative for single well. The kit is suitable for sera epidemiology survey to detection of automatic workstation in China.

Authors+Show Affiliations

World Health Organization Western Pacific Region Regional Reference Measles Laboratory State Key Laboratory for Molecular Virology & Genetic Engineering, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100050, China.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

20084884

Citation

Mao, Nai-ying, et al. "[Comparison and Evaluation of Enzyme-linked Immunization Assay Kits With Plague Reduction Neutralization Test for Detection of Measles IgG Antibody]." Zhongguo Yi Miao He Mian Yi, vol. 15, no. 3, 2009, pp. 215-8.
Mao NY, Zhu Z, Jiang XH. [Comparison and evaluation of enzyme-linked immunization assay kits with plague reduction neutralization test for detection of measles IgG antibody]. Zhongguo Yi Miao He Mian Yi. 2009;15(3):215-8.
Mao, N. Y., Zhu, Z., & Jiang, X. H. (2009). [Comparison and evaluation of enzyme-linked immunization assay kits with plague reduction neutralization test for detection of measles IgG antibody]. Zhongguo Yi Miao He Mian Yi, 15(3), 215-8.
Mao NY, Zhu Z, Jiang XH. [Comparison and Evaluation of Enzyme-linked Immunization Assay Kits With Plague Reduction Neutralization Test for Detection of Measles IgG Antibody]. Zhongguo Yi Miao He Mian Yi. 2009;15(3):215-8. PubMed PMID: 20084884.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Comparison and evaluation of enzyme-linked immunization assay kits with plague reduction neutralization test for detection of measles IgG antibody]. AU - Mao,Nai-ying, AU - Zhu,Zhen, AU - Jiang,Xiao-hong, PY - 2010/1/21/entrez PY - 2010/1/21/pubmed PY - 2010/2/17/medline SP - 215 EP - 8 JF - Zhongguo yi miao he mian yi JO - Zhongguo Yi Miao He Mian Yi VL - 15 IS - 3 N2 - OBJECTIVE: To evaluate and comparison of 2 commercial ELISA kits (Vrion/Serion kit and IBL kit) which ELISA kit will be used in sero-epidemiological survey in China in 2006 by Plaque Reduction Neutralization Test (PRNT). METHODS: 52 serum panel which contain different levels of measles antibody were used. RESULTS: The results showed that the sensitivity, PPV and accurate of Virion/Serion kit achieved highest score when taking PRNT as "gold standard". Virion/Serion kit showed good relationship with PRNT titer: the correlation coefficients are 0.878 (P<0.01). IBL kit get lower correlation coefficients 0.850 (P<0.01). The test value and mean of unit of serum antibody increased along with neutralizing titers increased. CONCLUSION: The Virion/Serion kit is good in sensitivity and specificity. It has quantitative for single well. The kit is suitable for sera epidemiology survey to detection of automatic workstation in China. SN - 1006-916X UR - https://www.unboundmedicine.com/medline/citation/20084884/[Comparison_and_evaluation_of_enzyme_linked_immunization_assay_kits_with_plague_reduction_neutralization_test_for_detection_of_measles_IgG_antibody]_ L2 - http://www.diseaseinfosearch.org/result/4535 DB - PRIME DP - Unbound Medicine ER -