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Higher expression of vascular endothelial growth factor (VEGF) and its receptor VEGFR-2 (Flk-1) and metalloproteinase-9 (MMP-9) in a rat model of peritoneal endometriosis is similar to cancer diseases.
J Exp Clin Cancer Res. 2010 Jan 19; 29:4.JE

Abstract

BACKGROUND

Endometriosis is a common disease characterized by the presence of a functional endometrium outside the uterine cavity, causing pelvic pain, dysmenorrheal, and infertility. This disease has been associated to development of different types of malignancies; therefore new blood vessels are essential for the survival of the endometrial implant. Our previous observations on humans showed that angiogenesis is predominantly found in rectosigmoid endometriosis, a deeply infiltrating disease. In this study, we have established the experimental model of rat peritoneal endometriosis to evaluate the process of angiogenesis and to compare with eutopic endometrium.

METHODS

We have investigated the morphological characteristics of these lesions and the vascular density, VEGF and its receptor Flk-1 and MMP-9 expression, and activated macrophage distribution, using immunohistochemistry and RT-PCR.

RESULTS

As expected, the auto-transplantation of endometrium pieces into the peritoneal cavity is a well-established method for endometriosis induction in rats. The lesions were cystic and vascularized, and demonstrated histological hallmarks of human pathology, such as endometrial glands and stroma. The vascular density and the presence of VEGF and Flk-1 and MMP-9 were significantly higher in endometriotic lesions than in eutopic endometrium, and confirmed the angiogenic potential of these lesions. We also observed an increase in the number of activated macrophages (ED-1 positive cells) in the endometriotic lesions, showing a positive correlation with VEGF.

CONCLUSION

The present endometriosis model would be useful for investigation of the mechanisms of angiogenesis process involved in the peritoneal attachment of endometrial cells, as well as of the effects of therapeutic drugs, particularly with antiangiogenic activity.

Authors+Show Affiliations

Programa de Pesquisa em Biologia Celular e do Desenvolvimento, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Cidade Universitária-Ilha do Fundão, 21941-590 Rio de Janeiro, RJ Brazil.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20085636

Citation

Machado, Daniel E., et al. "Higher Expression of Vascular Endothelial Growth Factor (VEGF) and Its Receptor VEGFR-2 (Flk-1) and Metalloproteinase-9 (MMP-9) in a Rat Model of Peritoneal Endometriosis Is Similar to Cancer Diseases." Journal of Experimental & Clinical Cancer Research : CR, vol. 29, 2010, p. 4.
Machado DE, Berardo PT, Palmero CY, et al. Higher expression of vascular endothelial growth factor (VEGF) and its receptor VEGFR-2 (Flk-1) and metalloproteinase-9 (MMP-9) in a rat model of peritoneal endometriosis is similar to cancer diseases. J Exp Clin Cancer Res. 2010;29:4.
Machado, D. E., Berardo, P. T., Palmero, C. Y., & Nasciutti, L. E. (2010). Higher expression of vascular endothelial growth factor (VEGF) and its receptor VEGFR-2 (Flk-1) and metalloproteinase-9 (MMP-9) in a rat model of peritoneal endometriosis is similar to cancer diseases. Journal of Experimental & Clinical Cancer Research : CR, 29, 4. https://doi.org/10.1186/1756-9966-29-4
Machado DE, et al. Higher Expression of Vascular Endothelial Growth Factor (VEGF) and Its Receptor VEGFR-2 (Flk-1) and Metalloproteinase-9 (MMP-9) in a Rat Model of Peritoneal Endometriosis Is Similar to Cancer Diseases. J Exp Clin Cancer Res. 2010 Jan 19;29:4. PubMed PMID: 20085636.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Higher expression of vascular endothelial growth factor (VEGF) and its receptor VEGFR-2 (Flk-1) and metalloproteinase-9 (MMP-9) in a rat model of peritoneal endometriosis is similar to cancer diseases. AU - Machado,Daniel E, AU - Berardo,Plínio T, AU - Palmero,Celia Y, AU - Nasciutti,Luiz E, Y1 - 2010/01/19/ PY - 2009/12/02/received PY - 2010/01/19/accepted PY - 2010/1/21/entrez PY - 2010/1/21/pubmed PY - 2010/6/18/medline SP - 4 EP - 4 JF - Journal of experimental & clinical cancer research : CR JO - J. Exp. Clin. Cancer Res. VL - 29 N2 - BACKGROUND: Endometriosis is a common disease characterized by the presence of a functional endometrium outside the uterine cavity, causing pelvic pain, dysmenorrheal, and infertility. This disease has been associated to development of different types of malignancies; therefore new blood vessels are essential for the survival of the endometrial implant. Our previous observations on humans showed that angiogenesis is predominantly found in rectosigmoid endometriosis, a deeply infiltrating disease. In this study, we have established the experimental model of rat peritoneal endometriosis to evaluate the process of angiogenesis and to compare with eutopic endometrium. METHODS: We have investigated the morphological characteristics of these lesions and the vascular density, VEGF and its receptor Flk-1 and MMP-9 expression, and activated macrophage distribution, using immunohistochemistry and RT-PCR. RESULTS: As expected, the auto-transplantation of endometrium pieces into the peritoneal cavity is a well-established method for endometriosis induction in rats. The lesions were cystic and vascularized, and demonstrated histological hallmarks of human pathology, such as endometrial glands and stroma. The vascular density and the presence of VEGF and Flk-1 and MMP-9 were significantly higher in endometriotic lesions than in eutopic endometrium, and confirmed the angiogenic potential of these lesions. We also observed an increase in the number of activated macrophages (ED-1 positive cells) in the endometriotic lesions, showing a positive correlation with VEGF. CONCLUSION: The present endometriosis model would be useful for investigation of the mechanisms of angiogenesis process involved in the peritoneal attachment of endometrial cells, as well as of the effects of therapeutic drugs, particularly with antiangiogenic activity. SN - 1756-9966 UR - https://www.unboundmedicine.com/medline/citation/20085636/Higher_expression_of_vascular_endothelial_growth_factor__VEGF__and_its_receptor_VEGFR_2__Flk_1__and_metalloproteinase_9__MMP_9__in_a_rat_model_of_peritoneal_endometriosis_is_similar_to_cancer_diseases_ L2 - https://jeccr.biomedcentral.com/articles/10.1186/1756-9966-29-4 DB - PRIME DP - Unbound Medicine ER -