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A new genotype 2 subcluster identified among GBV-C strains circulating in the Lisbon metropolitan area of Portugal.
J Med Virol. 2010 Mar; 82(3):452-9.JM

Abstract

The rate of infection by the GBV-C virus was investigated in a group of 214 individuals at high risk of infection with parenterally transmitted viruses, and all living in the Lisbon metropolitan area (Portugal). RNA was extracted from plasma samples, and a fragment of the 5'-UTR was amplified by RT-PCR, disclosing a high prevalence of infection (40.7%). Most probably due to similar modes of viral transmission, the majority of GBV-C (+) individuals were found to be coinfected with HIV and/or HCV. A genomic region covering part of the E1/E2 glycoprotein coding sequence was amplified from approximately half of the GBV-C positive samples (44/87). Phylogenetic analysis of nucleotide sequences showed segregation of Portuguese GBV-C strains with genotype 1 (G1, n = 10) and genotype 2 (G2, n = 24) references. Genotype 1 was significantly associated with the African descent of those infected. Curiously, some of the strains assigned to genotype 2 were shown to form a separate cluster (designated G2*) in both neighbor-joining and Bayesian phylogenetic trees, which was confirmed by multivariate principal coordinate analysis. However, analysis of the distribution of intra- and intergenotype genetic distances support the hypothesis that rather than corresponding to a new viral genotype, G2* is a geographical subcluster within the genotype 2 radiation. J. Med. Virol. 82:452-459, 2010. (c) 2010 Wiley-Liss, Inc.

Authors+Show Affiliations

Virology Unit/Parasitology and Medical Microbiology Unit, Institute of Hygiene and Tropical Medicine, New Lisbon University (UNL), Lisboa, Portugal.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20087931

Citation

Branco, Cristina, et al. "A New Genotype 2 Subcluster Identified Among GBV-C Strains Circulating in the Lisbon Metropolitan Area of Portugal." Journal of Medical Virology, vol. 82, no. 3, 2010, pp. 452-9.
Branco C, Esteves A, Piedade J, et al. A new genotype 2 subcluster identified among GBV-C strains circulating in the Lisbon metropolitan area of Portugal. J Med Virol. 2010;82(3):452-9.
Branco, C., Esteves, A., Piedade, J., & Parreira, R. (2010). A new genotype 2 subcluster identified among GBV-C strains circulating in the Lisbon metropolitan area of Portugal. Journal of Medical Virology, 82(3), 452-9. https://doi.org/10.1002/jmv.21703
Branco C, et al. A New Genotype 2 Subcluster Identified Among GBV-C Strains Circulating in the Lisbon Metropolitan Area of Portugal. J Med Virol. 2010;82(3):452-9. PubMed PMID: 20087931.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A new genotype 2 subcluster identified among GBV-C strains circulating in the Lisbon metropolitan area of Portugal. AU - Branco,Cristina, AU - Esteves,Aida, AU - Piedade,João, AU - Parreira,Ricardo, PY - 2010/1/21/entrez PY - 2010/1/21/pubmed PY - 2010/3/18/medline SP - 452 EP - 9 JF - Journal of medical virology JO - J Med Virol VL - 82 IS - 3 N2 - The rate of infection by the GBV-C virus was investigated in a group of 214 individuals at high risk of infection with parenterally transmitted viruses, and all living in the Lisbon metropolitan area (Portugal). RNA was extracted from plasma samples, and a fragment of the 5'-UTR was amplified by RT-PCR, disclosing a high prevalence of infection (40.7%). Most probably due to similar modes of viral transmission, the majority of GBV-C (+) individuals were found to be coinfected with HIV and/or HCV. A genomic region covering part of the E1/E2 glycoprotein coding sequence was amplified from approximately half of the GBV-C positive samples (44/87). Phylogenetic analysis of nucleotide sequences showed segregation of Portuguese GBV-C strains with genotype 1 (G1, n = 10) and genotype 2 (G2, n = 24) references. Genotype 1 was significantly associated with the African descent of those infected. Curiously, some of the strains assigned to genotype 2 were shown to form a separate cluster (designated G2*) in both neighbor-joining and Bayesian phylogenetic trees, which was confirmed by multivariate principal coordinate analysis. However, analysis of the distribution of intra- and intergenotype genetic distances support the hypothesis that rather than corresponding to a new viral genotype, G2* is a geographical subcluster within the genotype 2 radiation. J. Med. Virol. 82:452-459, 2010. (c) 2010 Wiley-Liss, Inc. SN - 1096-9071 UR - https://www.unboundmedicine.com/medline/citation/20087931/A_new_genotype_2_subcluster_identified_among_GBV_C_strains_circulating_in_the_Lisbon_metropolitan_area_of_Portugal_ L2 - https://doi.org/10.1002/jmv.21703 DB - PRIME DP - Unbound Medicine ER -