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Spread of plasmids containing the bla(VIM-1) and bla(CTX-M) genes and the qnr determinant in Enterobacter cloacae, Klebsiella pneumoniae and Klebsiella oxytoca isolates.
J Antimicrob Chemother. 2010 Apr; 65(4):661-5.JA

Abstract

OBJECTIVES

We describe 12 VIM-1-producing strains (7 Enterobacter cloacae, 2 Klebsiella pneumoniae and 3 clonal Klebsiella oxytoca strains) detected among clinically relevant Enterobacteriaceae isolates from routine cultures at the Hospital del Mar (Barcelona, Spain) from December 2006 to May 2007.

METHODS

Susceptibility to carbapenems was evaluated with the MicroScan system. beta-Lactamases were identified by PCR and sequencing. Clonal relationships between the isolates were analysed by PFGE. Transferability of the enzymes was tested by conjugation. Plasmid characterization was performed by PCR-based replicon typing and PFGE with S1 nuclease digestion of whole genomic DNA. The PFGE gels were then transferred and hybridized.

RESULTS

The disc diffusion method correctly identified five of the seven E. cloacae isolates as intermediate or resistant strains. All isolates produced the VIM-1 enzyme. Three E. cloacae and three K. oxytoca strains were also CTX-M-9-producing strains, and one E. cloacae was also a CTX-M-3-producing strain. The plasmids carrying the bla(VIM) gene, of unknown incompatibility group, had a size of approximately 75 kb (eight strains) or 40 kb (three strains) and also contained the qnrS and the aac(6')-Ib-cr genes. In the remaining strain the bla(VIM-1) gene was found in an HI2 plasmid of 290 kb together with bla(CTX-M-9), qnrA, qnrS and the aac(6')-Ib-cr genes.

CONCLUSIONS

The results showed a linkage between the bla(VIM-1) and the qnrS and the aac(6')-Ib-cr genes, and between the bla(CTX-M-9) and the qnrA genes.

Authors+Show Affiliations

Servei de Microbiologia, Hospital de Sant Pau, Barcelona, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20089541

Citation

Miró, Elisenda, et al. "Spread of Plasmids Containing the bla(VIM-1) and bla(CTX-M) Genes and the Qnr Determinant in Enterobacter Cloacae, Klebsiella Pneumoniae and Klebsiella Oxytoca Isolates." The Journal of Antimicrobial Chemotherapy, vol. 65, no. 4, 2010, pp. 661-5.
Miró E, Segura C, Navarro F, et al. Spread of plasmids containing the bla(VIM-1) and bla(CTX-M) genes and the qnr determinant in Enterobacter cloacae, Klebsiella pneumoniae and Klebsiella oxytoca isolates. J Antimicrob Chemother. 2010;65(4):661-5.
Miró, E., Segura, C., Navarro, F., Sorlí, L., Coll, P., Horcajada, J. P., Alvarez-Lerma, F., & Salvadó, M. (2010). Spread of plasmids containing the bla(VIM-1) and bla(CTX-M) genes and the qnr determinant in Enterobacter cloacae, Klebsiella pneumoniae and Klebsiella oxytoca isolates. The Journal of Antimicrobial Chemotherapy, 65(4), 661-5. https://doi.org/10.1093/jac/dkp504
Miró E, et al. Spread of Plasmids Containing the bla(VIM-1) and bla(CTX-M) Genes and the Qnr Determinant in Enterobacter Cloacae, Klebsiella Pneumoniae and Klebsiella Oxytoca Isolates. J Antimicrob Chemother. 2010;65(4):661-5. PubMed PMID: 20089541.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Spread of plasmids containing the bla(VIM-1) and bla(CTX-M) genes and the qnr determinant in Enterobacter cloacae, Klebsiella pneumoniae and Klebsiella oxytoca isolates. AU - Miró,Elisenda, AU - Segura,Concha, AU - Navarro,Ferran, AU - Sorlí,Lluisa, AU - Coll,Pere, AU - Horcajada,Juan P, AU - Alvarez-Lerma,Francisco, AU - Salvadó,Margarita, Y1 - 2010/01/20/ PY - 2010/1/22/entrez PY - 2010/1/22/pubmed PY - 2010/5/26/medline SP - 661 EP - 5 JF - The Journal of antimicrobial chemotherapy JO - J. Antimicrob. Chemother. VL - 65 IS - 4 N2 - OBJECTIVES: We describe 12 VIM-1-producing strains (7 Enterobacter cloacae, 2 Klebsiella pneumoniae and 3 clonal Klebsiella oxytoca strains) detected among clinically relevant Enterobacteriaceae isolates from routine cultures at the Hospital del Mar (Barcelona, Spain) from December 2006 to May 2007. METHODS: Susceptibility to carbapenems was evaluated with the MicroScan system. beta-Lactamases were identified by PCR and sequencing. Clonal relationships between the isolates were analysed by PFGE. Transferability of the enzymes was tested by conjugation. Plasmid characterization was performed by PCR-based replicon typing and PFGE with S1 nuclease digestion of whole genomic DNA. The PFGE gels were then transferred and hybridized. RESULTS: The disc diffusion method correctly identified five of the seven E. cloacae isolates as intermediate or resistant strains. All isolates produced the VIM-1 enzyme. Three E. cloacae and three K. oxytoca strains were also CTX-M-9-producing strains, and one E. cloacae was also a CTX-M-3-producing strain. The plasmids carrying the bla(VIM) gene, of unknown incompatibility group, had a size of approximately 75 kb (eight strains) or 40 kb (three strains) and also contained the qnrS and the aac(6')-Ib-cr genes. In the remaining strain the bla(VIM-1) gene was found in an HI2 plasmid of 290 kb together with bla(CTX-M-9), qnrA, qnrS and the aac(6')-Ib-cr genes. CONCLUSIONS: The results showed a linkage between the bla(VIM-1) and the qnrS and the aac(6')-Ib-cr genes, and between the bla(CTX-M-9) and the qnrA genes. SN - 1460-2091 UR - https://www.unboundmedicine.com/medline/citation/20089541/Spread_of_plasmids_containing_the_bla_VIM_1__and_bla_CTX_M__genes_and_the_qnr_determinant_in_Enterobacter_cloacae_Klebsiella_pneumoniae_and_Klebsiella_oxytoca_isolates_ L2 - https://academic.oup.com/jac/article-lookup/doi/10.1093/jac/dkp504 DB - PRIME DP - Unbound Medicine ER -