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Molecular identity of periglomerular and short axon cells.
J Neurosci 2010; 30(3):1185-96JN

Abstract

Within glomeruli, the initial sites of synaptic integration in the olfactory pathway, olfactory sensory axons terminate on dendrites of projection and juxtaglomerular (JG) neurons. JG cells form at least two major circuits: the classic intraglomerular circuit consisting of external tufted (ET) and periglomerular (PG) cells and an interglomerular circuit comprised of the long-range connections of short axon (SA) cells. We examined the projections and the synaptic inputs of identified JG cell chemotypes using mice expressing green fluorescent protein (GFP) driven by the promoter for glutamic acid decarboxylase (GAD) 65 kDa, 67 kDa, or tyrosine hydroxylase (TH). Virtually all (97%) TH+ cells are also GAD67+ and are thus DAergic-GABAergic neurons. Using a combination of retrograde tracing, whole-cell patch-clamp recording, and single-cell three-dimensional reconstruction, we show that different JG cell chemotypes contribute to distinct microcircuits within or between glomeruli. GAD65+ GABAergic PG cells ramify principally within one glomerulus and participate in uniglomerular circuits. DAergic-GABAergic cells have extensive interglomerular projections. DAergic-GABAergic SA cells comprise two subgroups. One subpopulation contacts 5-12 glomeruli and is referred to as "oligoglomerular." Approximately one-third of these oligoglomerular DAergic SA cells receive direct olfactory nerve (ON) synaptic input, and the remaining two-thirds receive input via a disynaptic ON-->ET-->SA circuit. The second population of DAergic-GABAergic SA cells also disynaptic ON input and connect tens to hundreds of glomeruli in an extensive "polyglomerular" network. Although DAergic JG cells have traditionally been considered PG cells, their interglomerular connections argue that they are more appropriately classified as SA cells.

Authors+Show Affiliations

Department of Anatomy and Neurobiology, University of Maryland, School of Medicine, Baltimore, Maryland 21201, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

20089927

Citation

Kiyokage, Emi, et al. "Molecular Identity of Periglomerular and Short Axon Cells." The Journal of Neuroscience : the Official Journal of the Society for Neuroscience, vol. 30, no. 3, 2010, pp. 1185-96.
Kiyokage E, Pan YZ, Shao Z, et al. Molecular identity of periglomerular and short axon cells. J Neurosci. 2010;30(3):1185-96.
Kiyokage, E., Pan, Y. Z., Shao, Z., Kobayashi, K., Szabo, G., Yanagawa, Y., ... Shipley, M. T. (2010). Molecular identity of periglomerular and short axon cells. The Journal of Neuroscience : the Official Journal of the Society for Neuroscience, 30(3), pp. 1185-96. doi:10.1523/JNEUROSCI.3497-09.2010.
Kiyokage E, et al. Molecular Identity of Periglomerular and Short Axon Cells. J Neurosci. 2010 Jan 20;30(3):1185-96. PubMed PMID: 20089927.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular identity of periglomerular and short axon cells. AU - Kiyokage,Emi, AU - Pan,Yu-Zhen, AU - Shao,Zuoyi, AU - Kobayashi,Kazuto, AU - Szabo,Gabor, AU - Yanagawa,Yuchio, AU - Obata,Kunihiko, AU - Okano,Hideyuki, AU - Toida,Kazunori, AU - Puche,Adam C, AU - Shipley,Michael T, PY - 2010/1/22/entrez PY - 2010/1/22/pubmed PY - 2010/2/6/medline SP - 1185 EP - 96 JF - The Journal of neuroscience : the official journal of the Society for Neuroscience JO - J. Neurosci. VL - 30 IS - 3 N2 - Within glomeruli, the initial sites of synaptic integration in the olfactory pathway, olfactory sensory axons terminate on dendrites of projection and juxtaglomerular (JG) neurons. JG cells form at least two major circuits: the classic intraglomerular circuit consisting of external tufted (ET) and periglomerular (PG) cells and an interglomerular circuit comprised of the long-range connections of short axon (SA) cells. We examined the projections and the synaptic inputs of identified JG cell chemotypes using mice expressing green fluorescent protein (GFP) driven by the promoter for glutamic acid decarboxylase (GAD) 65 kDa, 67 kDa, or tyrosine hydroxylase (TH). Virtually all (97%) TH+ cells are also GAD67+ and are thus DAergic-GABAergic neurons. Using a combination of retrograde tracing, whole-cell patch-clamp recording, and single-cell three-dimensional reconstruction, we show that different JG cell chemotypes contribute to distinct microcircuits within or between glomeruli. GAD65+ GABAergic PG cells ramify principally within one glomerulus and participate in uniglomerular circuits. DAergic-GABAergic cells have extensive interglomerular projections. DAergic-GABAergic SA cells comprise two subgroups. One subpopulation contacts 5-12 glomeruli and is referred to as "oligoglomerular." Approximately one-third of these oligoglomerular DAergic SA cells receive direct olfactory nerve (ON) synaptic input, and the remaining two-thirds receive input via a disynaptic ON-->ET-->SA circuit. The second population of DAergic-GABAergic SA cells also disynaptic ON input and connect tens to hundreds of glomeruli in an extensive "polyglomerular" network. Although DAergic JG cells have traditionally been considered PG cells, their interglomerular connections argue that they are more appropriately classified as SA cells. SN - 1529-2401 UR - https://www.unboundmedicine.com/medline/citation/20089927/Molecular_identity_of_periglomerular_and_short_axon_cells_ L2 - http://www.jneurosci.org/cgi/pmidlookup?view=long&pmid=20089927 DB - PRIME DP - Unbound Medicine ER -