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CagA status and VacA subtypes of Helicobacter pylori in relation to histopathologic findings in Iranian population.
Indian J Pathol Microbiol. 2010 Jan-Mar; 53(1):24-7.IJ

Abstract

BACKGROUND/OBJECTIVE

The aim of this study was to detect dominant cagA/vacA genotypes of Helicobacter Pylori (H. pylori) and determine correlations between different cagA/vacA genotypes and histologic features of chronic gastritis in Iranian patients.

METHODS

Gastric biopsy was taken from 166 patients with nonulcer dyspepsia. The specimens were processed and DNA from each H. pylori isolate was extracted from multiple colony sweeps for identification of glmM gene. The vacA subtypes and cagA gene were tested by PCR . Histopathological features were recorded and graded according to partial Sydney system.

RESULTS

Of the 86 strains, 66 (76.7%) were cagA positive. The proportions of vacA gene subtypes s1, s2, m1 and m2 in the 78 strains isolated were 70.5%, 29.5%, 37.2% and 62.8%, respectively. About 83.3% of the vacA-positive strains had s1 allele. Twenty-six strains (33.3%) were positive for both cagA and m1 allele. Positive cagA status and vacA subtypes were not associated significantly with presence of neutrophil infiltration, intestinal metaplasia or H. pylori density. Only vacA s1 was significantly associated with more severe inflammation (P=0.02). The dominant genotype of H. pylori was vacA plus s1/m2. CagA gene positivity rate was not closely associated with severity of the disease.

CONCLUSION

H. pylori strains showing vacA s1 genotype were associated with more severe gastritis. These findings show that vacA genotyping may have clinical relevance in Iran.

Authors+Show Affiliations

Department of Pathology, Research Center for Gastroenterology and Liver Disease, Shaheed Beheshti University, MC, Tehran, Iran.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20090217

Citation

Molaei, Mahsa, et al. "CagA Status and VacA Subtypes of Helicobacter Pylori in Relation to Histopathologic Findings in Iranian Population." Indian Journal of Pathology & Microbiology, vol. 53, no. 1, 2010, pp. 24-7.
Molaei M, Foroughi F, Mashayekhi R, et al. CagA status and VacA subtypes of Helicobacter pylori in relation to histopathologic findings in Iranian population. Indian J Pathol Microbiol. 2010;53(1):24-7.
Molaei, M., Foroughi, F., Mashayekhi, R., Haghazali, M., Zojaji, H., Jafari, F., Dabiri, H., & Zali, M. R. (2010). CagA status and VacA subtypes of Helicobacter pylori in relation to histopathologic findings in Iranian population. Indian Journal of Pathology & Microbiology, 53(1), 24-7. https://doi.org/10.4103/0377-4929.59178
Molaei M, et al. CagA Status and VacA Subtypes of Helicobacter Pylori in Relation to Histopathologic Findings in Iranian Population. Indian J Pathol Microbiol. 2010 Jan-Mar;53(1):24-7. PubMed PMID: 20090217.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CagA status and VacA subtypes of Helicobacter pylori in relation to histopathologic findings in Iranian population. AU - Molaei,Mahsa, AU - Foroughi,Forough, AU - Mashayekhi,Reza, AU - Haghazali,Mehrdad, AU - Zojaji,Homayoon, AU - Jafari,Fereshteh, AU - Dabiri,Hossein, AU - Zali,Mohammad Reza, PY - 2010/1/22/entrez PY - 2010/1/22/pubmed PY - 2010/3/20/medline SP - 24 EP - 7 JF - Indian journal of pathology & microbiology JO - Indian J Pathol Microbiol VL - 53 IS - 1 N2 - BACKGROUND/OBJECTIVE: The aim of this study was to detect dominant cagA/vacA genotypes of Helicobacter Pylori (H. pylori) and determine correlations between different cagA/vacA genotypes and histologic features of chronic gastritis in Iranian patients. METHODS: Gastric biopsy was taken from 166 patients with nonulcer dyspepsia. The specimens were processed and DNA from each H. pylori isolate was extracted from multiple colony sweeps for identification of glmM gene. The vacA subtypes and cagA gene were tested by PCR . Histopathological features were recorded and graded according to partial Sydney system. RESULTS: Of the 86 strains, 66 (76.7%) were cagA positive. The proportions of vacA gene subtypes s1, s2, m1 and m2 in the 78 strains isolated were 70.5%, 29.5%, 37.2% and 62.8%, respectively. About 83.3% of the vacA-positive strains had s1 allele. Twenty-six strains (33.3%) were positive for both cagA and m1 allele. Positive cagA status and vacA subtypes were not associated significantly with presence of neutrophil infiltration, intestinal metaplasia or H. pylori density. Only vacA s1 was significantly associated with more severe inflammation (P=0.02). The dominant genotype of H. pylori was vacA plus s1/m2. CagA gene positivity rate was not closely associated with severity of the disease. CONCLUSION: H. pylori strains showing vacA s1 genotype were associated with more severe gastritis. These findings show that vacA genotyping may have clinical relevance in Iran. SN - 0974-5130 UR - https://www.unboundmedicine.com/medline/citation/20090217/CagA_status_and_VacA_subtypes_of_Helicobacter_pylori_in_relation_to_histopathologic_findings_in_Iranian_population_ L2 - http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2010;volume=53;issue=1;spage=24;epage=27;aulast=Molaei DB - PRIME DP - Unbound Medicine ER -