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Effect of dietary n-3 and n-6 fatty acids on fatty acid desaturation in rat liver.
Biochim Biophys Acta. 1991 Feb 26; 1082(1):57-62.BB

Abstract

To study the effect of high-fat diets with varying contents of n-3 and n-6 fatty acids on the metabolism of essential fatty acids, the rat liver microsomal fatty acid desaturases were measured. The rats were fed for 3 weeks with diets high in linseed oil (18:3(n-3)), sunflower seed oil (18:2(n-6)) or fish oil (20:5(n-3) and 22:6(n-3)) (20%, w/w) using pellet fed rats as a reference. The delta 6-desaturase using 18:2(n-6) or 18:3(n-3) as substrates was stimulated 1.5-2.5-fold by linseed or sunflower seed oil, compared to the pellet reference. The delta 5-desaturase was stimulated 3.5-fold with linseed oil and 2.5-fold with sunflower seed oil, while the delta 9-desaturase was inhibited by all the high-fat diets. The delta 6-, 5- and 9-desaturase activities were in all cases considerably reduced with fish oil as compared to linseed and sunflower seed oil diets. With pellet fed rats the rates were highest for delta 9-desaturation and in decreasing order lower for delta 5-desaturation, delta 6-desaturation with 18:3 (n-3) as substrate and finally delta 6-Desaturation with 18:2(n-6) as substrate. The content of 20:4(n-6) in liver phospholipids increased with the diets rich in 18:2(n-6), and was reduced for the fish oil diet enriched in 20:5 and 22:6(n-3) fatty acids. The amount of 20:5(n-3) in phospholipids was as high with linseed oil diet as with the fish oil diet, while the 22:6(n-3) content was only increased with the fish oil diet.

Authors+Show Affiliations

Institute for Nutrition Research, University of Oslo, Norway.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2009302

Citation

Christiansen, E N., et al. "Effect of Dietary N-3 and N-6 Fatty Acids On Fatty Acid Desaturation in Rat Liver." Biochimica Et Biophysica Acta, vol. 1082, no. 1, 1991, pp. 57-62.
Christiansen EN, Lund JS, Rørtveit T, et al. Effect of dietary n-3 and n-6 fatty acids on fatty acid desaturation in rat liver. Biochim Biophys Acta. 1991;1082(1):57-62.
Christiansen, E. N., Lund, J. S., Rørtveit, T., & Rustan, A. C. (1991). Effect of dietary n-3 and n-6 fatty acids on fatty acid desaturation in rat liver. Biochimica Et Biophysica Acta, 1082(1), 57-62.
Christiansen EN, et al. Effect of Dietary N-3 and N-6 Fatty Acids On Fatty Acid Desaturation in Rat Liver. Biochim Biophys Acta. 1991 Feb 26;1082(1):57-62. PubMed PMID: 2009302.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of dietary n-3 and n-6 fatty acids on fatty acid desaturation in rat liver. AU - Christiansen,E N, AU - Lund,J S, AU - Rørtveit,T, AU - Rustan,A C, PY - 1991/2/26/pubmed PY - 1991/2/26/medline PY - 1991/2/26/entrez SP - 57 EP - 62 JF - Biochimica et biophysica acta JO - Biochim Biophys Acta VL - 1082 IS - 1 N2 - To study the effect of high-fat diets with varying contents of n-3 and n-6 fatty acids on the metabolism of essential fatty acids, the rat liver microsomal fatty acid desaturases were measured. The rats were fed for 3 weeks with diets high in linseed oil (18:3(n-3)), sunflower seed oil (18:2(n-6)) or fish oil (20:5(n-3) and 22:6(n-3)) (20%, w/w) using pellet fed rats as a reference. The delta 6-desaturase using 18:2(n-6) or 18:3(n-3) as substrates was stimulated 1.5-2.5-fold by linseed or sunflower seed oil, compared to the pellet reference. The delta 5-desaturase was stimulated 3.5-fold with linseed oil and 2.5-fold with sunflower seed oil, while the delta 9-desaturase was inhibited by all the high-fat diets. The delta 6-, 5- and 9-desaturase activities were in all cases considerably reduced with fish oil as compared to linseed and sunflower seed oil diets. With pellet fed rats the rates were highest for delta 9-desaturation and in decreasing order lower for delta 5-desaturation, delta 6-desaturation with 18:3 (n-3) as substrate and finally delta 6-Desaturation with 18:2(n-6) as substrate. The content of 20:4(n-6) in liver phospholipids increased with the diets rich in 18:2(n-6), and was reduced for the fish oil diet enriched in 20:5 and 22:6(n-3) fatty acids. The amount of 20:5(n-3) in phospholipids was as high with linseed oil diet as with the fish oil diet, while the 22:6(n-3) content was only increased with the fish oil diet. SN - 0006-3002 UR - https://www.unboundmedicine.com/medline/citation/2009302/Effect_of_dietary_n_3_and_n_6_fatty_acids_on_fatty_acid_desaturation_in_rat_liver_ DB - PRIME DP - Unbound Medicine ER -