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Generation of mice with a conditional null allele for Tbx2.
Genesis. 2010 Mar; 48(3):195-9.G

Abstract

The T-box transcription factor Tbx2 plays important roles in patterning and development, and has been implicated in cell-cycle regulation and cancer. Conventional disruption of Tbx2 results in abnormalities of the heart, limbs, eye and other structures, and early fetal lethality. To gain insight into the role of Tbx2 in different tissues and at different stages of development, we have generated a conditional null allele of Tbx2 by flanking Exon 2 with loxP sites (Tbx2(fl2)). Homozygous Tbx2(fl2) mice are viable and fertile, indicating that the Tbx2(fl2) allele is a fully functional Tbx2 allele. Cre-mediated recombination, using a ubiquitously active CMV-Cre line, results in deletion of Exon 2 and loss of protein expression. Embryos homozygous for the recombined allele (Tbx2(Delta2)) show the same heart and limb defects as conventional Tbx2-deficient embryos. This Tbx2 conditional null allele will be a valuable tool to uncover tissue-specific roles of Tbx2 in development and disease.

Authors+Show Affiliations

Heart Failure Research Center, Academic Medical Center, Amsterdam, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20095052

Citation

Wakker, Vincent, et al. "Generation of Mice With a Conditional Null Allele for Tbx2." Genesis (New York, N.Y. : 2000), vol. 48, no. 3, 2010, pp. 195-9.
Wakker V, Brons JF, Aanhaanen WT, et al. Generation of mice with a conditional null allele for Tbx2. Genesis. 2010;48(3):195-9.
Wakker, V., Brons, J. F., Aanhaanen, W. T., van Roon, M. A., Moorman, A. F., & Christoffels, V. M. (2010). Generation of mice with a conditional null allele for Tbx2. Genesis (New York, N.Y. : 2000), 48(3), 195-9. https://doi.org/10.1002/dvg.20596
Wakker V, et al. Generation of Mice With a Conditional Null Allele for Tbx2. Genesis. 2010;48(3):195-9. PubMed PMID: 20095052.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Generation of mice with a conditional null allele for Tbx2. AU - Wakker,Vincent, AU - Brons,Janynke F, AU - Aanhaanen,Wim T J, AU - van Roon,Marian A, AU - Moorman,Antoon F M, AU - Christoffels,Vincent M, PY - 2010/1/23/entrez PY - 2010/1/23/pubmed PY - 2010/8/26/medline SP - 195 EP - 9 JF - Genesis (New York, N.Y. : 2000) JO - Genesis VL - 48 IS - 3 N2 - The T-box transcription factor Tbx2 plays important roles in patterning and development, and has been implicated in cell-cycle regulation and cancer. Conventional disruption of Tbx2 results in abnormalities of the heart, limbs, eye and other structures, and early fetal lethality. To gain insight into the role of Tbx2 in different tissues and at different stages of development, we have generated a conditional null allele of Tbx2 by flanking Exon 2 with loxP sites (Tbx2(fl2)). Homozygous Tbx2(fl2) mice are viable and fertile, indicating that the Tbx2(fl2) allele is a fully functional Tbx2 allele. Cre-mediated recombination, using a ubiquitously active CMV-Cre line, results in deletion of Exon 2 and loss of protein expression. Embryos homozygous for the recombined allele (Tbx2(Delta2)) show the same heart and limb defects as conventional Tbx2-deficient embryos. This Tbx2 conditional null allele will be a valuable tool to uncover tissue-specific roles of Tbx2 in development and disease. SN - 1526-968X UR - https://www.unboundmedicine.com/medline/citation/20095052/Generation_of_mice_with_a_conditional_null_allele_for_Tbx2_ L2 - https://doi.org/10.1002/dvg.20596 DB - PRIME DP - Unbound Medicine ER -