Tags

Type your tag names separated by a space and hit enter

Surface plasmon resonance based immunosensor for serological diagnosis of dengue virus infection.
J Pharm Biomed Anal. 2010 Jun 05; 52(2):255-9.JP

Abstract

Surface plasmon resonance (SPR) is a promising tool in sensor technology for biomedical applications. An SPR based immunosensor was established for label free and real time assay for the serological diagnosis of dengue virus infection employing the dengue virus antigen as the sensing element. The dengue virus antigen conjugated with bovine serum albumin is covalently immobilized on a gold sensor chip via activated self-assembled monolayer (SAM) of 11-mercaptoundecanoic acid, by amide coupling. Surface morphology of the biosensor was recorded using atomic force microscopy. Presence of dengue virus specific IgM antibodies in dengue positive sera was monitored by increase in resonance angle in direct immunoassay, whereas the principle of indirect competitive inhibition immunoassay was used to detect presence of dengue virus for early detection of the onset of dengue viral infection in clinical diagnostics. Results were compared with those obtained by MAC-ELISA. The regeneration was achieved by pepsin solution in glycin-HCl buffer (pH 2.2) and sensor surface displayed a high level of stability during repeated immunoreaction cycles. The proposed biosensor being simple, effective and based on utilization of natural antigen-antibody affinity, our study presents an encouraging scope for development of biosensors for diagnosis of dengue and dengue hemorrhagic fever (DHF) which continues to be a major health problem in the tropical and subtropical regions of world.

Authors+Show Affiliations

Biosensor Laboratory, Department of Chemistry, JNV University, Jodhpur 342033, Rajasthan, India. skumbhat@rediffmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20097030

Citation

Kumbhat, Sunita, et al. "Surface Plasmon Resonance Based Immunosensor for Serological Diagnosis of Dengue Virus Infection." Journal of Pharmaceutical and Biomedical Analysis, vol. 52, no. 2, 2010, pp. 255-9.
Kumbhat S, Sharma K, Gehlot R, et al. Surface plasmon resonance based immunosensor for serological diagnosis of dengue virus infection. J Pharm Biomed Anal. 2010;52(2):255-9.
Kumbhat, S., Sharma, K., Gehlot, R., Solanki, A., & Joshi, V. (2010). Surface plasmon resonance based immunosensor for serological diagnosis of dengue virus infection. Journal of Pharmaceutical and Biomedical Analysis, 52(2), 255-9. https://doi.org/10.1016/j.jpba.2010.01.001
Kumbhat S, et al. Surface Plasmon Resonance Based Immunosensor for Serological Diagnosis of Dengue Virus Infection. J Pharm Biomed Anal. 2010 Jun 5;52(2):255-9. PubMed PMID: 20097030.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Surface plasmon resonance based immunosensor for serological diagnosis of dengue virus infection. AU - Kumbhat,Sunita, AU - Sharma,Kavita, AU - Gehlot,Rakhee, AU - Solanki,Aruna, AU - Joshi,Vinod, Y1 - 2010/01/11/ PY - 2009/07/09/received PY - 2009/12/26/revised PY - 2010/01/05/accepted PY - 2010/1/26/entrez PY - 2010/1/26/pubmed PY - 2010/5/25/medline SP - 255 EP - 9 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 52 IS - 2 N2 - Surface plasmon resonance (SPR) is a promising tool in sensor technology for biomedical applications. An SPR based immunosensor was established for label free and real time assay for the serological diagnosis of dengue virus infection employing the dengue virus antigen as the sensing element. The dengue virus antigen conjugated with bovine serum albumin is covalently immobilized on a gold sensor chip via activated self-assembled monolayer (SAM) of 11-mercaptoundecanoic acid, by amide coupling. Surface morphology of the biosensor was recorded using atomic force microscopy. Presence of dengue virus specific IgM antibodies in dengue positive sera was monitored by increase in resonance angle in direct immunoassay, whereas the principle of indirect competitive inhibition immunoassay was used to detect presence of dengue virus for early detection of the onset of dengue viral infection in clinical diagnostics. Results were compared with those obtained by MAC-ELISA. The regeneration was achieved by pepsin solution in glycin-HCl buffer (pH 2.2) and sensor surface displayed a high level of stability during repeated immunoreaction cycles. The proposed biosensor being simple, effective and based on utilization of natural antigen-antibody affinity, our study presents an encouraging scope for development of biosensors for diagnosis of dengue and dengue hemorrhagic fever (DHF) which continues to be a major health problem in the tropical and subtropical regions of world. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/20097030/Surface_plasmon_resonance_based_immunosensor_for_serological_diagnosis_of_dengue_virus_infection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(10)00002-6 DB - PRIME DP - Unbound Medicine ER -