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A comparison of hypothermic machine perfusion versus static cold storage in an experimental model of renal ischemia reperfusion injury.
Transplantation. 2010 Apr 15; 89(7):830-7.T

Abstract

INTRODUCTION

There is increasing support for the use of hypothermic machine perfusion (HMP) in an attempt to reduce preservation injury. However, experimental evidence is needed to further examine the effects of HMP on renal ischemia reperfusion injury.

METHODS

Porcine kidneys were subjected to 10 min of warm ischemia followed by 18 hr of static cold storage with hyperosomolar citrate (HOC), histidine-tryptophan-ketoglutarate (HTK), or University of Wisconsin (UW) solutions or 18 hr HMP with Kidney Perfusion Solution using the Lifeport perfusion system. Renal function, oxidative damage, and morphology were assessed during 3 hr of reperfusion with autologous blood using an isolated organ perfusion system.

RESULTS

During reperfusion, intrarenal resistance was significantly lower in the HMP group compared with HOC and UW (area under the curve; HMP 3.8+/-1.7, HOC 9.1+/-4.3, UW 7.7+/-2.2, HTK 5.6+/-1.9 mm Hg/min; P=0.006), and creatinine clearance was significantly higher compared with the UW group (area under the curve creatinine clearance; HMP 9.8+/-7.3, HOC 2.2+/-1.7, UW 1.8+/-1.0, HTK 2.1+/-1.8 mL/min/100 g; P=0.004). Tubular function was significantly improved in the HMP group (P<0.05); however, levels of lipid peroxidation were significantly higher (P=0.005).

CONCLUSION

HMP demonstrated a reduced level of preservation injury compared with the static techniques resulting in improved renal and tubular function and less tubular cell inflammation during reperfusion.

Authors+Show Affiliations

Department of Infection, Immunity and Inflammation, Transplant Group, University of Leicester, Leicester General Hospital, Leicester, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20098357

Citation

Hosgood, Sarah A., et al. "A Comparison of Hypothermic Machine Perfusion Versus Static Cold Storage in an Experimental Model of Renal Ischemia Reperfusion Injury." Transplantation, vol. 89, no. 7, 2010, pp. 830-7.
Hosgood SA, Yang B, Bagul A, et al. A comparison of hypothermic machine perfusion versus static cold storage in an experimental model of renal ischemia reperfusion injury. Transplantation. 2010;89(7):830-7.
Hosgood, S. A., Yang, B., Bagul, A., Mohamed, I. H., & Nicholson, M. L. (2010). A comparison of hypothermic machine perfusion versus static cold storage in an experimental model of renal ischemia reperfusion injury. Transplantation, 89(7), 830-7. https://doi.org/10.1097/TP.0b013e3181cfa1d2
Hosgood SA, et al. A Comparison of Hypothermic Machine Perfusion Versus Static Cold Storage in an Experimental Model of Renal Ischemia Reperfusion Injury. Transplantation. 2010 Apr 15;89(7):830-7. PubMed PMID: 20098357.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A comparison of hypothermic machine perfusion versus static cold storage in an experimental model of renal ischemia reperfusion injury. AU - Hosgood,Sarah A, AU - Yang,Bin, AU - Bagul,Atul, AU - Mohamed,Ismail H, AU - Nicholson,Michael L, PY - 2010/1/26/entrez PY - 2010/1/26/pubmed PY - 2010/4/30/medline SP - 830 EP - 7 JF - Transplantation JO - Transplantation VL - 89 IS - 7 N2 - INTRODUCTION: There is increasing support for the use of hypothermic machine perfusion (HMP) in an attempt to reduce preservation injury. However, experimental evidence is needed to further examine the effects of HMP on renal ischemia reperfusion injury. METHODS: Porcine kidneys were subjected to 10 min of warm ischemia followed by 18 hr of static cold storage with hyperosomolar citrate (HOC), histidine-tryptophan-ketoglutarate (HTK), or University of Wisconsin (UW) solutions or 18 hr HMP with Kidney Perfusion Solution using the Lifeport perfusion system. Renal function, oxidative damage, and morphology were assessed during 3 hr of reperfusion with autologous blood using an isolated organ perfusion system. RESULTS: During reperfusion, intrarenal resistance was significantly lower in the HMP group compared with HOC and UW (area under the curve; HMP 3.8+/-1.7, HOC 9.1+/-4.3, UW 7.7+/-2.2, HTK 5.6+/-1.9 mm Hg/min; P=0.006), and creatinine clearance was significantly higher compared with the UW group (area under the curve creatinine clearance; HMP 9.8+/-7.3, HOC 2.2+/-1.7, UW 1.8+/-1.0, HTK 2.1+/-1.8 mL/min/100 g; P=0.004). Tubular function was significantly improved in the HMP group (P<0.05); however, levels of lipid peroxidation were significantly higher (P=0.005). CONCLUSION: HMP demonstrated a reduced level of preservation injury compared with the static techniques resulting in improved renal and tubular function and less tubular cell inflammation during reperfusion. SN - 1534-6080 UR - https://www.unboundmedicine.com/medline/citation/20098357/A_comparison_of_hypothermic_machine_perfusion_versus_static_cold_storage_in_an_experimental_model_of_renal_ischemia_reperfusion_injury_ L2 - http://dx.doi.org/10.1097/TP.0b013e3181cfa1d2 DB - PRIME DP - Unbound Medicine ER -