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Chemiluminescence DNA biosensor based on dual-amplification of thrombin and thiocyanuric acid-gold nanoparticle network.
Analyst. 2010 Feb; 135(2):332-6.A

Abstract

A sensitive DNA biosensor based on dual-amplification of thrombin and thiocyanuric acid-gold nanoparticle (TCA-AuNP) network is developed. First, the sandwich hybridization is formed by the capture probe immobilized on the surface of magnetic beads (MBs), the target DNA and the reporter probe loaded on PbS nanoparticles (PbS NPs). The PbS NPs contain two kinds of DNA sequences, one is the reporter probe complementary to the target DNA, and the other is the thrombin aptamer I. Through the specific recognition for thrombin, thrombin aptamer II labeled gold nanoparticles are linked to the sandwich complex, and further fabricate a network with TCA. AuNPs are released and dissolved into Au(3+), which catalyzes luminol chemiluminescence (CL) reaction. Due to the dual-amplification effects of thrombin-labeled PbS NPs and the TCA-AuNP network, a significant sensitivity enhancement of this DNA biosensor could be obtained, in the range of 2.0 x 10(-16) M to 3.5 x 10(-14) M, with a limit of detection (LOD) as low as 1.0 x 10(-16) M.

Authors+Show Affiliations

Key Laboratory of Eco-chemical Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20098767

Citation

Li, Xuemei, et al. "Chemiluminescence DNA Biosensor Based On Dual-amplification of Thrombin and Thiocyanuric Acid-gold Nanoparticle Network." The Analyst, vol. 135, no. 2, 2010, pp. 332-6.
Li X, Li W, Zhang S. Chemiluminescence DNA biosensor based on dual-amplification of thrombin and thiocyanuric acid-gold nanoparticle network. Analyst. 2010;135(2):332-6.
Li, X., Li, W., & Zhang, S. (2010). Chemiluminescence DNA biosensor based on dual-amplification of thrombin and thiocyanuric acid-gold nanoparticle network. The Analyst, 135(2), 332-6. https://doi.org/10.1039/b921167e
Li X, Li W, Zhang S. Chemiluminescence DNA Biosensor Based On Dual-amplification of Thrombin and Thiocyanuric Acid-gold Nanoparticle Network. Analyst. 2010;135(2):332-6. PubMed PMID: 20098767.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chemiluminescence DNA biosensor based on dual-amplification of thrombin and thiocyanuric acid-gold nanoparticle network. AU - Li,Xuemei, AU - Li,Wei, AU - Zhang,Shusheng, Y1 - 2009/12/11/ PY - 2010/1/26/entrez PY - 2010/1/26/pubmed PY - 2010/2/26/medline SP - 332 EP - 6 JF - The Analyst JO - Analyst VL - 135 IS - 2 N2 - A sensitive DNA biosensor based on dual-amplification of thrombin and thiocyanuric acid-gold nanoparticle (TCA-AuNP) network is developed. First, the sandwich hybridization is formed by the capture probe immobilized on the surface of magnetic beads (MBs), the target DNA and the reporter probe loaded on PbS nanoparticles (PbS NPs). The PbS NPs contain two kinds of DNA sequences, one is the reporter probe complementary to the target DNA, and the other is the thrombin aptamer I. Through the specific recognition for thrombin, thrombin aptamer II labeled gold nanoparticles are linked to the sandwich complex, and further fabricate a network with TCA. AuNPs are released and dissolved into Au(3+), which catalyzes luminol chemiluminescence (CL) reaction. Due to the dual-amplification effects of thrombin-labeled PbS NPs and the TCA-AuNP network, a significant sensitivity enhancement of this DNA biosensor could be obtained, in the range of 2.0 x 10(-16) M to 3.5 x 10(-14) M, with a limit of detection (LOD) as low as 1.0 x 10(-16) M. SN - 1364-5528 UR - https://www.unboundmedicine.com/medline/citation/20098767/Chemiluminescence_DNA_biosensor_based_on_dual_amplification_of_thrombin_and_thiocyanuric_acid_gold_nanoparticle_network_ L2 - https://doi.org/10.1039/b921167e DB - PRIME DP - Unbound Medicine ER -