Tags

Type your tag names separated by a space and hit enter

Estimation of DNA integrity in blood cells of eastern mosquitofish (Gambusia holbrooki) inhabiting an aluminium-polluted water environment: an alkaline comet assay study.
Arch Environ Contam Toxicol. 2010 Aug; 59(2):182-93.AE

Abstract

To estimate the impacts of an Al-contaminated aquatic environment on DNA integrity in the blood cells of eastern mosquitofish Gambusia holbrooki Girard 1859 inhabiting Lake Njivice (Island of Krk, Croatia), an evaluation using the alkaline comet assay was carried out. Genome integrity was studied in parallel with the same fish species inhabiting the nearby, unpolluted Lake Ponikve. The amount of DNA damage in cells was estimated from three different parameters: comet tail length as the extent of genetic material migration, tail intensity (% DNA in the comet tail) and tail moment. The results indicate the loss of genome integrity in blood cells of mosquitofish inhabiting Lake Njivice and the genotoxicity of this aquatic environment. Using the same assay, acute genotoxicity of contaminated water and sediment was evaluated and confirmed on fish, mouse and human blood cells treated ex vivo. Results of the present study indicate that the alkaline comet assay applied to fish blood cells is a valuable tool for determining the potential genotoxicity of water pollutants and confirm its usefulness in the evaluation of DNA damage in fish living in Al-polluted waters.

Authors+Show Affiliations

Department of Zoology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, 10 000, Zagreb, Croatia. ivancica@zg.biol.pmf.hrNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20098983

Citation

Ternjej, Ivancica, et al. "Estimation of DNA Integrity in Blood Cells of Eastern Mosquitofish (Gambusia Holbrooki) Inhabiting an Aluminium-polluted Water Environment: an Alkaline Comet Assay Study." Archives of Environmental Contamination and Toxicology, vol. 59, no. 2, 2010, pp. 182-93.
Ternjej I, Mihaljević Z, Stanković I, et al. Estimation of DNA integrity in blood cells of eastern mosquitofish (Gambusia holbrooki) inhabiting an aluminium-polluted water environment: an alkaline comet assay study. Arch Environ Contam Toxicol. 2010;59(2):182-93.
Ternjej, I., Mihaljević, Z., Stanković, I., Kerovec, M., Sipos, L., Zeljezić, D., & Kopjar, N. (2010). Estimation of DNA integrity in blood cells of eastern mosquitofish (Gambusia holbrooki) inhabiting an aluminium-polluted water environment: an alkaline comet assay study. Archives of Environmental Contamination and Toxicology, 59(2), 182-93. https://doi.org/10.1007/s00244-010-9469-6
Ternjej I, et al. Estimation of DNA Integrity in Blood Cells of Eastern Mosquitofish (Gambusia Holbrooki) Inhabiting an Aluminium-polluted Water Environment: an Alkaline Comet Assay Study. Arch Environ Contam Toxicol. 2010;59(2):182-93. PubMed PMID: 20098983.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Estimation of DNA integrity in blood cells of eastern mosquitofish (Gambusia holbrooki) inhabiting an aluminium-polluted water environment: an alkaline comet assay study. AU - Ternjej,Ivancica, AU - Mihaljević,Zlatko, AU - Stanković,Igor, AU - Kerovec,Mladen, AU - Sipos,Laszlo, AU - Zeljezić,Davor, AU - Kopjar,Nevenka, Y1 - 2010/01/23/ PY - 2009/01/08/received PY - 2010/01/04/accepted PY - 2010/1/26/entrez PY - 2010/1/26/pubmed PY - 2010/8/13/medline SP - 182 EP - 93 JF - Archives of environmental contamination and toxicology JO - Arch Environ Contam Toxicol VL - 59 IS - 2 N2 - To estimate the impacts of an Al-contaminated aquatic environment on DNA integrity in the blood cells of eastern mosquitofish Gambusia holbrooki Girard 1859 inhabiting Lake Njivice (Island of Krk, Croatia), an evaluation using the alkaline comet assay was carried out. Genome integrity was studied in parallel with the same fish species inhabiting the nearby, unpolluted Lake Ponikve. The amount of DNA damage in cells was estimated from three different parameters: comet tail length as the extent of genetic material migration, tail intensity (% DNA in the comet tail) and tail moment. The results indicate the loss of genome integrity in blood cells of mosquitofish inhabiting Lake Njivice and the genotoxicity of this aquatic environment. Using the same assay, acute genotoxicity of contaminated water and sediment was evaluated and confirmed on fish, mouse and human blood cells treated ex vivo. Results of the present study indicate that the alkaline comet assay applied to fish blood cells is a valuable tool for determining the potential genotoxicity of water pollutants and confirm its usefulness in the evaluation of DNA damage in fish living in Al-polluted waters. SN - 1432-0703 UR - https://www.unboundmedicine.com/medline/citation/20098983/Estimation_of_DNA_integrity_in_blood_cells_of_eastern_mosquitofish__Gambusia_holbrooki__inhabiting_an_aluminium_polluted_water_environment:_an_alkaline_comet_assay_study_ L2 - https://dx.doi.org/10.1007/s00244-010-9469-6 DB - PRIME DP - Unbound Medicine ER -