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Possible adverse effect of high delta-alpha-tocopherol intake on hepatic iron overload: enhanced production of vitamin C and the genotoxin, 8-hydroxy-2'- deoxyguanosine.
Toxicol Mech Methods. 2010 Feb; 20(2):96-104.TM

Abstract

Excess hepatic iron generates reactive oxygen species that result in oxidative stress and oxidative damage to the liver. Vitamins have hitherto been considered to be a possible remedy. The aim of this study was to determine if high doses of delta-alpha-tocopherol supplementation in iron overload would ameliorate the oxidative stress. Four groups of 20 male Wistar albino rats were studied: group 1 (control) was fed normal diet, group 2 (Fe) 0.75% Ferrocene iron, group 3 (FV gp) 0.75% Ferrocene/delta-alpha-tocopherol (10x RDA), group 4 (V gp) normal diet/delta-alpha-tocopherol. After 12 months, serum iron, reduced glutathione, catalase, vitamin C, Oxygen Radical Absorbance Capacity, lipid peroxidation, 8-hydroxy-2'-deoxyguanosine (8-OHdG), aspartate transaminase (AST), and alanine transaminase (ALT) were measured. Vitamin C levels were: F gp = 5.04 +/- 0.09; FV gp = 5.85 +/- 0.13 (micromol/l) (p < 0.05). 8-hydroxy-2'-deoxyguanosine levels were: F gp = 143.6 +/- 6.4; FV gp = 179.2 +/- 18.2 (ng/ml) (p < 0.05). Oxidative liver damage, as determined by serum AST and ALT levels, was not attenuated by alpha-tocopherol. A positive correlation existed between vitamin C and 8-OHdG, suggesting possible delta-alpha-tocopherol toxicity.

Authors+Show Affiliations

MRC/Molecular Hepatology Research Unit, Department of Medicine, University of the Witwatersrand, Johannesburg, South Africa. gasare@chs.edu.ghNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20100038

Citation

Asare, George A., et al. "Possible Adverse Effect of High Delta-alpha-tocopherol Intake On Hepatic Iron Overload: Enhanced Production of Vitamin C and the Genotoxin, 8-hydroxy-2'- Deoxyguanosine." Toxicology Mechanisms and Methods, vol. 20, no. 2, 2010, pp. 96-104.
Asare GA, Ntombini B, Kew MC, et al. Possible adverse effect of high delta-alpha-tocopherol intake on hepatic iron overload: enhanced production of vitamin C and the genotoxin, 8-hydroxy-2'- deoxyguanosine. Toxicol Mech Methods. 2010;20(2):96-104.
Asare, G. A., Ntombini, B., Kew, M. C., Kahler-Venter, C. P., & Nortey, E. N. (2010). Possible adverse effect of high delta-alpha-tocopherol intake on hepatic iron overload: enhanced production of vitamin C and the genotoxin, 8-hydroxy-2'- deoxyguanosine. Toxicology Mechanisms and Methods, 20(2), 96-104. https://doi.org/10.3109/15376510903572888
Asare GA, et al. Possible Adverse Effect of High Delta-alpha-tocopherol Intake On Hepatic Iron Overload: Enhanced Production of Vitamin C and the Genotoxin, 8-hydroxy-2'- Deoxyguanosine. Toxicol Mech Methods. 2010;20(2):96-104. PubMed PMID: 20100038.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Possible adverse effect of high delta-alpha-tocopherol intake on hepatic iron overload: enhanced production of vitamin C and the genotoxin, 8-hydroxy-2'- deoxyguanosine. AU - Asare,George A, AU - Ntombini,Bicky, AU - Kew,Michael C, AU - Kahler-Venter,Christina P, AU - Nortey,Ezekiel N, PY - 2010/1/27/entrez PY - 2010/1/27/pubmed PY - 2010/5/7/medline SP - 96 EP - 104 JF - Toxicology mechanisms and methods JO - Toxicol Mech Methods VL - 20 IS - 2 N2 - Excess hepatic iron generates reactive oxygen species that result in oxidative stress and oxidative damage to the liver. Vitamins have hitherto been considered to be a possible remedy. The aim of this study was to determine if high doses of delta-alpha-tocopherol supplementation in iron overload would ameliorate the oxidative stress. Four groups of 20 male Wistar albino rats were studied: group 1 (control) was fed normal diet, group 2 (Fe) 0.75% Ferrocene iron, group 3 (FV gp) 0.75% Ferrocene/delta-alpha-tocopherol (10x RDA), group 4 (V gp) normal diet/delta-alpha-tocopherol. After 12 months, serum iron, reduced glutathione, catalase, vitamin C, Oxygen Radical Absorbance Capacity, lipid peroxidation, 8-hydroxy-2'-deoxyguanosine (8-OHdG), aspartate transaminase (AST), and alanine transaminase (ALT) were measured. Vitamin C levels were: F gp = 5.04 +/- 0.09; FV gp = 5.85 +/- 0.13 (micromol/l) (p < 0.05). 8-hydroxy-2'-deoxyguanosine levels were: F gp = 143.6 +/- 6.4; FV gp = 179.2 +/- 18.2 (ng/ml) (p < 0.05). Oxidative liver damage, as determined by serum AST and ALT levels, was not attenuated by alpha-tocopherol. A positive correlation existed between vitamin C and 8-OHdG, suggesting possible delta-alpha-tocopherol toxicity. SN - 1537-6524 UR - https://www.unboundmedicine.com/medline/citation/20100038/Possible_adverse_effect_of_high_delta_alpha_tocopherol_intake_on_hepatic_iron_overload:_enhanced_production_of_vitamin_C_and_the_genotoxin_8_hydroxy_2'__deoxyguanosine_ L2 - https://www.tandfonline.com/doi/full/10.3109/15376510903572888 DB - PRIME DP - Unbound Medicine ER -