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Distribution of epicatechin metabolites in lymphoid tissues and testes of young rats with a cocoa-enriched diet.
Br J Nutr 2010; 103(10):1393-7BJ

Abstract

An increasing number of scientific studies support that flavanol-rich foods and beverages such as cocoa can promote human health, and are beneficial agents for the prevention of some diseases. Our previous studies showed that long-term cocoa intake enhances the antioxidant status in lymphoid organs and also modulates lymphocyte functionality in healthy young rats. Cocoa polyphenolic antioxidants seem to be the best candidates for those effects. However, data regarding polyphenol metabolites in tissues after a long-term cocoa intake are scarce. In the present study we mainly focus on the uptake and accumulation of epicatechin metabolites in lymphoid organs, including the thymus, spleen and mesenteric lymphoid nodes, as well as in the liver and testes after a diet rich in cocoa. Ten young weaned Wistar rats were fed randomly with a 10 % (w/w) cocoa diet or a control diet for 3 weeks, corresponding to their infancy and youth. Tissues were treated with a solid-phase extraction and analysed by liquid chromatography-tandem MS. The major compounds recovered in these tissues were glucuronide derivatives of epicatechin and methylepicatechin. The highest concentration of these metabolites was found in the thymus, testicles and liver, followed by lymphatic nodes and spleen. The high amount of epicatechin metabolites found in the thymus supports our previous findings showing its high antioxidant capacity compared with other tissues such as the spleen. Moreover, this is the first time that epicatechin metabolites have been found in high concentrations in the testes, confirming other studies that have suggested the testes as an important site of oxidation.

Authors+Show Affiliations

Nutrition and Food Science Department, XaRTA, INSA, Faculty of Pharmacy, University of Barcelona, 08028 Barcelona, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20100378

Citation

Urpi-Sarda, Mireia, et al. "Distribution of Epicatechin Metabolites in Lymphoid Tissues and Testes of Young Rats With a Cocoa-enriched Diet." The British Journal of Nutrition, vol. 103, no. 10, 2010, pp. 1393-7.
Urpi-Sarda M, Ramiro-Puig E, Khan N, et al. Distribution of epicatechin metabolites in lymphoid tissues and testes of young rats with a cocoa-enriched diet. Br J Nutr. 2010;103(10):1393-7.
Urpi-Sarda, M., Ramiro-Puig, E., Khan, N., Ramos-Romero, S., Llorach, R., Castell, M., ... Andres-Lacueva, C. (2010). Distribution of epicatechin metabolites in lymphoid tissues and testes of young rats with a cocoa-enriched diet. The British Journal of Nutrition, 103(10), pp. 1393-7. doi:10.1017/S0007114509993473.
Urpi-Sarda M, et al. Distribution of Epicatechin Metabolites in Lymphoid Tissues and Testes of Young Rats With a Cocoa-enriched Diet. Br J Nutr. 2010;103(10):1393-7. PubMed PMID: 20100378.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Distribution of epicatechin metabolites in lymphoid tissues and testes of young rats with a cocoa-enriched diet. AU - Urpi-Sarda,Mireia, AU - Ramiro-Puig,Emma, AU - Khan,Nasiruddin, AU - Ramos-Romero,Sara, AU - Llorach,Rafael, AU - Castell,Margarida, AU - Gonzalez-Manzano,Susana, AU - Santos-Buelga,Celestino, AU - Andres-Lacueva,Cristina, Y1 - 2010/01/26/ PY - 2010/1/27/entrez PY - 2010/1/27/pubmed PY - 2010/6/3/medline SP - 1393 EP - 7 JF - The British journal of nutrition JO - Br. J. Nutr. VL - 103 IS - 10 N2 - An increasing number of scientific studies support that flavanol-rich foods and beverages such as cocoa can promote human health, and are beneficial agents for the prevention of some diseases. Our previous studies showed that long-term cocoa intake enhances the antioxidant status in lymphoid organs and also modulates lymphocyte functionality in healthy young rats. Cocoa polyphenolic antioxidants seem to be the best candidates for those effects. However, data regarding polyphenol metabolites in tissues after a long-term cocoa intake are scarce. In the present study we mainly focus on the uptake and accumulation of epicatechin metabolites in lymphoid organs, including the thymus, spleen and mesenteric lymphoid nodes, as well as in the liver and testes after a diet rich in cocoa. Ten young weaned Wistar rats were fed randomly with a 10 % (w/w) cocoa diet or a control diet for 3 weeks, corresponding to their infancy and youth. Tissues were treated with a solid-phase extraction and analysed by liquid chromatography-tandem MS. The major compounds recovered in these tissues were glucuronide derivatives of epicatechin and methylepicatechin. The highest concentration of these metabolites was found in the thymus, testicles and liver, followed by lymphatic nodes and spleen. The high amount of epicatechin metabolites found in the thymus supports our previous findings showing its high antioxidant capacity compared with other tissues such as the spleen. Moreover, this is the first time that epicatechin metabolites have been found in high concentrations in the testes, confirming other studies that have suggested the testes as an important site of oxidation. SN - 1475-2662 UR - https://www.unboundmedicine.com/medline/citation/20100378/Distribution_of_epicatechin_metabolites_in_lymphoid_tissues_and_testes_of_young_rats_with_a_cocoa_enriched_diet_ L2 - https://www.cambridge.org/core/product/identifier/S0007114509993473/type/journal_article DB - PRIME DP - Unbound Medicine ER -