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In vivo real-time imaging of human duodenal mucosal structures in celiac disease using endocytoscopy.
Endoscopy. 2010 Mar; 42(3):191-6.E

Abstract

BACKGROUND AND AIMS

Celiac disease is a gluten-induced enteropathy whose diagnosis is based on histological evidence of villous atrophy. The diagnosis may be difficult if the orientation of histological sections is other than optimal. During upper gastrointestinal endoscopy we studied in vivo duodenal mucosa in patients with celiac disease using endocytoscopy, a novel diagnostic technique allowing in vivo real-time visualization of mucosa under x 450 magnification.

METHODS

Sixteen patients with documented celiac disease and seven controls without celiac disease were studied. Endocytoscopic images obtained from several fields were compared in a blinded fashion to standard histology.

RESULTS

Endocytoscopy showed three different patterns of in vivo histology: (1) the presence of normal-appearing, long, thin villi, lined with clearly distinguishable surface epithelial cells, considered to be normal duodenal mucosa (n = 15, all controls and eight celiac disease patients); (2) the presence of thick, shortened villi, reflecting partial villous atrophy (n = 4); and (3) the total absence of villi and the presence of enlarged crypt orifices, reflecting total villous atrophy (n = 4). Good concordance between endocytoscopy and standard histology was found in all 16 patients with celiac disease.

CONCLUSIONS

Endocytoscopy allows in vivo, real-time, noninvasive visualization and characterization of villous architecture and may be a promising method for in vivo evaluation of duodenal mucosa in celiac disease.

Authors+Show Affiliations

Institut des Maladies de l'Appareil Digestif - INSERM U913, CIC 04 et Service d'Hépato-Gastroentérologie, Hôtel Dieu, CHU de Nantes, 44093 Nantes, France. tamara.matysiakbudnik@chu-nantes.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20101565

Citation

Matysiak-Budnik, T, et al. "In Vivo Real-time Imaging of Human Duodenal Mucosal Structures in Celiac Disease Using Endocytoscopy." Endoscopy, vol. 42, no. 3, 2010, pp. 191-6.
Matysiak-Budnik T, Coron E, Mosnier JF, et al. In vivo real-time imaging of human duodenal mucosal structures in celiac disease using endocytoscopy. Endoscopy. 2010;42(3):191-6.
Matysiak-Budnik, T., Coron, E., Mosnier, J. F., Le Rhun, M., Inoue, H., & Galmiche, J. P. (2010). In vivo real-time imaging of human duodenal mucosal structures in celiac disease using endocytoscopy. Endoscopy, 42(3), 191-6. https://doi.org/10.1055/s-0029-1243838
Matysiak-Budnik T, et al. In Vivo Real-time Imaging of Human Duodenal Mucosal Structures in Celiac Disease Using Endocytoscopy. Endoscopy. 2010;42(3):191-6. PubMed PMID: 20101565.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vivo real-time imaging of human duodenal mucosal structures in celiac disease using endocytoscopy. AU - Matysiak-Budnik,T, AU - Coron,E, AU - Mosnier,J-F, AU - Le Rhun,M, AU - Inoue,H, AU - Galmiche,J-P, Y1 - 2010/01/25/ PY - 2010/1/27/entrez PY - 2010/1/27/pubmed PY - 2010/6/11/medline SP - 191 EP - 6 JF - Endoscopy JO - Endoscopy VL - 42 IS - 3 N2 - BACKGROUND AND AIMS: Celiac disease is a gluten-induced enteropathy whose diagnosis is based on histological evidence of villous atrophy. The diagnosis may be difficult if the orientation of histological sections is other than optimal. During upper gastrointestinal endoscopy we studied in vivo duodenal mucosa in patients with celiac disease using endocytoscopy, a novel diagnostic technique allowing in vivo real-time visualization of mucosa under x 450 magnification. METHODS: Sixteen patients with documented celiac disease and seven controls without celiac disease were studied. Endocytoscopic images obtained from several fields were compared in a blinded fashion to standard histology. RESULTS: Endocytoscopy showed three different patterns of in vivo histology: (1) the presence of normal-appearing, long, thin villi, lined with clearly distinguishable surface epithelial cells, considered to be normal duodenal mucosa (n = 15, all controls and eight celiac disease patients); (2) the presence of thick, shortened villi, reflecting partial villous atrophy (n = 4); and (3) the total absence of villi and the presence of enlarged crypt orifices, reflecting total villous atrophy (n = 4). Good concordance between endocytoscopy and standard histology was found in all 16 patients with celiac disease. CONCLUSIONS: Endocytoscopy allows in vivo, real-time, noninvasive visualization and characterization of villous architecture and may be a promising method for in vivo evaluation of duodenal mucosa in celiac disease. SN - 1438-8812 UR - https://www.unboundmedicine.com/medline/citation/20101565/In_vivo_real_time_imaging_of_human_duodenal_mucosal_structures_in_celiac_disease_using_endocytoscopy_ L2 - http://www.thieme-connect.com/DOI/DOI?10.1055/s-0029-1243838 DB - PRIME DP - Unbound Medicine ER -