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A solid-state electrochemiluminescence sensing platform for detection of adenosine based on ferrocene-labeled structure-switching signaling aptamer.
Anal Chim Acta. 2010 Jan 25; 658(2):128-32.AC

Abstract

A solid-state electrochemiluminescence sensing platform based on ferrocene-labeled structure-switching signaling aptamer (Fc-aptamer) for highly sensitive detection of small molecules is developed successfully using adenosine as a model analyte. Such special sensing platform included two main parts, an electrochemiluminescence (ECL) substrate and an ECL intensity switch. The ECL substrate was made by modifying the complex of Au nanoparticle and Ruthenium (II) tris-(bipyridine) (Ru(bpy)(3)(2+)-AuNPs) onto Au electrode. An anti-adenosine aptamer labeled by ferrocene acted as the ECL intensity switch. A short complementary ssDNA for the aptamer was applied to hybridizing with the aptamer, yielding a double-stranded complex of the aptamer and the ssDNA on the electrode surface. The introduction of adenosine triggered structure switching of the aptamer. As a result, the ssDNA was forced to dissociate from the sensing platform. Such structural change of the aptamer resulted in an obvious ECL intensity decrease due to the increased quenching effect of Fc to the ECL substrate. The analytic results were sensitive and specific.

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Publisher Full Text

Authors+Show Affiliations

Wang X
School of Public Health, Southeast University, Nanjing 210009, China.
Dong P
No affiliation info available
He P
No affiliation info available
Fang Y
No affiliation info available

MeSH

AdenosineAptamers, NucleotideBiphenyl CompoundsDNA, Single-StrandedElectrochemical TechniquesElectrodesFerrous CompoundsGoldLuminescent MeasurementsMetal NanoparticlesMetallocenesRuthenium

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20103085

Citation

Wang, Xiaoying, et al. "A Solid-state Electrochemiluminescence Sensing Platform for Detection of Adenosine Based On Ferrocene-labeled Structure-switching Signaling Aptamer." Analytica Chimica Acta, vol. 658, no. 2, 2010, pp. 128-32.
Wang X, Dong P, He P, et al. A solid-state electrochemiluminescence sensing platform for detection of adenosine based on ferrocene-labeled structure-switching signaling aptamer. Anal Chim Acta. 2010;658(2):128-32.
Wang, X., Dong, P., He, P., & Fang, Y. (2010). A solid-state electrochemiluminescence sensing platform for detection of adenosine based on ferrocene-labeled structure-switching signaling aptamer. Analytica Chimica Acta, 658(2), 128-32. https://doi.org/10.1016/j.aca.2009.11.007
Wang X, et al. A Solid-state Electrochemiluminescence Sensing Platform for Detection of Adenosine Based On Ferrocene-labeled Structure-switching Signaling Aptamer. Anal Chim Acta. 2010 Jan 25;658(2):128-32. PubMed PMID: 20103085.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A solid-state electrochemiluminescence sensing platform for detection of adenosine based on ferrocene-labeled structure-switching signaling aptamer. AU - Wang,Xiaoying, AU - Dong,Ping, AU - He,Pingang, AU - Fang,Yuzhi, Y1 - 2009/11/10/ PY - 2009/10/01/received PY - 2009/10/27/revised PY - 2009/11/03/accepted PY - 2010/1/28/entrez PY - 2010/1/28/pubmed PY - 2010/4/9/medline SP - 128 EP - 32 JF - Analytica chimica acta JO - Anal Chim Acta VL - 658 IS - 2 N2 - A solid-state electrochemiluminescence sensing platform based on ferrocene-labeled structure-switching signaling aptamer (Fc-aptamer) for highly sensitive detection of small molecules is developed successfully using adenosine as a model analyte. Such special sensing platform included two main parts, an electrochemiluminescence (ECL) substrate and an ECL intensity switch. The ECL substrate was made by modifying the complex of Au nanoparticle and Ruthenium (II) tris-(bipyridine) (Ru(bpy)(3)(2+)-AuNPs) onto Au electrode. An anti-adenosine aptamer labeled by ferrocene acted as the ECL intensity switch. A short complementary ssDNA for the aptamer was applied to hybridizing with the aptamer, yielding a double-stranded complex of the aptamer and the ssDNA on the electrode surface. The introduction of adenosine triggered structure switching of the aptamer. As a result, the ssDNA was forced to dissociate from the sensing platform. Such structural change of the aptamer resulted in an obvious ECL intensity decrease due to the increased quenching effect of Fc to the ECL substrate. The analytic results were sensitive and specific. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/20103085/A_solid_state_electrochemiluminescence_sensing_platform_for_detection_of_adenosine_based_on_ferrocene_labeled_structure_switching_signaling_aptamer_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(09)01466-4 DB - PRIME DP - Unbound Medicine ER -