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Nanofibrous membrane based tyrosinase-biosensor for the detection of phenolic compounds.
Anal Chim Acta. 2010 Feb 05; 659(1-2):133-6.AC

Abstract

A tyrosinase-modified electrode is described to be used as amperometric biosensor for the detection of phenolic compounds in food. The enzyme has been immobilized by drop-coating on a glassy carbon electrode covered by a polyamidic nanofibrous membrane prepared by electrospinning. With respect to others, the selectivity of the designed tyrosinase-biosensor resulted modified by the presence of the nanostructured coating which seems to affect the permeability of phenols as a function of the pH of the solution and of their dissociation constants. The biosensor exhibits a response time of 16 s, a detection limit of 0.05 microM, and a linearity up to 100 microM (slope: -304 nA microM(-1); intercept: -191 nA, r(2)=0.996, n=19). Among others, it can be successfully used for monitoring in real time the release kinetics of phenols encapsulated in polymeric microcapsules.

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Authors+Show Affiliations

Arecchi A
Dipartimento di Scienze e Tecnologie Alimentari, Università degli Studi di Milano, Via Celoria 2, 20133, Milan, Italy.
Scampicchio M
No affiliation info available
Drusch S
No affiliation info available
Mannino S
No affiliation info available

MeSH

Biosensing TechniquesElectrochemical TechniquesElectrodesEnzymes, ImmobilizedFlavonoidsKineticsMonophenol MonooxygenaseNanocompositesPhenolsPolyphenolsReproducibility of Results

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20103115

Citation

Arecchi, Alessandra, et al. "Nanofibrous Membrane Based Tyrosinase-biosensor for the Detection of Phenolic Compounds." Analytica Chimica Acta, vol. 659, no. 1-2, 2010, pp. 133-6.
Arecchi A, Scampicchio M, Drusch S, et al. Nanofibrous membrane based tyrosinase-biosensor for the detection of phenolic compounds. Anal Chim Acta. 2010;659(1-2):133-6.
Arecchi, A., Scampicchio, M., Drusch, S., & Mannino, S. (2010). Nanofibrous membrane based tyrosinase-biosensor for the detection of phenolic compounds. Analytica Chimica Acta, 659(1-2), 133-6. https://doi.org/10.1016/j.aca.2009.11.039
Arecchi A, et al. Nanofibrous Membrane Based Tyrosinase-biosensor for the Detection of Phenolic Compounds. Anal Chim Acta. 2010 Feb 5;659(1-2):133-6. PubMed PMID: 20103115.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nanofibrous membrane based tyrosinase-biosensor for the detection of phenolic compounds. AU - Arecchi,Alessandra, AU - Scampicchio,Matteo, AU - Drusch,Stephan, AU - Mannino,Saverio, Y1 - 2009/11/26/ PY - 2009/09/10/received PY - 2009/11/16/revised PY - 2009/11/17/accepted PY - 2010/1/28/entrez PY - 2010/1/28/pubmed PY - 2010/3/31/medline SP - 133 EP - 6 JF - Analytica chimica acta JO - Anal Chim Acta VL - 659 IS - 1-2 N2 - A tyrosinase-modified electrode is described to be used as amperometric biosensor for the detection of phenolic compounds in food. The enzyme has been immobilized by drop-coating on a glassy carbon electrode covered by a polyamidic nanofibrous membrane prepared by electrospinning. With respect to others, the selectivity of the designed tyrosinase-biosensor resulted modified by the presence of the nanostructured coating which seems to affect the permeability of phenols as a function of the pH of the solution and of their dissociation constants. The biosensor exhibits a response time of 16 s, a detection limit of 0.05 microM, and a linearity up to 100 microM (slope: -304 nA microM(-1); intercept: -191 nA, r(2)=0.996, n=19). Among others, it can be successfully used for monitoring in real time the release kinetics of phenols encapsulated in polymeric microcapsules. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/20103115/Nanofibrous_membrane_based_tyrosinase_biosensor_for_the_detection_of_phenolic_compounds_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(09)01550-5 DB - PRIME DP - Unbound Medicine ER -