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Amperometric biosensor based on tyrosinase immobilized onto multiwalled carbon nanotubes-cobalt phthalocyanine-silk fibroin film and its application to determine bisphenol A.
Anal Chim Acta. 2010 Feb 05; 659(1-2):144-50.AC

Abstract

An amperometric bisphenol A (BPA) biosensor was fabricated by immobilizing tyrosinase on multiwalled carbon nanotubes (MWNTs)-cobalt phthalocyanine (CoPc)-silk fibroin (SF) composite modified glassy carbon electrode (GCE). In MWNTs-CoPc-SF composite film, SF provided a biocompatible microenvironment for the tyrosinase to retain its bioactivity, MWNTs possessed excellent inherent conductivity to enhance the electron transfer rate and CoPc showed good electrocatalytic activity to electrooxidation of BPA. The cyclic voltammogram of BPA at this biosensor exhibited a well defined anodic peak at 0.625 V. Compared with bare GCE, the oxidation signal of BPA significantly increased; therefore, this oxidation signal was used to determine BPA. The effect factors were optimized and the electrochemical parameters were calculated. The possible oxidation mechanism was also discussed. Under optimum conditions, the oxidation current was proportional to BPA concentration in the range from 5.0 x 10(-8) to 3.0 x 10(-6) M with correlation coefficient of 0.9979 and detection limit of 3.0 x 10(-8) M (S/N=3). The proposed method was successfully applied to determine BPA in plastic products and the recovery was in the range from 95.36% to 104.39%.

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Authors+Show Affiliations

Yin H
College of Chemistry and Material Science, Shandong Agricultural University, Taian 271018, Shandong, China.
Zhou Y
No affiliation info available
Xu J
No affiliation info available
Ai S
No affiliation info available
Cui L
No affiliation info available
Zhu L
No affiliation info available

MeSH

Benzhydryl CompoundsBiosensing TechniquesElectrochemical TechniquesElectrodesEnzymes, ImmobilizedFibroinsIndolesLimit of DetectionMonophenol MonooxygenaseNanotubes, CarbonOrganometallic CompoundsPhenolsReproducibility of Results

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20103117

Citation

Yin, Huanshun, et al. "Amperometric Biosensor Based On Tyrosinase Immobilized Onto Multiwalled Carbon Nanotubes-cobalt Phthalocyanine-silk Fibroin Film and Its Application to Determine Bisphenol A." Analytica Chimica Acta, vol. 659, no. 1-2, 2010, pp. 144-50.
Yin H, Zhou Y, Xu J, et al. Amperometric biosensor based on tyrosinase immobilized onto multiwalled carbon nanotubes-cobalt phthalocyanine-silk fibroin film and its application to determine bisphenol A. Anal Chim Acta. 2010;659(1-2):144-50.
Yin, H., Zhou, Y., Xu, J., Ai, S., Cui, L., & Zhu, L. (2010). Amperometric biosensor based on tyrosinase immobilized onto multiwalled carbon nanotubes-cobalt phthalocyanine-silk fibroin film and its application to determine bisphenol A. Analytica Chimica Acta, 659(1-2), 144-50. https://doi.org/10.1016/j.aca.2009.11.051
Yin H, et al. Amperometric Biosensor Based On Tyrosinase Immobilized Onto Multiwalled Carbon Nanotubes-cobalt Phthalocyanine-silk Fibroin Film and Its Application to Determine Bisphenol A. Anal Chim Acta. 2010 Feb 5;659(1-2):144-50. PubMed PMID: 20103117.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Amperometric biosensor based on tyrosinase immobilized onto multiwalled carbon nanotubes-cobalt phthalocyanine-silk fibroin film and its application to determine bisphenol A. AU - Yin,Huanshun, AU - Zhou,Yunlei, AU - Xu,Jing, AU - Ai,Shiyun, AU - Cui,Lin, AU - Zhu,Lusheng, Y1 - 2009/12/03/ PY - 2009/09/08/received PY - 2009/11/17/revised PY - 2009/11/21/accepted PY - 2010/1/28/entrez PY - 2010/1/28/pubmed PY - 2010/3/31/medline SP - 144 EP - 50 JF - Analytica chimica acta JO - Anal Chim Acta VL - 659 IS - 1-2 N2 - An amperometric bisphenol A (BPA) biosensor was fabricated by immobilizing tyrosinase on multiwalled carbon nanotubes (MWNTs)-cobalt phthalocyanine (CoPc)-silk fibroin (SF) composite modified glassy carbon electrode (GCE). In MWNTs-CoPc-SF composite film, SF provided a biocompatible microenvironment for the tyrosinase to retain its bioactivity, MWNTs possessed excellent inherent conductivity to enhance the electron transfer rate and CoPc showed good electrocatalytic activity to electrooxidation of BPA. The cyclic voltammogram of BPA at this biosensor exhibited a well defined anodic peak at 0.625 V. Compared with bare GCE, the oxidation signal of BPA significantly increased; therefore, this oxidation signal was used to determine BPA. The effect factors were optimized and the electrochemical parameters were calculated. The possible oxidation mechanism was also discussed. Under optimum conditions, the oxidation current was proportional to BPA concentration in the range from 5.0 x 10(-8) to 3.0 x 10(-6) M with correlation coefficient of 0.9979 and detection limit of 3.0 x 10(-8) M (S/N=3). The proposed method was successfully applied to determine BPA in plastic products and the recovery was in the range from 95.36% to 104.39%. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/20103117/Amperometric_biosensor_based_on_tyrosinase_immobilized_onto_multiwalled_carbon_nanotubes_cobalt_phthalocyanine_silk_fibroin_film_and_its_application_to_determine_bisphenol_A_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(09)01562-1 DB - PRIME DP - Unbound Medicine ER -