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Mutagenicity evaluation of metal oxide nanoparticles by the bacterial reverse mutation assay.
Chemosphere. 2010 Mar; 79(1):113-6.C

Abstract

Nanomaterials have been emerging as a new group of contaminants in the environment. We reported the use of a bacterial reverse mutation assay (Ames assay) to evaluate the mutagenicity of five metal oxide nanoparticles Al(2)O(3), Co(3)O(4), CuO, TiO(2), and ZnO in this study. Results showed the mutagenicity was negative for four nanoparticles (Al(2)O(3), Co(3)O(4), TiO(2), and ZnO) up to 1000mug/plate to all three tested strains without S9 metabolic activation. Using a preincubation procedure and high S9 (9%) activation, TiO(2) and ZnO induced marginal mutagenesis to strain Escherichia coli WP2 trp uvrA. CuO displayed low mutagenic potential to Salmonella typhimurium TA97a and TA100 at specific concentrations. However, the colony inhibition effect of CuO was predominant to the strain E. coli WP2 trp uvrA. A dose-dependent inhibition of Escherichia coli WP2 colony was found under CuO exposure at concentration range of 100-1600mug/plate. No growth inhibition of tested bacterial strains by Al(2)O(3), Co(3)O(4), and ZnO was observed at the concentrations used.

Authors+Show Affiliations

Department of Chemistry, Western Illinois University, Macomb, IL 61455, USA; Department of Biology, East Carolina University, Greenville, NC 27858, USA. Panx@ecu.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20106502

Citation

Pan, Xiaoping, et al. "Mutagenicity Evaluation of Metal Oxide Nanoparticles By the Bacterial Reverse Mutation Assay." Chemosphere, vol. 79, no. 1, 2010, pp. 113-6.
Pan X, Redding JE, Wiley PA, et al. Mutagenicity evaluation of metal oxide nanoparticles by the bacterial reverse mutation assay. Chemosphere. 2010;79(1):113-6.
Pan, X., Redding, J. E., Wiley, P. A., Wen, L., McConnell, J. S., & Zhang, B. (2010). Mutagenicity evaluation of metal oxide nanoparticles by the bacterial reverse mutation assay. Chemosphere, 79(1), 113-6. https://doi.org/10.1016/j.chemosphere.2009.12.056
Pan X, et al. Mutagenicity Evaluation of Metal Oxide Nanoparticles By the Bacterial Reverse Mutation Assay. Chemosphere. 2010;79(1):113-6. PubMed PMID: 20106502.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mutagenicity evaluation of metal oxide nanoparticles by the bacterial reverse mutation assay. AU - Pan,Xiaoping, AU - Redding,James E, AU - Wiley,Patricia A, AU - Wen,Lisa, AU - McConnell,J Scott, AU - Zhang,Baohong, Y1 - 2010/01/27/ PY - 2009/11/08/received PY - 2009/12/13/revised PY - 2009/12/21/accepted PY - 2010/1/29/entrez PY - 2010/1/29/pubmed PY - 2010/6/15/medline SP - 113 EP - 6 JF - Chemosphere JO - Chemosphere VL - 79 IS - 1 N2 - Nanomaterials have been emerging as a new group of contaminants in the environment. We reported the use of a bacterial reverse mutation assay (Ames assay) to evaluate the mutagenicity of five metal oxide nanoparticles Al(2)O(3), Co(3)O(4), CuO, TiO(2), and ZnO in this study. Results showed the mutagenicity was negative for four nanoparticles (Al(2)O(3), Co(3)O(4), TiO(2), and ZnO) up to 1000mug/plate to all three tested strains without S9 metabolic activation. Using a preincubation procedure and high S9 (9%) activation, TiO(2) and ZnO induced marginal mutagenesis to strain Escherichia coli WP2 trp uvrA. CuO displayed low mutagenic potential to Salmonella typhimurium TA97a and TA100 at specific concentrations. However, the colony inhibition effect of CuO was predominant to the strain E. coli WP2 trp uvrA. A dose-dependent inhibition of Escherichia coli WP2 colony was found under CuO exposure at concentration range of 100-1600mug/plate. No growth inhibition of tested bacterial strains by Al(2)O(3), Co(3)O(4), and ZnO was observed at the concentrations used. SN - 1879-1298 UR - https://www.unboundmedicine.com/medline/citation/20106502/Mutagenicity_evaluation_of_metal_oxide_nanoparticles_by_the_bacterial_reverse_mutation_assay_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0045-6535(09)01479-9 DB - PRIME DP - Unbound Medicine ER -