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Apolipoprotein E genotype is associated with macular pigment optical density.
Invest Ophthalmol Vis Sci. 2010 May; 51(5):2636-43.IO

Abstract

PURPOSE

Age-related macular degeneration (AMD) is the most common cause of blindness in older people in developed countries, and risk factors for this condition may be classified as genetic and environmental. Apolipoprotein E is putatively involved in the transport of the macular pigment (MP) carotenoids lutein (L) and zeaxanthin (Z) in serum and may also influence retinal capture of these compounds. This study was designed to investigate the relationship between macular pigment optical density (MPOD) and ApoE genotype.

METHODS

This was a cross-sectional study of 302 healthy adult subjects. Dietary intake of L and Z was assessed by food frequency questionnaire, and MPOD was measured by customized heterochromatic flicker photometry. Serum L and Z were measured by HPLC. ApoE genotyping was performed by direct polymerase chain reaction amplification and DNA nucleotide sequencing from peripheral blood.

RESULTS

Genotype data were available on 300 of the 302 (99.3%) subjects. The mean (+/- SD) age of the subjects in this study was 47.89 +/- 11.05 (range, 21-66) years. Subjects were classed into one of three ApoE genotype groups, as follows: group 1, epsilon2epsilon2 or epsilon2epsilon3; group 2, epsilon3epsilon3; group 3, epsilon2epsilon4 or epsilon3epsilon4 or epsilon4epsilon4. All three groups were statistically comparable in terms of age, sex, body mass index, cigarette smoking, and dietary and serum levels of L and Z. There was a statistically significant association between ApoE genotype and MPOD. Subjects who had at least one epsilon4 allele had a higher MPOD across the macula than subjects without this allele (group 1 MPOD area, 0.70 +/- 0.40; group 2 MPOD area, 0.67 +/- 0.42; group 3 MPOD area, 0.85 +/- 0.46; one-way ANOVA, P = 0.014.

CONCLUSIONS

These results suggest that ApoE genotype status is associated with MPOD. This association may explain, at least in part, the putative protective effect of the epsilon4 allele for AMD and is consistent with the view that apolipoprotein profile influences the transport and/or retinal capture of circulating L and/or Z.

Authors+Show Affiliations

Department of Chemical and Life Sciences, Waterford Institute of Technology, Waterford, Ireland. edwardloane@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20107178

Citation

Loane, Edward, et al. "Apolipoprotein E Genotype Is Associated With Macular Pigment Optical Density." Investigative Ophthalmology & Visual Science, vol. 51, no. 5, 2010, pp. 2636-43.
Loane E, McKay GJ, Nolan JM, et al. Apolipoprotein E genotype is associated with macular pigment optical density. Invest Ophthalmol Vis Sci. 2010;51(5):2636-43.
Loane, E., McKay, G. J., Nolan, J. M., & Beatty, S. (2010). Apolipoprotein E genotype is associated with macular pigment optical density. Investigative Ophthalmology & Visual Science, 51(5), 2636-43. https://doi.org/10.1167/iovs.09-4397
Loane E, et al. Apolipoprotein E Genotype Is Associated With Macular Pigment Optical Density. Invest Ophthalmol Vis Sci. 2010;51(5):2636-43. PubMed PMID: 20107178.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Apolipoprotein E genotype is associated with macular pigment optical density. AU - Loane,Edward, AU - McKay,Gareth J, AU - Nolan,John M, AU - Beatty,Stephen, Y1 - 2010/01/27/ PY - 2010/1/29/entrez PY - 2010/1/29/pubmed PY - 2010/5/21/medline SP - 2636 EP - 43 JF - Investigative ophthalmology & visual science JO - Invest Ophthalmol Vis Sci VL - 51 IS - 5 N2 - PURPOSE: Age-related macular degeneration (AMD) is the most common cause of blindness in older people in developed countries, and risk factors for this condition may be classified as genetic and environmental. Apolipoprotein E is putatively involved in the transport of the macular pigment (MP) carotenoids lutein (L) and zeaxanthin (Z) in serum and may also influence retinal capture of these compounds. This study was designed to investigate the relationship between macular pigment optical density (MPOD) and ApoE genotype. METHODS: This was a cross-sectional study of 302 healthy adult subjects. Dietary intake of L and Z was assessed by food frequency questionnaire, and MPOD was measured by customized heterochromatic flicker photometry. Serum L and Z were measured by HPLC. ApoE genotyping was performed by direct polymerase chain reaction amplification and DNA nucleotide sequencing from peripheral blood. RESULTS: Genotype data were available on 300 of the 302 (99.3%) subjects. The mean (+/- SD) age of the subjects in this study was 47.89 +/- 11.05 (range, 21-66) years. Subjects were classed into one of three ApoE genotype groups, as follows: group 1, epsilon2epsilon2 or epsilon2epsilon3; group 2, epsilon3epsilon3; group 3, epsilon2epsilon4 or epsilon3epsilon4 or epsilon4epsilon4. All three groups were statistically comparable in terms of age, sex, body mass index, cigarette smoking, and dietary and serum levels of L and Z. There was a statistically significant association between ApoE genotype and MPOD. Subjects who had at least one epsilon4 allele had a higher MPOD across the macula than subjects without this allele (group 1 MPOD area, 0.70 +/- 0.40; group 2 MPOD area, 0.67 +/- 0.42; group 3 MPOD area, 0.85 +/- 0.46; one-way ANOVA, P = 0.014. CONCLUSIONS: These results suggest that ApoE genotype status is associated with MPOD. This association may explain, at least in part, the putative protective effect of the epsilon4 allele for AMD and is consistent with the view that apolipoprotein profile influences the transport and/or retinal capture of circulating L and/or Z. SN - 1552-5783 UR - https://www.unboundmedicine.com/medline/citation/20107178/Apolipoprotein_E_genotype_is_associated_with_macular_pigment_optical_density_ L2 - https://iovs.arvojournals.org/article.aspx?doi=10.1167/iovs.09-4397 DB - PRIME DP - Unbound Medicine ER -