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Human langerhans cells are more efficient than CD14(-)CD1c(+) dermal dendritic cells at priming naive CD4(+) T cells.
J Invest Dermatol. 2010 May; 130(5):1345-54.JI

Abstract

Few data are available regarding the role of human skin dendritic cells (DCs) in driving T-cell responses. In this study we analyzed the relative capacity of Langerhans cells (LCs) and dermal CD14(-)CD1c(+) DCs (DDCs) to trigger naive CD4(+) T-cell proliferation and differentiation. DC subsets were purified after a 2-day migration from epidermis and dermis of the same skin sample. Migratory LCs showed far more activated phenotype than CD1c(+)DDCs and distinct expression of new molecules of the B7 family; when compared with LCs, CD1c(+)DDCs showed higher PD-L1 and lower inducible co-stimulator ligand (ICOS-L) expression. As expected, CD1c(+)DDCs showed lower allostimulatory property than LCs, a process that was partly reversed by anti-PD-L1 mAb. LCs were significantly more efficient than CD1c(+)DDCs at inducing allogeneic naive CD4(+) T cells to secrete both T helper cell 1 (Th1; IFN-gamma and tumor necrosis factor-alpha) and Th2 (IL-4 and IL-5) cytokines. Moreover, anti-PD-L1 mAb increased the production of IFN-gamma by both LC- and CD1c(+)DDC-stimulated T cells. Globally, these results argue for a preponderant role of human LCs in inducing naive CD4(+) T-cell priming. Low expression of co-stimulatory molecules together with high expression of PD-L1 might limit the efficiency of CD1c(+)DDCs at inducing naive CD4(+) T-cell proliferation and secretion of cytokines.

Authors+Show Affiliations

Clinique Dermatologique, Université de Lyon, Hopital E. Herriot, Lyon, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

20107482

Citation

Furio, Laetitia, et al. "Human Langerhans Cells Are More Efficient Than CD14(-)CD1c(+) Dermal Dendritic Cells at Priming Naive CD4(+) T Cells." The Journal of Investigative Dermatology, vol. 130, no. 5, 2010, pp. 1345-54.
Furio L, Briotet I, Journeaux A, et al. Human langerhans cells are more efficient than CD14(-)CD1c(+) dermal dendritic cells at priming naive CD4(+) T cells. J Invest Dermatol. 2010;130(5):1345-54.
Furio, L., Briotet, I., Journeaux, A., Billard, H., & Péguet-Navarro, J. (2010). Human langerhans cells are more efficient than CD14(-)CD1c(+) dermal dendritic cells at priming naive CD4(+) T cells. The Journal of Investigative Dermatology, 130(5), 1345-54. https://doi.org/10.1038/jid.2009.424
Furio L, et al. Human Langerhans Cells Are More Efficient Than CD14(-)CD1c(+) Dermal Dendritic Cells at Priming Naive CD4(+) T Cells. J Invest Dermatol. 2010;130(5):1345-54. PubMed PMID: 20107482.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human langerhans cells are more efficient than CD14(-)CD1c(+) dermal dendritic cells at priming naive CD4(+) T cells. AU - Furio,Laetitia, AU - Briotet,Isabelle, AU - Journeaux,Alexandra, AU - Billard,Hermine, AU - Péguet-Navarro,Josette, Y1 - 2010/01/28/ PY - 2010/1/29/entrez PY - 2010/1/29/pubmed PY - 2010/5/14/medline SP - 1345 EP - 54 JF - The Journal of investigative dermatology JO - J Invest Dermatol VL - 130 IS - 5 N2 - Few data are available regarding the role of human skin dendritic cells (DCs) in driving T-cell responses. In this study we analyzed the relative capacity of Langerhans cells (LCs) and dermal CD14(-)CD1c(+) DCs (DDCs) to trigger naive CD4(+) T-cell proliferation and differentiation. DC subsets were purified after a 2-day migration from epidermis and dermis of the same skin sample. Migratory LCs showed far more activated phenotype than CD1c(+)DDCs and distinct expression of new molecules of the B7 family; when compared with LCs, CD1c(+)DDCs showed higher PD-L1 and lower inducible co-stimulator ligand (ICOS-L) expression. As expected, CD1c(+)DDCs showed lower allostimulatory property than LCs, a process that was partly reversed by anti-PD-L1 mAb. LCs were significantly more efficient than CD1c(+)DDCs at inducing allogeneic naive CD4(+) T cells to secrete both T helper cell 1 (Th1; IFN-gamma and tumor necrosis factor-alpha) and Th2 (IL-4 and IL-5) cytokines. Moreover, anti-PD-L1 mAb increased the production of IFN-gamma by both LC- and CD1c(+)DDC-stimulated T cells. Globally, these results argue for a preponderant role of human LCs in inducing naive CD4(+) T-cell priming. Low expression of co-stimulatory molecules together with high expression of PD-L1 might limit the efficiency of CD1c(+)DDCs at inducing naive CD4(+) T-cell proliferation and secretion of cytokines. SN - 1523-1747 UR - https://www.unboundmedicine.com/medline/citation/20107482/Human_langerhans_cells_are_more_efficient_than_CD14___CD1c_+__dermal_dendritic_cells_at_priming_naive_CD4_+__T_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-202X(15)34805-3 DB - PRIME DP - Unbound Medicine ER -