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Substituted pyrazinecarboxamides as abiotic elicitors of flavolignan production in Silybum marianum (L.) gaertn cultures in vitro.
Molecules 2010; 15(1):331-40M

Abstract

Substituted pyrazinecarboxamides markedly influenced production of flavonolignans in Silybum marianum callus and suspension cultures. In this study the effect of two compounds, N-(3-iodo-4-methylphenyl)pyrazine-2-carboxamide (1) and N-(3-iodo-4-methylphenyl)-5-tert-butyl-pyrazine-2-carboxamide (2), as abiotic elicitors on flavono-lignan production in callus culture of S. marianum was investigated. Silymarin complex compounds have hepatoprotective, anticancer and also hypocholesterolemic activity. In vitro flavonolignan concentration in cells is very low and the elicitation is one of the methods to increase production. Elicitors were tested at three concentrations and at different culture times. In the case of elicitation with 1, the greatest increase of flavonolignan and taxifoline production was observed at concentration c(1a) after 6-hours of elicitation and after 24 and 72-hours at concentration c(1b). However, increased production of silychristin, one of the compounds in the silymarin complex, was achieved after only 6-hours elicitation with c(1a) (2.95 x 10(-4) mol/L). The content of silychristin was 2-times higher compared to the control sample. An increased production of silychristin was reached with compound 2 at the concentration c(2) (2.53 x 10(-3) mol/L) after 72 h of elicitation. The production of silychristin in this case was increased 12-times compared to control.

Authors+Show Affiliations

Faculty of Pharmacy in Hradec Králové, Charles University in Prague, Heyrovského 1203, 500 05 Hradec Králové, Czech Republic. lenka.tumova@faf.cuni.czNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20110894

Citation

Tůmová, Lenka, et al. "Substituted Pyrazinecarboxamides as Abiotic Elicitors of Flavolignan Production in Silybum Marianum (L.) Gaertn Cultures in Vitro." Molecules (Basel, Switzerland), vol. 15, no. 1, 2010, pp. 331-40.
Tůmová L, Tůma J, Megusar K, et al. Substituted pyrazinecarboxamides as abiotic elicitors of flavolignan production in Silybum marianum (L.) gaertn cultures in vitro. Molecules. 2010;15(1):331-40.
Tůmová, L., Tůma, J., Megusar, K., & Dolezal, M. (2010). Substituted pyrazinecarboxamides as abiotic elicitors of flavolignan production in Silybum marianum (L.) gaertn cultures in vitro. Molecules (Basel, Switzerland), 15(1), pp. 331-40. doi:10.3390/molecules15010331.
Tůmová L, et al. Substituted Pyrazinecarboxamides as Abiotic Elicitors of Flavolignan Production in Silybum Marianum (L.) Gaertn Cultures in Vitro. Molecules. 2010 Jan 14;15(1):331-40. PubMed PMID: 20110894.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Substituted pyrazinecarboxamides as abiotic elicitors of flavolignan production in Silybum marianum (L.) gaertn cultures in vitro. AU - Tůmová,Lenka, AU - Tůma,Jirí, AU - Megusar,Klara, AU - Dolezal,Martin, Y1 - 2010/01/14/ PY - 2009/11/27/received PY - 2010/01/11/revised PY - 2010/01/12/accepted PY - 2010/1/30/entrez PY - 2010/1/30/pubmed PY - 2010/4/3/medline SP - 331 EP - 40 JF - Molecules (Basel, Switzerland) JO - Molecules VL - 15 IS - 1 N2 - Substituted pyrazinecarboxamides markedly influenced production of flavonolignans in Silybum marianum callus and suspension cultures. In this study the effect of two compounds, N-(3-iodo-4-methylphenyl)pyrazine-2-carboxamide (1) and N-(3-iodo-4-methylphenyl)-5-tert-butyl-pyrazine-2-carboxamide (2), as abiotic elicitors on flavono-lignan production in callus culture of S. marianum was investigated. Silymarin complex compounds have hepatoprotective, anticancer and also hypocholesterolemic activity. In vitro flavonolignan concentration in cells is very low and the elicitation is one of the methods to increase production. Elicitors were tested at three concentrations and at different culture times. In the case of elicitation with 1, the greatest increase of flavonolignan and taxifoline production was observed at concentration c(1a) after 6-hours of elicitation and after 24 and 72-hours at concentration c(1b). However, increased production of silychristin, one of the compounds in the silymarin complex, was achieved after only 6-hours elicitation with c(1a) (2.95 x 10(-4) mol/L). The content of silychristin was 2-times higher compared to the control sample. An increased production of silychristin was reached with compound 2 at the concentration c(2) (2.53 x 10(-3) mol/L) after 72 h of elicitation. The production of silychristin in this case was increased 12-times compared to control. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/20110894/Substituted_pyrazinecarboxamides_as_abiotic_elicitors_of_flavolignan_production_in_Silybum_marianum__L___gaertn_cultures_in_vitro_ L2 - http://www.mdpi.com/resolver?pii=15010331 DB - PRIME DP - Unbound Medicine ER -