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Expression of OCTN2 and OCTN3 in the apical membrane of rat renal cortex and medulla.
J Cell Physiol. 2010 May; 223(2):451-9.JC

Abstract

Immunological assays and transport measurements in apical membrane vesicles revealed that the apical membrane of rat kidney cortex and medulla presents OCTN2 and OCTN3 proteins and transports L-[(3)H]-carnitine in a Na(+)-dependent and -independent manner. OCTN2 mediates the Na(+)/L-carnitine transport activity measured in medulla because (i) the transport showed the same characteristics as the cortical Na(+)/L-carnitine transporter and (ii) the medulla expressed OCTN2 mRNA and protein. The Na(+)-independent L-carnitine transport activity appears to be mediated by both OCTN2 and OCTN3 since: (i) Na(+)-independent L-carnitine uptake was inhibited by both, anti-OCTN2 and anti-OCTN3 antibodies, (ii) kinetics studies revealed the involvement of a high- and a low-affinity transport systems, and (iii) Western and immunohistochemistry studies revealed that OCTN3 protein is located at the apical membrane of the kidney epithelia. The Na(+)-independent L-carnitine uptake exhibited trans-stimulation by intravesicular L-carnitine or betaine. This trans-stimulation was inhibited by anti-OCTN3 antibody, but not by anti-OCTN2 antibody, indicating that OCTN3 can function as an L-carnitine/organic compound exchanger. This is the first report showing a functional apical OCTN2 in the renal medulla and a functional apical OCTN3 in both renal cortex and medulla.

Authors+Show Affiliations

Facultad de Farmacia, Departamento de Fisiología y Zoología (Biomembranes Group), Universidad de Sevilla, 41012Sevilla, Spain.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20112288

Citation

Cano, M M., et al. "Expression of OCTN2 and OCTN3 in the Apical Membrane of Rat Renal Cortex and Medulla." Journal of Cellular Physiology, vol. 223, no. 2, 2010, pp. 451-9.
Cano MM, Calonge ML, Ilundain AA. Expression of OCTN2 and OCTN3 in the apical membrane of rat renal cortex and medulla. J Cell Physiol. 2010;223(2):451-9.
Cano, M. M., Calonge, M. L., & Ilundain, A. A. (2010). Expression of OCTN2 and OCTN3 in the apical membrane of rat renal cortex and medulla. Journal of Cellular Physiology, 223(2), 451-9. https://doi.org/10.1002/jcp.22054
Cano MM, Calonge ML, Ilundain AA. Expression of OCTN2 and OCTN3 in the Apical Membrane of Rat Renal Cortex and Medulla. J Cell Physiol. 2010;223(2):451-9. PubMed PMID: 20112288.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of OCTN2 and OCTN3 in the apical membrane of rat renal cortex and medulla. AU - Cano,M M, AU - Calonge,M L, AU - Ilundain,A A, PY - 2010/1/30/entrez PY - 2010/1/30/pubmed PY - 2010/5/7/medline SP - 451 EP - 9 JF - Journal of cellular physiology JO - J Cell Physiol VL - 223 IS - 2 N2 - Immunological assays and transport measurements in apical membrane vesicles revealed that the apical membrane of rat kidney cortex and medulla presents OCTN2 and OCTN3 proteins and transports L-[(3)H]-carnitine in a Na(+)-dependent and -independent manner. OCTN2 mediates the Na(+)/L-carnitine transport activity measured in medulla because (i) the transport showed the same characteristics as the cortical Na(+)/L-carnitine transporter and (ii) the medulla expressed OCTN2 mRNA and protein. The Na(+)-independent L-carnitine transport activity appears to be mediated by both OCTN2 and OCTN3 since: (i) Na(+)-independent L-carnitine uptake was inhibited by both, anti-OCTN2 and anti-OCTN3 antibodies, (ii) kinetics studies revealed the involvement of a high- and a low-affinity transport systems, and (iii) Western and immunohistochemistry studies revealed that OCTN3 protein is located at the apical membrane of the kidney epithelia. The Na(+)-independent L-carnitine uptake exhibited trans-stimulation by intravesicular L-carnitine or betaine. This trans-stimulation was inhibited by anti-OCTN3 antibody, but not by anti-OCTN2 antibody, indicating that OCTN3 can function as an L-carnitine/organic compound exchanger. This is the first report showing a functional apical OCTN2 in the renal medulla and a functional apical OCTN3 in both renal cortex and medulla. SN - 1097-4652 UR - https://www.unboundmedicine.com/medline/citation/20112288/Expression_of_OCTN2_and_OCTN3_in_the_apical_membrane_of_rat_renal_cortex_and_medulla_ L2 - https://doi.org/10.1002/jcp.22054 DB - PRIME DP - Unbound Medicine ER -