Tags

Type your tag names separated by a space and hit enter

Myogenic skeletal muscle satellite cells communicate by tunnelling nanotubes.
J Cell Physiol. 2010 May; 223(2):376-83.JC

Abstract

Quiescent satellite cells sit on the surface of the muscle fibres under the basal lamina and are activated by a variety of stimuli to disengage, divide and differentiate into myoblasts that can regenerate or repair muscle fibres. Satellite cells adopt their parent's fibre type and must have some means of communication with the parent fibre. The mechanisms behind this communication are not known. We show here that satellite cells form dynamic connections with muscle fibres and other satellite cells by F-actin based tunnelling nanotubes (TNTs). Our results show that TNTs readily develop between satellite cells and muscle fibres. Once developed, TNTs permit transport of intracellular material, and even cellular organelles such as mitochondria between the muscle fibre and satellite cells. The onset of satellite cell differentiation markers Pax-7 and MyoD expression was slower in satellite cells cultured in the absence than in the presence of muscle cells. Furthermore physical contact between myofibre and satellite cell progeny is required to maintain subtype identity. Our data establish that TNTs constitute an integral part of myogenic cell communication and that physical cellular interaction control myogenic cell fate determination.

Authors+Show Affiliations

Department of Biotechnology and Molecular Medicine, A. I. Virtanen Institute for Molecular Medicine, University of Kuopio, FIN-70211 Kuopio, Finland. pasi.tavi@uku.fiNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20112291

Citation

Tavi, Pasi, et al. "Myogenic Skeletal Muscle Satellite Cells Communicate By Tunnelling Nanotubes." Journal of Cellular Physiology, vol. 223, no. 2, 2010, pp. 376-83.
Tavi P, Korhonen T, Hänninen SL, et al. Myogenic skeletal muscle satellite cells communicate by tunnelling nanotubes. J Cell Physiol. 2010;223(2):376-83.
Tavi, P., Korhonen, T., Hänninen, S. L., Bruton, J. D., Lööf, S., Simon, A., & Westerblad, H. (2010). Myogenic skeletal muscle satellite cells communicate by tunnelling nanotubes. Journal of Cellular Physiology, 223(2), 376-83. https://doi.org/10.1002/jcp.22044
Tavi P, et al. Myogenic Skeletal Muscle Satellite Cells Communicate By Tunnelling Nanotubes. J Cell Physiol. 2010;223(2):376-83. PubMed PMID: 20112291.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Myogenic skeletal muscle satellite cells communicate by tunnelling nanotubes. AU - Tavi,Pasi, AU - Korhonen,Topi, AU - Hänninen,Sandra L, AU - Bruton,Joseph D, AU - Lööf,Sara, AU - Simon,Andras, AU - Westerblad,Håkan, PY - 2010/1/30/entrez PY - 2010/1/30/pubmed PY - 2010/5/7/medline SP - 376 EP - 83 JF - Journal of cellular physiology JO - J Cell Physiol VL - 223 IS - 2 N2 - Quiescent satellite cells sit on the surface of the muscle fibres under the basal lamina and are activated by a variety of stimuli to disengage, divide and differentiate into myoblasts that can regenerate or repair muscle fibres. Satellite cells adopt their parent's fibre type and must have some means of communication with the parent fibre. The mechanisms behind this communication are not known. We show here that satellite cells form dynamic connections with muscle fibres and other satellite cells by F-actin based tunnelling nanotubes (TNTs). Our results show that TNTs readily develop between satellite cells and muscle fibres. Once developed, TNTs permit transport of intracellular material, and even cellular organelles such as mitochondria between the muscle fibre and satellite cells. The onset of satellite cell differentiation markers Pax-7 and MyoD expression was slower in satellite cells cultured in the absence than in the presence of muscle cells. Furthermore physical contact between myofibre and satellite cell progeny is required to maintain subtype identity. Our data establish that TNTs constitute an integral part of myogenic cell communication and that physical cellular interaction control myogenic cell fate determination. SN - 1097-4652 UR - https://www.unboundmedicine.com/medline/citation/20112291/Myogenic_skeletal_muscle_satellite_cells_communicate_by_tunnelling_nanotubes_ L2 - https://doi.org/10.1002/jcp.22044 DB - PRIME DP - Unbound Medicine ER -