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Genetic diversity of Salmonella enteric serovar typhi and paratyphi in Shenzhen, China from 2002 through 2007.
BMC Microbiol. 2010 Jan 30; 10:32.BM

Abstract

BACKGROUND

Typhoid and paratyphoid fever are endemic in China. The objective of this investigation was to determine the molecular features of nalidixic acid-resistant Salmonella enteric serovar Typhi (S. typhi) and Paratyphi (S. paratyphi) from blood isolates in Shenzhen, China.

RESULTS

Twenty-five S. typhi and 66 S. paratyphi were isolated from 91 bacteremic patients between 2002 and 2007 at a hospital in Shenzhen, Southern China. Fifty-two percent (13/25) of S. typhi and 95.3% (61/64) of S. paratyphi A were resistant to nalidixic acid. Sixty-seven isolates of nalidixic acid-resistant Salmonella (NARS) showed decreased susceptibility to ciprofloxacin (MICs of 0.125-1 microg/mL). All 75 NARS isolates had a single substitution in the quinolone resistance-determining region (QRDR) of GyrA (Ser83-->Phe/Pro/Tyr, or Asp87-->Gly/Asn), and 90.7% of these isolates carried the substitution Ser83Phe in GyrA. No mutation was found in the QRDR of gyrB, parC, or parE. Plasmid mediated quinolone resistance genes including qnr and aac(6')-Ib-cr were not detected in any isolate. Twenty-two distinct pulsed field gel electrophoresis (PFGE) patterns were observed among S. typhi. Sixty-four isolates of S. paratyphi A belonged to one clone. Eighty-seven investigated inpatients were infected in the community. Six patients infected by S. paratyphi A had a travel history before infection.

CONCLUSIONS

Nalidixic acid-resistant S. typhi and S. paratyphi A blood isolates were highly prevalent in Shenzhen, China. PFGE showed the variable genetic diversity of nalidixic acid-resistant S. typhi and limited genetic diversity of nalidixic acid -resistant S. paratyphi A.

Authors+Show Affiliations

Department of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20113512

Citation

Wu, Weiyuan, et al. "Genetic Diversity of Salmonella Enteric Serovar Typhi and Paratyphi in Shenzhen, China From 2002 Through 2007." BMC Microbiology, vol. 10, 2010, p. 32.
Wu W, Wang H, Lu J, et al. Genetic diversity of Salmonella enteric serovar typhi and paratyphi in Shenzhen, China from 2002 through 2007. BMC Microbiol. 2010;10:32.
Wu, W., Wang, H., Lu, J., Wu, J., Chen, M., Xu, Y., & Lu, Y. (2010). Genetic diversity of Salmonella enteric serovar typhi and paratyphi in Shenzhen, China from 2002 through 2007. BMC Microbiology, 10, 32. https://doi.org/10.1186/1471-2180-10-32
Wu W, et al. Genetic Diversity of Salmonella Enteric Serovar Typhi and Paratyphi in Shenzhen, China From 2002 Through 2007. BMC Microbiol. 2010 Jan 30;10:32. PubMed PMID: 20113512.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic diversity of Salmonella enteric serovar typhi and paratyphi in Shenzhen, China from 2002 through 2007. AU - Wu,Weiyuan, AU - Wang,Hui, AU - Lu,Jian, AU - Wu,Jinsong, AU - Chen,Minjun, AU - Xu,Yingchun, AU - Lu,Yuemei, Y1 - 2010/01/30/ PY - 2009/09/20/received PY - 2010/01/30/accepted PY - 2010/2/2/entrez PY - 2010/2/2/pubmed PY - 2010/3/17/medline SP - 32 EP - 32 JF - BMC microbiology JO - BMC Microbiol VL - 10 N2 - BACKGROUND: Typhoid and paratyphoid fever are endemic in China. The objective of this investigation was to determine the molecular features of nalidixic acid-resistant Salmonella enteric serovar Typhi (S. typhi) and Paratyphi (S. paratyphi) from blood isolates in Shenzhen, China. RESULTS: Twenty-five S. typhi and 66 S. paratyphi were isolated from 91 bacteremic patients between 2002 and 2007 at a hospital in Shenzhen, Southern China. Fifty-two percent (13/25) of S. typhi and 95.3% (61/64) of S. paratyphi A were resistant to nalidixic acid. Sixty-seven isolates of nalidixic acid-resistant Salmonella (NARS) showed decreased susceptibility to ciprofloxacin (MICs of 0.125-1 microg/mL). All 75 NARS isolates had a single substitution in the quinolone resistance-determining region (QRDR) of GyrA (Ser83-->Phe/Pro/Tyr, or Asp87-->Gly/Asn), and 90.7% of these isolates carried the substitution Ser83Phe in GyrA. No mutation was found in the QRDR of gyrB, parC, or parE. Plasmid mediated quinolone resistance genes including qnr and aac(6')-Ib-cr were not detected in any isolate. Twenty-two distinct pulsed field gel electrophoresis (PFGE) patterns were observed among S. typhi. Sixty-four isolates of S. paratyphi A belonged to one clone. Eighty-seven investigated inpatients were infected in the community. Six patients infected by S. paratyphi A had a travel history before infection. CONCLUSIONS: Nalidixic acid-resistant S. typhi and S. paratyphi A blood isolates were highly prevalent in Shenzhen, China. PFGE showed the variable genetic diversity of nalidixic acid-resistant S. typhi and limited genetic diversity of nalidixic acid -resistant S. paratyphi A. SN - 1471-2180 UR - https://www.unboundmedicine.com/medline/citation/20113512/Genetic_diversity_of_Salmonella_enteric_serovar_typhi_and_paratyphi_in_Shenzhen_China_from_2002_through_2007_ L2 - https://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-10-32 DB - PRIME DP - Unbound Medicine ER -