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[Effects of prolonged exposure of high concentration of oxygen on expression of vascular endothelial growth factor and its receptors in neonatal rat lungs].
Zhongguo Dang Dai Er Ke Za Zhi. 2009 Nov; 11(11):927-30.ZD

Abstract

OBJECTIVE

To study the effects of prolonged 75% oxygen exposure on the expression of vascular endothelial growth factor (VEGF) and its receptors (VEGFR1 and VEGFR2) in the neonatal rat lungs and to elucidate the effects of prolonged exposure of high concentration of oxygen on lung vascular development and its relationship with bronchopulmonary dysplasia (BPD).

METHODS

Forty eight Sprague-Dawley rat pups were randomly exposed to air (control group) and 75% oxygen (experimental group) 12 hrs after birth. The rats were sacrificed 7, 14 and 21 days after exposure and their lungs were sampled. The lung sections were stained with hematoxylin and eosin for histological evaluation. Expression of VEGF, VEGFR1 and VEGFR2 protein and mRNA was detected by immunohistochemistry and RT-PCR.

RESULTS

After being exposed to 75% oxygen for 21 days, lung tissues had pathological changes as 'new' BPD. Expressions of VEGF protein (10.9 + or - 2.7 vs 30.8 + or - 6.4), VEGFR1 protein (5.4 + or - 1.4 vs 15.6 + or - 3.4) and VEGFR2 protein (11.3 + or - 2.6 vs 21.7 + or - 4.5) on day 21 in the experimental group decreased significantly as compared with the control group (p<0.05). The expression of VEGF mRNA (1.6 vs 3.3), VEGFR1 mRNA (0.4 vs 6.6) and VEGFR2 mRNA (0.5 vs 4.9) on day 21 in the experimental group also decreased significantly as compared with the control group (p<0.05).

CONCLUSIONS

Prolonged exposure of high concentration of oxygen may cause BPD possibly by inhibiting lung vascular development in neonatal rats.

Authors+Show Affiliations

Children's Hospital of Fudan University, Shanghai 201102, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

20113663

Citation

Feng, Hai-Yan, et al. "[Effects of Prolonged Exposure of High Concentration of Oxygen On Expression of Vascular Endothelial Growth Factor and Its Receptors in Neonatal Rat Lungs]." Zhongguo Dang Dai Er Ke Za Zhi = Chinese Journal of Contemporary Pediatrics, vol. 11, no. 11, 2009, pp. 927-30.
Feng HY, Lu AZ, Zhang XB, et al. [Effects of prolonged exposure of high concentration of oxygen on expression of vascular endothelial growth factor and its receptors in neonatal rat lungs]. Zhongguo Dang Dai Er Ke Za Zhi. 2009;11(11):927-30.
Feng, H. Y., Lu, A. Z., Zhang, X. B., Wang, L. B., & Chen, C. (2009). [Effects of prolonged exposure of high concentration of oxygen on expression of vascular endothelial growth factor and its receptors in neonatal rat lungs]. Zhongguo Dang Dai Er Ke Za Zhi = Chinese Journal of Contemporary Pediatrics, 11(11), 927-30.
Feng HY, et al. [Effects of Prolonged Exposure of High Concentration of Oxygen On Expression of Vascular Endothelial Growth Factor and Its Receptors in Neonatal Rat Lungs]. Zhongguo Dang Dai Er Ke Za Zhi. 2009;11(11):927-30. PubMed PMID: 20113663.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of prolonged exposure of high concentration of oxygen on expression of vascular endothelial growth factor and its receptors in neonatal rat lungs]. AU - Feng,Hai-Yan, AU - Lu,Ai-Zhen, AU - Zhang,Xiao-Bo, AU - Wang,Li-Bo, AU - Chen,Chao, PY - 2010/2/2/entrez PY - 2010/2/2/pubmed PY - 2010/3/3/medline SP - 927 EP - 30 JF - Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics JO - Zhongguo Dang Dai Er Ke Za Zhi VL - 11 IS - 11 N2 - OBJECTIVE: To study the effects of prolonged 75% oxygen exposure on the expression of vascular endothelial growth factor (VEGF) and its receptors (VEGFR1 and VEGFR2) in the neonatal rat lungs and to elucidate the effects of prolonged exposure of high concentration of oxygen on lung vascular development and its relationship with bronchopulmonary dysplasia (BPD). METHODS: Forty eight Sprague-Dawley rat pups were randomly exposed to air (control group) and 75% oxygen (experimental group) 12 hrs after birth. The rats were sacrificed 7, 14 and 21 days after exposure and their lungs were sampled. The lung sections were stained with hematoxylin and eosin for histological evaluation. Expression of VEGF, VEGFR1 and VEGFR2 protein and mRNA was detected by immunohistochemistry and RT-PCR. RESULTS: After being exposed to 75% oxygen for 21 days, lung tissues had pathological changes as 'new' BPD. Expressions of VEGF protein (10.9 + or - 2.7 vs 30.8 + or - 6.4), VEGFR1 protein (5.4 + or - 1.4 vs 15.6 + or - 3.4) and VEGFR2 protein (11.3 + or - 2.6 vs 21.7 + or - 4.5) on day 21 in the experimental group decreased significantly as compared with the control group (p<0.05). The expression of VEGF mRNA (1.6 vs 3.3), VEGFR1 mRNA (0.4 vs 6.6) and VEGFR2 mRNA (0.5 vs 4.9) on day 21 in the experimental group also decreased significantly as compared with the control group (p<0.05). CONCLUSIONS: Prolonged exposure of high concentration of oxygen may cause BPD possibly by inhibiting lung vascular development in neonatal rats. SN - 1008-8830 UR - https://www.unboundmedicine.com/medline/citation/20113663/[Effects_of_prolonged_exposure_of_high_concentration_of_oxygen_on_expression_of_vascular_endothelial_growth_factor_and_its_receptors_in_neonatal_rat_lungs]_ L2 - http://www.zgddek.com/EN/abstract/abstract12107.shtml DB - PRIME DP - Unbound Medicine ER -