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Limnohabitans australis sp. nov., isolated from a freshwater pond, and emended description of the genus Limnohabitans.

Abstract

A chemo-organotrophic, aerobic, non-motile strain, MWH-BRAZ-DAM2D(T), isolated from a freshwater pond in Brazil, was characterized phenotypically, phylogenetically and chemotaxonomically. Phylogenetic analysis of 16S rRNA gene sequences indicated affiliation of the strain with the genus Limnohabitans (Comamonadaceae, Betaproteobacteria). 16S rRNA gene sequence similarities between the isolate and Limnohabitans curvus MWH-C5(T), representing the type species of the genus, and the type strains of Limnohabitans parvus and Limnohabitans planktonicus were 98.2, 96.5 and 97.0 %, respectively. DNA-DNA reassociation analyses with DNA of the type strains of all three previously described Limnohabitans species revealed similarity values in the range 26.2-44.6 %. The predominant fatty acids of the isolate were C(16 : 1)ω7c/ω6c, C(16 : 0), C(12 : 0) and C(8 : 0) 3-OH, the major quinone was ubiquinone Q-8 and the DNA G+C content was 55.8 mol%. The isolate could be discriminated from the type strains of the three Limnohabitans species by several phenotypic traits including differences in the utilization of several carbon sources. Based on the phylogeny of the isolate and its differences from the three most closely related species, the isolate represents a novel species for which the name Limnohabitans australis sp. nov. is proposed. The type strain is MWH-BRAZ-DAM2D(T) (=DSM 21646(T)=CCUG 56719(T)).

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  • Authors+Show Affiliations

    ,

    Institute for Limnology, Austrian Academy of Sciences, Mondseestrasse 9, A-5310 Mondsee, Austria. martin.hahn@oeaw.ac.at

    , , ,

    Source

    MeSH

    Bacterial Typing Techniques
    Base Composition
    Brazil
    Comamonadaceae
    DNA, Bacterial
    Fatty Acids
    Fresh Water
    Molecular Sequence Data
    Nucleic Acid Hybridization
    Phylogeny
    Ponds
    RNA, Ribosomal, 16S
    Sequence Analysis, DNA
    Ubiquinone

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    20118294

    Citation

    TY - JOUR T1 - Limnohabitans australis sp. nov., isolated from a freshwater pond, and emended description of the genus Limnohabitans. AU - Hahn,Martin W, AU - Kasalický,Vojtech, AU - Jezbera,Jan, AU - Brandt,Ulrike, AU - Simek,Karel, Y1 - 2010/01/29/ PY - 2010/1/29/aheadofprint PY - 2010/2/2/entrez PY - 2010/2/2/pubmed PY - 2015/5/7/medline SP - 2946 EP - 50 JF - International journal of systematic and evolutionary microbiology JO - Int. J. Syst. Evol. Microbiol. VL - 60 IS - Pt 12 N2 - A chemo-organotrophic, aerobic, non-motile strain, MWH-BRAZ-DAM2D(T), isolated from a freshwater pond in Brazil, was characterized phenotypically, phylogenetically and chemotaxonomically. Phylogenetic analysis of 16S rRNA gene sequences indicated affiliation of the strain with the genus Limnohabitans (Comamonadaceae, Betaproteobacteria). 16S rRNA gene sequence similarities between the isolate and Limnohabitans curvus MWH-C5(T), representing the type species of the genus, and the type strains of Limnohabitans parvus and Limnohabitans planktonicus were 98.2, 96.5 and 97.0 %, respectively. DNA-DNA reassociation analyses with DNA of the type strains of all three previously described Limnohabitans species revealed similarity values in the range 26.2-44.6 %. The predominant fatty acids of the isolate were C(16 : 1)ω7c/ω6c, C(16 : 0), C(12 : 0) and C(8 : 0) 3-OH, the major quinone was ubiquinone Q-8 and the DNA G+C content was 55.8 mol%. The isolate could be discriminated from the type strains of the three Limnohabitans species by several phenotypic traits including differences in the utilization of several carbon sources. Based on the phylogeny of the isolate and its differences from the three most closely related species, the isolate represents a novel species for which the name Limnohabitans australis sp. nov. is proposed. The type strain is MWH-BRAZ-DAM2D(T) (=DSM 21646(T)=CCUG 56719(T)). SN - 1466-5034 UR - https://www.unboundmedicine.com/medline/citation/20118294/Limnohabitans_australis_sp__nov__isolated_from_a_freshwater_pond_and_emended_description_of_the_genus_Limnohabitans_ L2 - http://ijs.sgmjournals.org/cgi/pmidlookup?view=long&pmid=20118294 ER -