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Chemical analysis and screening as anticancer agent of anthocyanin-rich extract from Uva Caimarona (Pourouma cecropiifolia Mart.) fruit.
J Agric Food Chem. 2010 Feb 24; 58(4):2100-10.JA

Abstract

The anthocyanin-rich extract (ARE) of the fruit from Pourouma cecropiifolia , a tropical plant native to the Amazon region, showed moderate cytotoxicity toward different cancer cell lines when evaluated by MTT assays. This extract was fractionated using Sephadex LH-20 chromatography to obtain three fractions (F1-F3), the composition of which was analyzed by HPLC-PDA and LC-ESI/MS. F1 was composed primarily of the monomeric anthocyanins delphinidin-3-O-beta-glucopyranoside, cyanidin-3-O-beta-glucopyranoside, and cyanidin-3-O-(6''-malonyl)glucopyranoside. F2 contained the isomeric flavonols quercetin 3-O-alpha-rhamnopyranosyl-(1-->6)-beta-galactopyranoside and quercetin 3-O-alpha-rhamnopyranosyl-(1-->6)-beta-glucopyranoside, the structures of which were confirmed by (1)H and (13)C NMR. F3 contained polymeric pigments, which were analyzed using tandem ESI/MS with an ion trap-TOF. The structures of two proanthocyanidin and two flavanol-anthocyanin condensed pigments were suggested on the basis of their MS(n) fragmentation patterns. After cell viability assays were performed, only fraction F3 showed a cell growth-inhibitory effect similar to the one found for ARE. F3 significantly reduced the viability of HEp-2 larynx, MKN-45 gastric carcinoma, and MCF-7 breast cancer cells; in contrast, the pure compounds did not show promising cytotoxicity toward the cancer cells evaluated.

Authors+Show Affiliations

Departamento de Química, Universidad Nacional de Colombia, Bogotá, 41012 Sevilla, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20121190

Citation

Barrios, Juliana, et al. "Chemical Analysis and Screening as Anticancer Agent of Anthocyanin-rich Extract From Uva Caimarona (Pourouma Cecropiifolia Mart.) Fruit." Journal of Agricultural and Food Chemistry, vol. 58, no. 4, 2010, pp. 2100-10.
Barrios J, Cordero CP, Aristizabal F, et al. Chemical analysis and screening as anticancer agent of anthocyanin-rich extract from Uva Caimarona (Pourouma cecropiifolia Mart.) fruit. J Agric Food Chem. 2010;58(4):2100-10.
Barrios, J., Cordero, C. P., Aristizabal, F., Heredia, F. J., Morales, A. L., & Osorio, C. (2010). Chemical analysis and screening as anticancer agent of anthocyanin-rich extract from Uva Caimarona (Pourouma cecropiifolia Mart.) fruit. Journal of Agricultural and Food Chemistry, 58(4), 2100-10. https://doi.org/10.1021/jf9041497
Barrios J, et al. Chemical Analysis and Screening as Anticancer Agent of Anthocyanin-rich Extract From Uva Caimarona (Pourouma Cecropiifolia Mart.) Fruit. J Agric Food Chem. 2010 Feb 24;58(4):2100-10. PubMed PMID: 20121190.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chemical analysis and screening as anticancer agent of anthocyanin-rich extract from Uva Caimarona (Pourouma cecropiifolia Mart.) fruit. AU - Barrios,Juliana, AU - Cordero,Claudia Patricia, AU - Aristizabal,Fabio, AU - Heredia,Francisco José, AU - Morales,Alicia Lucía, AU - Osorio,Coralia, PY - 2010/2/4/entrez PY - 2010/2/4/pubmed PY - 2010/5/29/medline SP - 2100 EP - 10 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 58 IS - 4 N2 - The anthocyanin-rich extract (ARE) of the fruit from Pourouma cecropiifolia , a tropical plant native to the Amazon region, showed moderate cytotoxicity toward different cancer cell lines when evaluated by MTT assays. This extract was fractionated using Sephadex LH-20 chromatography to obtain three fractions (F1-F3), the composition of which was analyzed by HPLC-PDA and LC-ESI/MS. F1 was composed primarily of the monomeric anthocyanins delphinidin-3-O-beta-glucopyranoside, cyanidin-3-O-beta-glucopyranoside, and cyanidin-3-O-(6''-malonyl)glucopyranoside. F2 contained the isomeric flavonols quercetin 3-O-alpha-rhamnopyranosyl-(1-->6)-beta-galactopyranoside and quercetin 3-O-alpha-rhamnopyranosyl-(1-->6)-beta-glucopyranoside, the structures of which were confirmed by (1)H and (13)C NMR. F3 contained polymeric pigments, which were analyzed using tandem ESI/MS with an ion trap-TOF. The structures of two proanthocyanidin and two flavanol-anthocyanin condensed pigments were suggested on the basis of their MS(n) fragmentation patterns. After cell viability assays were performed, only fraction F3 showed a cell growth-inhibitory effect similar to the one found for ARE. F3 significantly reduced the viability of HEp-2 larynx, MKN-45 gastric carcinoma, and MCF-7 breast cancer cells; in contrast, the pure compounds did not show promising cytotoxicity toward the cancer cells evaluated. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/20121190/Chemical_analysis_and_screening_as_anticancer_agent_of_anthocyanin_rich_extract_from_Uva_Caimarona__Pourouma_cecropiifolia_Mart___fruit_ L2 - https://doi.org/10.1021/jf9041497 DB - PRIME DP - Unbound Medicine ER -