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Identification and characterization of anthocyanins in yard-long beans (Vigna unguiculata ssp. sesquipedalis L.) by High-performance liquid chromatography with diode array detection and electrospray ionization/mass spectrometry (HPLC-DAD-ESI/MS) analysis.
J Agric Food Chem. 2010 Feb 24; 58(4):2571-6.JA

Abstract

Anthocyanins play an important role in physiological functions related to human health. The objective of this study was to investigate the profiles of anthocyanins in the immature purple pods and black seeds of yard-long beans (Vigna unguiculata ssp. sesquipedalis L.) using high-performance liquid chromatography (HPLC) with diode array detection and electrospray ionization/mass spectrometry (DAD-ESI/MS) analysis. The individual anthocyanins were identified by comparing their mass spectrometric data and retention times. In the purple pods, five individual anthocyanins were identified: delphinidin-3-O-glucoside (2), cyanidin-3-O-sambubioside (4), cyanidin-3-O-glucoside (5), pelargonidin-3-O-glucoside (7), and peonidin-3-O-glucoside (8). From the black seed coat of the yard-long beans, seven anthocyanins were identified, including delphinidin-3-O-galactoside (1), cyanidin-3-O-galactoside (3), petunidin-3-O-glucoside (6), and malvidin-3-O-glucoside (9), together with compounds 2, 5, and 8. In this study, we report for the first time anthocyanin profiles for the pod and seed coat of yard-long beans.

Authors+Show Affiliations

Department of Functional Crop, National Institute of Crop Science, Rural Development Administration, 1085 Neidong, Miryang 627-803, Korea. taejoung@korea.krNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20121192

Citation

Ha, Tae Joung, et al. "Identification and Characterization of Anthocyanins in Yard-long Beans (Vigna Unguiculata Ssp. Sesquipedalis L.) By High-performance Liquid Chromatography With Diode Array Detection and Electrospray Ionization/mass Spectrometry (HPLC-DAD-ESI/MS) Analysis." Journal of Agricultural and Food Chemistry, vol. 58, no. 4, 2010, pp. 2571-6.
Ha TJ, Lee MH, Park CH, et al. Identification and characterization of anthocyanins in yard-long beans (Vigna unguiculata ssp. sesquipedalis L.) by High-performance liquid chromatography with diode array detection and electrospray ionization/mass spectrometry (HPLC-DAD-ESI/MS) analysis. J Agric Food Chem. 2010;58(4):2571-6.
Ha, T. J., Lee, M. H., Park, C. H., Pae, S. B., Shim, K. B., Ko, J. M., Shin, S. O., Baek, I. Y., & Park, K. Y. (2010). Identification and characterization of anthocyanins in yard-long beans (Vigna unguiculata ssp. sesquipedalis L.) by High-performance liquid chromatography with diode array detection and electrospray ionization/mass spectrometry (HPLC-DAD-ESI/MS) analysis. Journal of Agricultural and Food Chemistry, 58(4), 2571-6. https://doi.org/10.1021/jf903883e
Ha TJ, et al. Identification and Characterization of Anthocyanins in Yard-long Beans (Vigna Unguiculata Ssp. Sesquipedalis L.) By High-performance Liquid Chromatography With Diode Array Detection and Electrospray Ionization/mass Spectrometry (HPLC-DAD-ESI/MS) Analysis. J Agric Food Chem. 2010 Feb 24;58(4):2571-6. PubMed PMID: 20121192.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and characterization of anthocyanins in yard-long beans (Vigna unguiculata ssp. sesquipedalis L.) by High-performance liquid chromatography with diode array detection and electrospray ionization/mass spectrometry (HPLC-DAD-ESI/MS) analysis. AU - Ha,Tae Joung, AU - Lee,Myoung-Hee, AU - Park,Chang-Hwan, AU - Pae,Suk-Bok, AU - Shim,Kang-Bo, AU - Ko,Jong-Min, AU - Shin,Sang-Ouk, AU - Baek,In-Youl, AU - Park,Keum-Yong, PY - 2010/2/4/entrez PY - 2010/2/4/pubmed PY - 2010/5/29/medline SP - 2571 EP - 6 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 58 IS - 4 N2 - Anthocyanins play an important role in physiological functions related to human health. The objective of this study was to investigate the profiles of anthocyanins in the immature purple pods and black seeds of yard-long beans (Vigna unguiculata ssp. sesquipedalis L.) using high-performance liquid chromatography (HPLC) with diode array detection and electrospray ionization/mass spectrometry (DAD-ESI/MS) analysis. The individual anthocyanins were identified by comparing their mass spectrometric data and retention times. In the purple pods, five individual anthocyanins were identified: delphinidin-3-O-glucoside (2), cyanidin-3-O-sambubioside (4), cyanidin-3-O-glucoside (5), pelargonidin-3-O-glucoside (7), and peonidin-3-O-glucoside (8). From the black seed coat of the yard-long beans, seven anthocyanins were identified, including delphinidin-3-O-galactoside (1), cyanidin-3-O-galactoside (3), petunidin-3-O-glucoside (6), and malvidin-3-O-glucoside (9), together with compounds 2, 5, and 8. In this study, we report for the first time anthocyanin profiles for the pod and seed coat of yard-long beans. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/20121192/Identification_and_characterization_of_anthocyanins_in_yard_long_beans__Vigna_unguiculata_ssp__sesquipedalis_L___by_High_performance_liquid_chromatography_with_diode_array_detection_and_electrospray_ionization/mass_spectrometry__HPLC_DAD_ESI/MS__analysis_ L2 - https://doi.org/10.1021/jf903883e DB - PRIME DP - Unbound Medicine ER -