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Signal enhancement of surface enhanced Raman scattering and surface enhanced resonance Raman scattering using in situ colloidal synthesis in microfluidics.
Anal Chem. 2010 Mar 01; 82(5):2119-23.AC

Abstract

We demonstrate the enhanced analytical sensitivity of both surface enhanced Raman scattering (SERS) and surface enhanced resonance Raman scattering (SERRS) responses, resulting from the in situ synthesis of silver colloid in a microfluidic flow structure, where both mixing and optical interrogation were integrated on-chip. The chip-based sensor was characterized with a model Raman active label, rhodamine-6G (R6G), and had a limit of detection (LOD) of ca. 50 fM (equivalent to single molecule detection). The device was also used for the determination of the natural pigment, scytonemin, from cyanobacteria (as an analogue for extraterrestrial life existing in extreme environments). The observed LOD of approximately 10 pM (ca. <400 molecules) demonstrated the analytical advantages of working with freshly synthesized colloid in such a flow system. In both cases, sensitivities were between 1 and 2 orders of magnitude greater in the microfluidic system than those measured using the same experimental parameters, with colloid synthesized off-chip, under quiescent conditions.

Authors+Show Affiliations

Department of Electronics and Electrical Engineering, University of Glasgow, Rankine Building, Oakfield Avenue, Glasgow G12 8LT, UK.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20121214

Citation

Wilson, Rab, et al. "Signal Enhancement of Surface Enhanced Raman Scattering and Surface Enhanced Resonance Raman Scattering Using in Situ Colloidal Synthesis in Microfluidics." Analytical Chemistry, vol. 82, no. 5, 2010, pp. 2119-23.
Wilson R, Bowden SA, Parnell J, et al. Signal enhancement of surface enhanced Raman scattering and surface enhanced resonance Raman scattering using in situ colloidal synthesis in microfluidics. Anal Chem. 2010;82(5):2119-23.
Wilson, R., Bowden, S. A., Parnell, J., & Cooper, J. M. (2010). Signal enhancement of surface enhanced Raman scattering and surface enhanced resonance Raman scattering using in situ colloidal synthesis in microfluidics. Analytical Chemistry, 82(5), 2119-23. https://doi.org/10.1021/ac100060g
Wilson R, et al. Signal Enhancement of Surface Enhanced Raman Scattering and Surface Enhanced Resonance Raman Scattering Using in Situ Colloidal Synthesis in Microfluidics. Anal Chem. 2010 Mar 1;82(5):2119-23. PubMed PMID: 20121214.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Signal enhancement of surface enhanced Raman scattering and surface enhanced resonance Raman scattering using in situ colloidal synthesis in microfluidics. AU - Wilson,Rab, AU - Bowden,Stephen A, AU - Parnell,John, AU - Cooper,Jonathan M, PY - 2010/2/4/entrez PY - 2010/2/4/pubmed PY - 2010/6/15/medline SP - 2119 EP - 23 JF - Analytical chemistry JO - Anal Chem VL - 82 IS - 5 N2 - We demonstrate the enhanced analytical sensitivity of both surface enhanced Raman scattering (SERS) and surface enhanced resonance Raman scattering (SERRS) responses, resulting from the in situ synthesis of silver colloid in a microfluidic flow structure, where both mixing and optical interrogation were integrated on-chip. The chip-based sensor was characterized with a model Raman active label, rhodamine-6G (R6G), and had a limit of detection (LOD) of ca. 50 fM (equivalent to single molecule detection). The device was also used for the determination of the natural pigment, scytonemin, from cyanobacteria (as an analogue for extraterrestrial life existing in extreme environments). The observed LOD of approximately 10 pM (ca. <400 molecules) demonstrated the analytical advantages of working with freshly synthesized colloid in such a flow system. In both cases, sensitivities were between 1 and 2 orders of magnitude greater in the microfluidic system than those measured using the same experimental parameters, with colloid synthesized off-chip, under quiescent conditions. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/20121214/Signal_enhancement_of_surface_enhanced_Raman_scattering_and_surface_enhanced_resonance_Raman_scattering_using_in_situ_colloidal_synthesis_in_microfluidics_ L2 - https://doi.org/10.1021/ac100060g DB - PRIME DP - Unbound Medicine ER -