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Model membranes prepared with ceramide EOS, cholesterol and free fatty acids form a unique lamellar phase.
Langmuir. 2010 Mar 16; 26(6):4168-75.L

Abstract

The lipid matrix present in the human stratum corneum (the thin, uppermost layer of the skin) is considered to play a crucial role in the skin barrier function. The lipid matrix consists of ceramides, cholesterol, and free fatty acids. The 13 nm lamellar phase present in the lipid matrix of the stratum corneum is very characteristic and plays an important role in the skin barrier function. One subclass of ceramides with a linoleic acid linked to a very long acyl (referred to as EOS) plays a crucial role in the formation of the 13 nm lamellar phase. In this article, we focus on the lipid phase behavior of EOS mixed with cholesterol or with cholesterol and free fatty acids. Our studies reveal that an equimolar ratio of EOS, cholesterol, and free fatty acids forms a lamellar phase with a very long repeat distance of approximately 14.7 nm. This phase exhibits exceptional behavior in that in the thermotropic response the fatty acid chains and the ceramide chains undergo an order-disorder transition in different temperature ranges while part of the hydrocarbon chains of ceramides and fatty acids are mixing in the orthorhombic lattice. On the basis of these observations, a molecular model for the 14.7 nm phase has been proposed in which the lipids are organized in a lamellar phase with three different lipid layers in a symmetric unit cell.

Authors+Show Affiliations

Division of Drug Delivery Technology, P.O. Box 9502, 2300 RA, Leiden/Amsterdam Center for Drug Research, Leiden University, Leiden, The Netherlands.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20121267

Citation

Groen, Daniel, et al. "Model Membranes Prepared With Ceramide EOS, Cholesterol and Free Fatty Acids Form a Unique Lamellar Phase." Langmuir : the ACS Journal of Surfaces and Colloids, vol. 26, no. 6, 2010, pp. 4168-75.
Groen D, Gooris GS, Bouwstra JA. Model membranes prepared with ceramide EOS, cholesterol and free fatty acids form a unique lamellar phase. Langmuir. 2010;26(6):4168-75.
Groen, D., Gooris, G. S., & Bouwstra, J. A. (2010). Model membranes prepared with ceramide EOS, cholesterol and free fatty acids form a unique lamellar phase. Langmuir : the ACS Journal of Surfaces and Colloids, 26(6), 4168-75. https://doi.org/10.1021/la9047038
Groen D, Gooris GS, Bouwstra JA. Model Membranes Prepared With Ceramide EOS, Cholesterol and Free Fatty Acids Form a Unique Lamellar Phase. Langmuir. 2010 Mar 16;26(6):4168-75. PubMed PMID: 20121267.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Model membranes prepared with ceramide EOS, cholesterol and free fatty acids form a unique lamellar phase. AU - Groen,Daniel, AU - Gooris,Gert S, AU - Bouwstra,Joke A, PY - 2010/2/4/entrez PY - 2010/2/4/pubmed PY - 2010/6/2/medline SP - 4168 EP - 75 JF - Langmuir : the ACS journal of surfaces and colloids JO - Langmuir VL - 26 IS - 6 N2 - The lipid matrix present in the human stratum corneum (the thin, uppermost layer of the skin) is considered to play a crucial role in the skin barrier function. The lipid matrix consists of ceramides, cholesterol, and free fatty acids. The 13 nm lamellar phase present in the lipid matrix of the stratum corneum is very characteristic and plays an important role in the skin barrier function. One subclass of ceramides with a linoleic acid linked to a very long acyl (referred to as EOS) plays a crucial role in the formation of the 13 nm lamellar phase. In this article, we focus on the lipid phase behavior of EOS mixed with cholesterol or with cholesterol and free fatty acids. Our studies reveal that an equimolar ratio of EOS, cholesterol, and free fatty acids forms a lamellar phase with a very long repeat distance of approximately 14.7 nm. This phase exhibits exceptional behavior in that in the thermotropic response the fatty acid chains and the ceramide chains undergo an order-disorder transition in different temperature ranges while part of the hydrocarbon chains of ceramides and fatty acids are mixing in the orthorhombic lattice. On the basis of these observations, a molecular model for the 14.7 nm phase has been proposed in which the lipids are organized in a lamellar phase with three different lipid layers in a symmetric unit cell. SN - 1520-5827 UR - https://www.unboundmedicine.com/medline/citation/20121267/Model_membranes_prepared_with_ceramide_EOS_cholesterol_and_free_fatty_acids_form_a_unique_lamellar_phase_ L2 - https://doi.org/10.1021/la9047038 DB - PRIME DP - Unbound Medicine ER -