Off-line comprehensive two-dimensional hydrophilic interaction x reversed phase liquid chromatographic analysis of green tea phenolics.
J Sep Sci. 2010 Mar; 33(6-7):853-63.JS

Abstract

The separation of green tea phenolic compounds by comprehensive 2-D LC (LCxLC) has been studied. An off-line LCxLC system was designed based on hydrophilic interaction chromatography in the first dimension and gradient RP-LC using a C18 column in the second dimension, to separate compounds based on polarity and hydrophobicity, respectively. Green tea was found to contain a complex composition of low molecular weight phenolic compounds, identified by fluorescence, diode-array and negative ESI-MS detection systems. The LCxLC system was characterized by a very high practical peak capacity (>2100, in approximately 22.5 h) due to the low degree of correlation (r(2)<0.2) between the selected separation mechanisms. The proposed methodology demonstrated its suitability for the analysis of various groups of phenolic compounds including proanthocyanidins, phenolic acids, flavonols and flavonol derivatives, all of which cannot be separated in a single analysis by conventional 1-D HPLC methods.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Kalili KM

Department of Chemistry and Polymer Science, Stellenbosch University, Matieland, South Africa.

de Villiers A

No affiliation info available

MeSH

Chromatography, LiquidMass SpectrometryPhenolsTea

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20127915

Citation

Kalili, Kathithileni M., and André de Villiers. "Off-line Comprehensive Two-dimensional Hydrophilic Interaction X Reversed Phase Liquid Chromatographic Analysis of Green Tea Phenolics." Journal of Separation Science, vol. 33, no. 6-7, 2010, pp. 853-63.

Kalili KM, de Villiers A. Off-line comprehensive two-dimensional hydrophilic interaction x reversed phase liquid chromatographic analysis of green tea phenolics. J Sep Sci. 2010;33(6-7):853-63.

Kalili, K. M., & de Villiers, A. (2010). Off-line comprehensive two-dimensional hydrophilic interaction x reversed phase liquid chromatographic analysis of green tea phenolics. Journal of Separation Science, 33(6-7), 853-63. https://doi.org/10.1002/jssc.200900673

Kalili KM, de Villiers A. Off-line Comprehensive Two-dimensional Hydrophilic Interaction X Reversed Phase Liquid Chromatographic Analysis of Green Tea Phenolics. J Sep Sci. 2010;33(6-7):853-63. PubMed PMID: 20127915.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Off-line comprehensive two-dimensional hydrophilic interaction x reversed phase liquid chromatographic analysis of green tea phenolics. AU - Kalili,Kathithileni M, AU - de Villiers,André, PY - 2010/2/4/entrez PY - 2010/2/4/pubmed PY - 2010/9/29/medline SP - 853 EP - 63 JF - Journal of separation science JO - J Sep Sci VL - 33 IS - 6-7 N2 - The separation of green tea phenolic compounds by comprehensive 2-D LC (LCxLC) has been studied. An off-line LCxLC system was designed based on hydrophilic interaction chromatography in the first dimension and gradient RP-LC using a C18 column in the second dimension, to separate compounds based on polarity and hydrophobicity, respectively. Green tea was found to contain a complex composition of low molecular weight phenolic compounds, identified by fluorescence, diode-array and negative ESI-MS detection systems. The LCxLC system was characterized by a very high practical peak capacity (>2100, in approximately 22.5 h) due to the low degree of correlation (r(2)<0.2) between the selected separation mechanisms. The proposed methodology demonstrated its suitability for the analysis of various groups of phenolic compounds including proanthocyanidins, phenolic acids, flavonols and flavonol derivatives, all of which cannot be separated in a single analysis by conventional 1-D HPLC methods. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/20127915/Off_line_comprehensive_two_dimensional_hydrophilic_interaction_x_reversed_phase_liquid_chromatographic_analysis_of_green_tea_phenolics_ L2 - https://doi.org/10.1002/jssc.200900673 DB - PRIME DP - Unbound Medicine ER -

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Off-line comprehensive two-dimensional hydrophilic interaction x reversed phase liquid chromatographic analysis of green tea phenolics.J Sep Sci. 2010 Mar; 33(6-7):853-63.JS