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Tumor necrosis factor-alpha enhances both epithelial-mesenchymal transition and cell contraction induced in A549 human alveolar epithelial cells by transforming growth factor-beta1.
Exp Lung Res. 2010 Feb; 36(1):12-24.EL

Abstract

Recently, epithelial-mesenchymal transition (EMT) has been reported to contribute to tissue fibrosis through enhanced transforming growth factor (TGF)-beta1 signaling. Tumor necrosis factor (TNF)-alpha has also been implicated in tissue fibrosis. Therefore, the authors investigated whether TNF-alpha affected TGF-beta1-induced EMT. Cultured alveolar epithelial cells (A549 cells) were stimulated with TGF-beta1 (5 ng/mL), with/without TNF-alpha (10 ng/mL). TGF-beta1 induced EMT of A549 cells, with loss of E-cadherin and acquisition of vimentin. Combination of TNF-alpha with TGF-beta1 enhanced EMT, causing morphological changes, while quantitative polymerase chain reaction (PCR) showed suppression of E-cadherin mRNA and expression of vimentin mRNA. In addition, the gel contraction method revealed that cells that had undergone EMT acquired cell contractility, which is a feature of mesenchymal cells. Stimulation with TGF-beta1 induced cell contraction, as did TNF-alpha. Moreover, costimulation with TGF-beta1 and TNF-alpha enhanced the cell contraction. Although IFN-gamma suppressed spontaneous cell contraction, it did not suppress cell contraction, which was induced by TGF-beta1. In conclusion, TNF-alpha enhances not only EMT but also cell contraction induced by TGF-beta1. EMT might contribute to tissue fibrosis through induction of cell contraction.

Authors+Show Affiliations

Department of Respiratory Medicine, The University of Tokyo, Tokyo, Japan. YAMAUCHIY-INT@h.u-tokyo.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20128678

Citation

Yamauchi, Yasuhiro, et al. "Tumor Necrosis Factor-alpha Enhances Both Epithelial-mesenchymal Transition and Cell Contraction Induced in A549 Human Alveolar Epithelial Cells By Transforming Growth Factor-beta1." Experimental Lung Research, vol. 36, no. 1, 2010, pp. 12-24.
Yamauchi Y, Kohyama T, Takizawa H, et al. Tumor necrosis factor-alpha enhances both epithelial-mesenchymal transition and cell contraction induced in A549 human alveolar epithelial cells by transforming growth factor-beta1. Exp Lung Res. 2010;36(1):12-24.
Yamauchi, Y., Kohyama, T., Takizawa, H., Kamitani, S., Desaki, M., Takami, K., Kawasaki, S., Kato, J., & Nagase, T. (2010). Tumor necrosis factor-alpha enhances both epithelial-mesenchymal transition and cell contraction induced in A549 human alveolar epithelial cells by transforming growth factor-beta1. Experimental Lung Research, 36(1), 12-24. https://doi.org/10.3109/01902140903042589
Yamauchi Y, et al. Tumor Necrosis Factor-alpha Enhances Both Epithelial-mesenchymal Transition and Cell Contraction Induced in A549 Human Alveolar Epithelial Cells By Transforming Growth Factor-beta1. Exp Lung Res. 2010;36(1):12-24. PubMed PMID: 20128678.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tumor necrosis factor-alpha enhances both epithelial-mesenchymal transition and cell contraction induced in A549 human alveolar epithelial cells by transforming growth factor-beta1. AU - Yamauchi,Yasuhiro, AU - Kohyama,Tadashi, AU - Takizawa,Hajime, AU - Kamitani,Sumiko, AU - Desaki,Masashi, AU - Takami,Kazutaka, AU - Kawasaki,Shin, AU - Kato,Jun, AU - Nagase,Takahide, PY - 2010/2/5/entrez PY - 2010/2/5/pubmed PY - 2010/5/25/medline SP - 12 EP - 24 JF - Experimental lung research JO - Exp Lung Res VL - 36 IS - 1 N2 - Recently, epithelial-mesenchymal transition (EMT) has been reported to contribute to tissue fibrosis through enhanced transforming growth factor (TGF)-beta1 signaling. Tumor necrosis factor (TNF)-alpha has also been implicated in tissue fibrosis. Therefore, the authors investigated whether TNF-alpha affected TGF-beta1-induced EMT. Cultured alveolar epithelial cells (A549 cells) were stimulated with TGF-beta1 (5 ng/mL), with/without TNF-alpha (10 ng/mL). TGF-beta1 induced EMT of A549 cells, with loss of E-cadherin and acquisition of vimentin. Combination of TNF-alpha with TGF-beta1 enhanced EMT, causing morphological changes, while quantitative polymerase chain reaction (PCR) showed suppression of E-cadherin mRNA and expression of vimentin mRNA. In addition, the gel contraction method revealed that cells that had undergone EMT acquired cell contractility, which is a feature of mesenchymal cells. Stimulation with TGF-beta1 induced cell contraction, as did TNF-alpha. Moreover, costimulation with TGF-beta1 and TNF-alpha enhanced the cell contraction. Although IFN-gamma suppressed spontaneous cell contraction, it did not suppress cell contraction, which was induced by TGF-beta1. In conclusion, TNF-alpha enhances not only EMT but also cell contraction induced by TGF-beta1. EMT might contribute to tissue fibrosis through induction of cell contraction. SN - 1521-0499 UR - https://www.unboundmedicine.com/medline/citation/20128678/Tumor_necrosis_factor_alpha_enhances_both_epithelial_mesenchymal_transition_and_cell_contraction_induced_in_A549_human_alveolar_epithelial_cells_by_transforming_growth_factor_beta1_ L2 - https://www.tandfonline.com/doi/full/10.3109/01902140903042589 DB - PRIME DP - Unbound Medicine ER -