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Pattern of serum cytokine expression and T-cell subsets in sickle cell disease patients in vaso-occlusive crisis.
Clin Vaccine Immunol. 2010 Apr; 17(4):602-8.CV

Abstract

The pathogenesis of sickle vaso-occlusive crisis (VOC) in sickle cell disease (SCD) patients involves the accumulation of rigid sickle cells and the stimulation of an ongoing inflammatory response, as well as the stress of infections. The immune response, via cytokine imbalances and deregulated T-cell subsets, also has been proposed to contribute to the development of VOC. In this study, a panel of high-sensitivity cytokine kits was used to investigate cytokines in the sera of SCD patients in VOC. The results were compared primarily with those for stable SCD patients and secondarily with those for normal healthy people who served as controls. The cytokines studied included interleukin-2 (IL-2), IL-4, and IL-10. Lymphocyte subsets of patients with VOC were also studied and were compared with those of both control groups (20 stable patients without crisis [SCD group] and 20 normal healthy controls [NHC]). The VOC group was notable for remarkably elevated levels of IL-4, among the three cytokines tested, compared with those for the SCD and NHC groups. Patients with VOC also differed from stable SCD patients and NHC by having notably lower IL-10 levels, as well as the lowest ratio of CD4(+) to CD8(+) T cells (0.7). The patterns of the proinflammatory cytokine IL-2 did not differ between VOC and stable SCD patients, but NHC had significantly lower IL-2 levels than both the VOC and SCD groups. Our results demonstrate coexisting levels, both high and low, of TH1- and TH2-type cytokines, as well as diminished levels of T-cell subsets in VOC. These results are discussed in an effort to better understand the importance of the immune system profile in the pathogenesis of sickle cell VOC. Since the possibility that a cytokine imbalance is implicated in the pathogenesis of sickle cell crisis has been raised, our results should prompt further investigation of the host immune response in terms of TH1 and TH2 balance in sickle cell crisis.

Authors+Show Affiliations

Immunology Unit, Department of Medicine, ABUTH, Zaria, Nigeria. bolamusa2002@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20130127

Citation

Musa, Bolanle O P., et al. "Pattern of Serum Cytokine Expression and T-cell Subsets in Sickle Cell Disease Patients in Vaso-occlusive Crisis." Clinical and Vaccine Immunology : CVI, vol. 17, no. 4, 2010, pp. 602-8.
Musa BO, Onyemelukwe GC, Hambolu JO, et al. Pattern of serum cytokine expression and T-cell subsets in sickle cell disease patients in vaso-occlusive crisis. Clin Vaccine Immunol. 2010;17(4):602-8.
Musa, B. O., Onyemelukwe, G. C., Hambolu, J. O., Mamman, A. I., & Isa, A. H. (2010). Pattern of serum cytokine expression and T-cell subsets in sickle cell disease patients in vaso-occlusive crisis. Clinical and Vaccine Immunology : CVI, 17(4), 602-8. https://doi.org/10.1128/CVI.00145-09
Musa BO, et al. Pattern of Serum Cytokine Expression and T-cell Subsets in Sickle Cell Disease Patients in Vaso-occlusive Crisis. Clin Vaccine Immunol. 2010;17(4):602-8. PubMed PMID: 20130127.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pattern of serum cytokine expression and T-cell subsets in sickle cell disease patients in vaso-occlusive crisis. AU - Musa,Bolanle O P, AU - Onyemelukwe,Geoffrey C, AU - Hambolu,Joseph O, AU - Mamman,Aisha I, AU - Isa,Albarka H, Y1 - 2010/02/03/ PY - 2010/2/5/entrez PY - 2010/2/5/pubmed PY - 2010/6/16/medline SP - 602 EP - 8 JF - Clinical and vaccine immunology : CVI JO - Clin. Vaccine Immunol. VL - 17 IS - 4 N2 - The pathogenesis of sickle vaso-occlusive crisis (VOC) in sickle cell disease (SCD) patients involves the accumulation of rigid sickle cells and the stimulation of an ongoing inflammatory response, as well as the stress of infections. The immune response, via cytokine imbalances and deregulated T-cell subsets, also has been proposed to contribute to the development of VOC. In this study, a panel of high-sensitivity cytokine kits was used to investigate cytokines in the sera of SCD patients in VOC. The results were compared primarily with those for stable SCD patients and secondarily with those for normal healthy people who served as controls. The cytokines studied included interleukin-2 (IL-2), IL-4, and IL-10. Lymphocyte subsets of patients with VOC were also studied and were compared with those of both control groups (20 stable patients without crisis [SCD group] and 20 normal healthy controls [NHC]). The VOC group was notable for remarkably elevated levels of IL-4, among the three cytokines tested, compared with those for the SCD and NHC groups. Patients with VOC also differed from stable SCD patients and NHC by having notably lower IL-10 levels, as well as the lowest ratio of CD4(+) to CD8(+) T cells (0.7). The patterns of the proinflammatory cytokine IL-2 did not differ between VOC and stable SCD patients, but NHC had significantly lower IL-2 levels than both the VOC and SCD groups. Our results demonstrate coexisting levels, both high and low, of TH1- and TH2-type cytokines, as well as diminished levels of T-cell subsets in VOC. These results are discussed in an effort to better understand the importance of the immune system profile in the pathogenesis of sickle cell VOC. Since the possibility that a cytokine imbalance is implicated in the pathogenesis of sickle cell crisis has been raised, our results should prompt further investigation of the host immune response in terms of TH1 and TH2 balance in sickle cell crisis. SN - 1556-679X UR - https://www.unboundmedicine.com/medline/citation/20130127/Pattern_of_serum_cytokine_expression_and_T_cell_subsets_in_sickle_cell_disease_patients_in_vaso_occlusive_crisis_ L2 - http://cvi.asm.org/cgi/pmidlookup?view=long&pmid=20130127 DB - PRIME DP - Unbound Medicine ER -