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Effect of vitamin A treatment on superoxide dismutase-deficient yeast strains.
Arch Microbiol. 2010 Mar; 192(3):221-8.AM

Abstract

Vitamin A (Vit A) is widely suggested to be protective against oxidative stress. However, different studies have been demonstrated the pro-oxidant effects of retinoids in several experimental models. In this work, we used the yeast Saccharomyces cerevisiae as a model organism to study the Vit A effects on superoxide dismutase (SOD)-deficient yeast strains. We report here that Vit A (10, 20 and 40 mg/ml) decreases the survival of exponentially growing yeast cells, especially in strains deficient in CuZnSOD (sod1Delta) and CuZnSOD/MnSOD (sod1Deltasod2Delta). We also observed the protective effect of vitamin E against the Vit A-induced toxicity. Possible adaptation effects induced by sub-lethal oxidative stress were monitored by pre-, co- and post-treatment with the oxidative agent paraquat. The enzymatic activities of catalase (CAT) and glutathione peroxidase (GPx), and the total glutathione content were determined after Vit A treatment. Our results showed that CuZnSOD represents an important defence against Vit A-generated oxidative damage. In SOD-deficient strains, the main defence against Vit A-produced reactive oxygen species (ROS) is GPx. However, the induction of GPx activity is not sufficient to prevent the Vit A-induced cell death in these mutants in exponential phase growth.

Authors+Show Affiliations

Departamento de Biofísica, IB/UFRGS, Porto Alegre, RS, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20131044

Citation

Roehrs, Rafael, et al. "Effect of Vitamin a Treatment On Superoxide Dismutase-deficient Yeast Strains." Archives of Microbiology, vol. 192, no. 3, 2010, pp. 221-8.
Roehrs R, Freitas DR, Masuda A, et al. Effect of vitamin A treatment on superoxide dismutase-deficient yeast strains. Arch Microbiol. 2010;192(3):221-8.
Roehrs, R., Freitas, D. R., Masuda, A., Henriques, J. A., Guecheva, T. N., Ramos, A. L., & Saffi, J. (2010). Effect of vitamin A treatment on superoxide dismutase-deficient yeast strains. Archives of Microbiology, 192(3), 221-8. https://doi.org/10.1007/s00203-010-0551-2
Roehrs R, et al. Effect of Vitamin a Treatment On Superoxide Dismutase-deficient Yeast Strains. Arch Microbiol. 2010;192(3):221-8. PubMed PMID: 20131044.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of vitamin A treatment on superoxide dismutase-deficient yeast strains. AU - Roehrs,Rafael, AU - Freitas,Daniela R J, AU - Masuda,Aoi, AU - Henriques,João A P, AU - Guecheva,Temenouga N, AU - Ramos,Ana-Ligia L P, AU - Saffi,Jenifer, Y1 - 2010/02/04/ PY - 2009/11/20/received PY - 2010/01/12/accepted PY - 2010/01/11/revised PY - 2010/2/5/entrez PY - 2010/2/5/pubmed PY - 2010/4/3/medline SP - 221 EP - 8 JF - Archives of microbiology JO - Arch Microbiol VL - 192 IS - 3 N2 - Vitamin A (Vit A) is widely suggested to be protective against oxidative stress. However, different studies have been demonstrated the pro-oxidant effects of retinoids in several experimental models. In this work, we used the yeast Saccharomyces cerevisiae as a model organism to study the Vit A effects on superoxide dismutase (SOD)-deficient yeast strains. We report here that Vit A (10, 20 and 40 mg/ml) decreases the survival of exponentially growing yeast cells, especially in strains deficient in CuZnSOD (sod1Delta) and CuZnSOD/MnSOD (sod1Deltasod2Delta). We also observed the protective effect of vitamin E against the Vit A-induced toxicity. Possible adaptation effects induced by sub-lethal oxidative stress were monitored by pre-, co- and post-treatment with the oxidative agent paraquat. The enzymatic activities of catalase (CAT) and glutathione peroxidase (GPx), and the total glutathione content were determined after Vit A treatment. Our results showed that CuZnSOD represents an important defence against Vit A-generated oxidative damage. In SOD-deficient strains, the main defence against Vit A-produced reactive oxygen species (ROS) is GPx. However, the induction of GPx activity is not sufficient to prevent the Vit A-induced cell death in these mutants in exponential phase growth. SN - 1432-072X UR - https://www.unboundmedicine.com/medline/citation/20131044/Effect_of_vitamin_A_treatment_on_superoxide_dismutase_deficient_yeast_strains_ L2 - https://dx.doi.org/10.1007/s00203-010-0551-2 DB - PRIME DP - Unbound Medicine ER -