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Hyul-Tong-Ryung suppresses PMA-induced MMP-9 expression by inhibiting AP-1-mediated gene expression via ERK 1/2 signaling pathway in MCF-7 human breast cancer cells.
Immunopharmacol Immunotoxicol. 2010 Dec; 32(4):600-6.II

Abstract

Our previous study has demonstrated that the methanol extract of Hyul-Tong-Ryung (HM) specifically suppresses the phorbol 12-myristate 13-acetate (PMA)-induced matrix metalloproteinase-9 (MMP-9) production through the inhibition of MMP-9 mRNA expression in MCF-7 human breast carcinoma cells. However, the molecular mechanisms involved in transcriptional suppression of MMP-9 by HM in PMA-induced MCF-7 cells are not known. In this study, we aimed to elucidate the molecular mechanisms involved in the inhibition of MMP-9 expression by HM in PMA-induced MCF-7 cells. The results of promoter assay and EMSA showed that HM specifically inhibits MMP-9 gene expression by blocking PMA-stimulated activation of activator protein-1 (AP-1). In addition, PMA-stimulated phosphorylation of extracellular signal regulated kinase 1/2 (ERK 1/2) was suppressed by HM treatment, whereas the phosphorylation of either c-Jun N-terminal kinase (JNK) or p38 mitogen-activated protein kinase (MAPK) was not affected. HM could inhibit the PMA-induced MMP-9 expression through suppression of the transcriptional activity of MMP-9 gene in MCF-7 cells. These results indicate that HM inhibits PMA-induced MMP-9 expression by blocking the activation of activator protein-1 (AP-1) via extracellular signal regulated kinase 1/2 (ERK 1/2) signaling pathway.

Authors+Show Affiliations

College of Natural Resources and Life Science, BK21 Center for Silver-Bio Industrialization, Dong-A University, Busan, South Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20131957

Citation

Kim, Kyoung-Sook, et al. "Hyul-Tong-Ryung Suppresses PMA-induced MMP-9 Expression By Inhibiting AP-1-mediated Gene Expression Via ERK 1/2 Signaling Pathway in MCF-7 Human Breast Cancer Cells." Immunopharmacology and Immunotoxicology, vol. 32, no. 4, 2010, pp. 600-6.
Kim KS, Yao L, Lee YC, et al. Hyul-Tong-Ryung suppresses PMA-induced MMP-9 expression by inhibiting AP-1-mediated gene expression via ERK 1/2 signaling pathway in MCF-7 human breast cancer cells. Immunopharmacol Immunotoxicol. 2010;32(4):600-6.
Kim, K. S., Yao, L., Lee, Y. C., Chung, E., Kim, K. M., Kwak, Y. J., Kim, S. J., Cui, Z., & Lee, J. H. (2010). Hyul-Tong-Ryung suppresses PMA-induced MMP-9 expression by inhibiting AP-1-mediated gene expression via ERK 1/2 signaling pathway in MCF-7 human breast cancer cells. Immunopharmacology and Immunotoxicology, 32(4), 600-6. https://doi.org/10.3109/08923971003610817
Kim KS, et al. Hyul-Tong-Ryung Suppresses PMA-induced MMP-9 Expression By Inhibiting AP-1-mediated Gene Expression Via ERK 1/2 Signaling Pathway in MCF-7 Human Breast Cancer Cells. Immunopharmacol Immunotoxicol. 2010;32(4):600-6. PubMed PMID: 20131957.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hyul-Tong-Ryung suppresses PMA-induced MMP-9 expression by inhibiting AP-1-mediated gene expression via ERK 1/2 signaling pathway in MCF-7 human breast cancer cells. AU - Kim,Kyoung-Sook, AU - Yao,Lan, AU - Lee,Young-Choon, AU - Chung,Eunsook, AU - Kim,Kyung-Mi, AU - Kwak,Yeon-Joo, AU - Kim,Seok-Jo, AU - Cui,Zheng, AU - Lee,Jai-Heon, Y1 - 2010/02/04/ PY - 2010/2/6/entrez PY - 2010/2/6/pubmed PY - 2011/3/10/medline SP - 600 EP - 6 JF - Immunopharmacology and immunotoxicology JO - Immunopharmacol Immunotoxicol VL - 32 IS - 4 N2 - Our previous study has demonstrated that the methanol extract of Hyul-Tong-Ryung (HM) specifically suppresses the phorbol 12-myristate 13-acetate (PMA)-induced matrix metalloproteinase-9 (MMP-9) production through the inhibition of MMP-9 mRNA expression in MCF-7 human breast carcinoma cells. However, the molecular mechanisms involved in transcriptional suppression of MMP-9 by HM in PMA-induced MCF-7 cells are not known. In this study, we aimed to elucidate the molecular mechanisms involved in the inhibition of MMP-9 expression by HM in PMA-induced MCF-7 cells. The results of promoter assay and EMSA showed that HM specifically inhibits MMP-9 gene expression by blocking PMA-stimulated activation of activator protein-1 (AP-1). In addition, PMA-stimulated phosphorylation of extracellular signal regulated kinase 1/2 (ERK 1/2) was suppressed by HM treatment, whereas the phosphorylation of either c-Jun N-terminal kinase (JNK) or p38 mitogen-activated protein kinase (MAPK) was not affected. HM could inhibit the PMA-induced MMP-9 expression through suppression of the transcriptional activity of MMP-9 gene in MCF-7 cells. These results indicate that HM inhibits PMA-induced MMP-9 expression by blocking the activation of activator protein-1 (AP-1) via extracellular signal regulated kinase 1/2 (ERK 1/2) signaling pathway. SN - 1532-2513 UR - https://www.unboundmedicine.com/medline/citation/20131957/Hyul_Tong_Ryung_suppresses_PMA_induced_MMP_9_expression_by_inhibiting_AP_1_mediated_gene_expression_via_ERK_1/2_signaling_pathway_in_MCF_7_human_breast_cancer_cells_ L2 - https://www.tandfonline.com/doi/full/10.3109/08923971003610817 DB - PRIME DP - Unbound Medicine ER -