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Assessment of RNA amplification by multiplex RT-PCR and IgM detection by indirect and capture ELISAs for the diagnosis of measles and rubella.
APMIS. 2010 Mar; 118(3):203-9.A

Abstract

The aim of the study was to compare RNA amplification using multiplex RT-PCR and IgM detection by means of indirect and capture ELISAs for the diagnosis of measles and rubella. A total of 229 cases of maculopapular rash with serum and throat swab samples were included. Specific serological IgM to measles and rubella was determined by Enzygnost (Siemens) and Platelia (Bio-Rad). Both viruses were researched using multiplex RT-PCR performed on throat samples. Criteria for inclusion of measles or rubella cases were a positive RT-PCR result for one virus and negative for the other; and/or a positive IgM result for one virus by both ELISAs and negative RT-PCR for the other virus. A total of 74 cases were classified as measles and 54 as rubella. In measles, sensitivity and specificity were 93.2% and 100% for RT-PCR, 97.3% and 98.1% for Enzygnost, and 90.5% and 95.5% for Platelia. For rubella, these values were 42.6% and 100% for RT-PCR, 100% and 97.1% for Enzygnost, and 94.4% and 98.3% for Platelia. Enzygnost and Platelia are useful techniques for detecting IgM against measles and rubella. RNA amplification by RT-PCR was both sensitive and specific for the diagnosis of measles; however, for rubella, the sensitivity of this technique must be improved.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Sanz JC
Laboratorio Regional de Salud Pública de la Comunidad de Madrid, Madrid, Spain. juan.sanz@salud.madrid.org
Mosquera M
No affiliation info available
Ramos B
No affiliation info available
Ramírez R
No affiliation info available
de Ory F
No affiliation info available
Echevarria JE
No affiliation info available

MeSH

AdolescentAdultAntibodies, ViralChildChild, PreschoolEnzyme-Linked Immunosorbent AssayFemaleHumansImmunoglobulin MInfantMaleMeaslesMeasles virusRNA, ViralReverse Transcriptase Polymerase Chain ReactionRubellaRubella virus

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20132186

Citation

Sanz, Juan Carlos, et al. "Assessment of RNA Amplification By Multiplex RT-PCR and IgM Detection By Indirect and Capture ELISAs for the Diagnosis of Measles and Rubella." APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica, vol. 118, no. 3, 2010, pp. 203-9.
Sanz JC, Mosquera M, Ramos B, et al. Assessment of RNA amplification by multiplex RT-PCR and IgM detection by indirect and capture ELISAs for the diagnosis of measles and rubella. APMIS. 2010;118(3):203-9.
Sanz, J. C., Mosquera, M., Ramos, B., Ramírez, R., de Ory, F., & Echevarria, J. E. (2010). Assessment of RNA amplification by multiplex RT-PCR and IgM detection by indirect and capture ELISAs for the diagnosis of measles and rubella. APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica, 118(3), 203-9. https://doi.org/10.1111/j.1600-0463.2009.02581.x
Sanz JC, et al. Assessment of RNA Amplification By Multiplex RT-PCR and IgM Detection By Indirect and Capture ELISAs for the Diagnosis of Measles and Rubella. APMIS. 2010;118(3):203-9. PubMed PMID: 20132186.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Assessment of RNA amplification by multiplex RT-PCR and IgM detection by indirect and capture ELISAs for the diagnosis of measles and rubella. AU - Sanz,Juan Carlos, AU - Mosquera,Mar, AU - Ramos,Belén, AU - Ramírez,Rosa, AU - de Ory,Fernando, AU - Echevarria,Juan Emilio, PY - 2010/2/6/entrez PY - 2010/2/6/pubmed PY - 2010/3/3/medline SP - 203 EP - 9 JF - APMIS : acta pathologica, microbiologica, et immunologica Scandinavica JO - APMIS VL - 118 IS - 3 N2 - The aim of the study was to compare RNA amplification using multiplex RT-PCR and IgM detection by means of indirect and capture ELISAs for the diagnosis of measles and rubella. A total of 229 cases of maculopapular rash with serum and throat swab samples were included. Specific serological IgM to measles and rubella was determined by Enzygnost (Siemens) and Platelia (Bio-Rad). Both viruses were researched using multiplex RT-PCR performed on throat samples. Criteria for inclusion of measles or rubella cases were a positive RT-PCR result for one virus and negative for the other; and/or a positive IgM result for one virus by both ELISAs and negative RT-PCR for the other virus. A total of 74 cases were classified as measles and 54 as rubella. In measles, sensitivity and specificity were 93.2% and 100% for RT-PCR, 97.3% and 98.1% for Enzygnost, and 90.5% and 95.5% for Platelia. For rubella, these values were 42.6% and 100% for RT-PCR, 100% and 97.1% for Enzygnost, and 94.4% and 98.3% for Platelia. Enzygnost and Platelia are useful techniques for detecting IgM against measles and rubella. RNA amplification by RT-PCR was both sensitive and specific for the diagnosis of measles; however, for rubella, the sensitivity of this technique must be improved. SN - 1600-0463 UR - https://www.unboundmedicine.com/medline/citation/20132186/Assessment_of_RNA_amplification_by_multiplex_RT_PCR_and_IgM_detection_by_indirect_and_capture_ELISAs_for_the_diagnosis_of_measles_and_rubella_ DB - PRIME DP - Unbound Medicine ER -