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Leukotriene B4, 8-iso-prostaglandin F2 alpha, and pH in exhaled breath condensate from asymptomatic smokers.
J Physiol Pharmacol. 2009 Nov; 60 Suppl 5:57-60.JP

Abstract

Long-term cigarette smoking is the major etiological factor for the development of chronic obstructive pulmonary disease (COPD). Airway inflammation and oxidative stress are implicated in the pathogenesis. Biomarkers reflecting these responses could be analyzed in exhaled breath condensate (EBC). Recently, it became obvious that mediator concentrations in EBC could be influenced by age of the subjects, equipment, sampling properties, and the analytical assays applied. In the present study we evaluated 8 smokers (20-56 yr) and 16 non-smoking adults (18-60 yr) with normal spirometry and no episode of airway infection during 6 weeks prior to the study. EBC samples were obtained with the commercial device ECoScreen2 at a controlled temperature of -20 degrees C. Leukotriene B(4) (LTB(4), marker of inflammation), 8-iso-prostaglandin F(2 alpha) (8-iso-PGF(2 alpha)/8-isoprostane, oxidative stress) concentrations, and pH were measured. With 10 min of tidal breathing, a lower EBC volume was collected in smokers (median 1.22 ml; interquantil range 1.06-1.74 ml) than in non-smokers (1.6 ml; 1.16-2.21 ml; P=0.06). Significant differences were found in pH in smokers compared with non-smokers (7.14 (5.70-7.43) vs. 7.59 (7.28-7.73); P<0.01). No significant differences were observed in EBC concentrations of LTB(4) or 8-iso-PGF(2 alpha). The study demonstrates that acidopnea is detectable in otherwise asymptomatic smokers and might precede changes in the level of arachidonic acid metabolites. For pH is considered to be the most validated marker determined in EBC samples, it may be useful for screening asymptomatic individuals for smoking-induced early airway damage.

Authors+Show Affiliations

BGFA, Research Institute of Occupational Medicine, German Social Accident Insurance, Ruhr-University Bochum, Germany. hoffmeyer@bgfa.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

20134040

Citation

Hoffmeyer, F, et al. "Leukotriene B4, 8-iso-prostaglandin F2 Alpha, and pH in Exhaled Breath Condensate From Asymptomatic Smokers." Journal of Physiology and Pharmacology : an Official Journal of the Polish Physiological Society, vol. 60 Suppl 5, 2009, pp. 57-60.
Hoffmeyer F, Harth V, Bunger J, et al. Leukotriene B4, 8-iso-prostaglandin F2 alpha, and pH in exhaled breath condensate from asymptomatic smokers. J Physiol Pharmacol. 2009;60 Suppl 5:57-60.
Hoffmeyer, F., Harth, V., Bunger, J., Bruning, T., & Raulf-Heimsoth, M. (2009). Leukotriene B4, 8-iso-prostaglandin F2 alpha, and pH in exhaled breath condensate from asymptomatic smokers. Journal of Physiology and Pharmacology : an Official Journal of the Polish Physiological Society, 60 Suppl 5, 57-60.
Hoffmeyer F, et al. Leukotriene B4, 8-iso-prostaglandin F2 Alpha, and pH in Exhaled Breath Condensate From Asymptomatic Smokers. J Physiol Pharmacol. 2009;60 Suppl 5:57-60. PubMed PMID: 20134040.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Leukotriene B4, 8-iso-prostaglandin F2 alpha, and pH in exhaled breath condensate from asymptomatic smokers. AU - Hoffmeyer,F, AU - Harth,V, AU - Bunger,J, AU - Bruning,T, AU - Raulf-Heimsoth,M, PY - 2009/07/27/received PY - 2009/10/15/accepted PY - 2010/2/6/entrez PY - 2010/2/27/pubmed PY - 2010/9/21/medline SP - 57 EP - 60 JF - Journal of physiology and pharmacology : an official journal of the Polish Physiological Society JO - J Physiol Pharmacol VL - 60 Suppl 5 N2 - Long-term cigarette smoking is the major etiological factor for the development of chronic obstructive pulmonary disease (COPD). Airway inflammation and oxidative stress are implicated in the pathogenesis. Biomarkers reflecting these responses could be analyzed in exhaled breath condensate (EBC). Recently, it became obvious that mediator concentrations in EBC could be influenced by age of the subjects, equipment, sampling properties, and the analytical assays applied. In the present study we evaluated 8 smokers (20-56 yr) and 16 non-smoking adults (18-60 yr) with normal spirometry and no episode of airway infection during 6 weeks prior to the study. EBC samples were obtained with the commercial device ECoScreen2 at a controlled temperature of -20 degrees C. Leukotriene B(4) (LTB(4), marker of inflammation), 8-iso-prostaglandin F(2 alpha) (8-iso-PGF(2 alpha)/8-isoprostane, oxidative stress) concentrations, and pH were measured. With 10 min of tidal breathing, a lower EBC volume was collected in smokers (median 1.22 ml; interquantil range 1.06-1.74 ml) than in non-smokers (1.6 ml; 1.16-2.21 ml; P=0.06). Significant differences were found in pH in smokers compared with non-smokers (7.14 (5.70-7.43) vs. 7.59 (7.28-7.73); P<0.01). No significant differences were observed in EBC concentrations of LTB(4) or 8-iso-PGF(2 alpha). The study demonstrates that acidopnea is detectable in otherwise asymptomatic smokers and might precede changes in the level of arachidonic acid metabolites. For pH is considered to be the most validated marker determined in EBC samples, it may be useful for screening asymptomatic individuals for smoking-induced early airway damage. SN - 1899-1505 UR - https://www.unboundmedicine.com/medline/citation/20134040/Leukotriene_B4_8_iso_prostaglandin_F2_alpha_and_pH_in_exhaled_breath_condensate_from_asymptomatic_smokers_ L2 - https://medlineplus.gov/smokingandyouth.html DB - PRIME DP - Unbound Medicine ER -