Tags

Type your tag names separated by a space and hit enter

Screening of herbal extracts against multi-drug resistant Acinetobacter baumannii.
Phytother Res. 2010 Aug; 24(8):1202-6.PR

Abstract

Antibiotic resistance is increasing resulting in a decreasing number of fully active antimicrobial agents available to treat infections with multi-drug resistant (MDR) bacteria. Herbal medicines may offer alternative treatment options. A direct inoculation method simulating the standard disc diffusion assay was developed to determine in vitro antimicrobial activity of sixty herbal extracts against MDR-Acinetobacter baumannii (A. baumannii). Eighteen herbal extracts inhibited MDR-A. baumannii on agar plates, although the magnitude and quality of bacterial inhibition differed considerably among the antibacterial herbal extracts. Next, minimal inhibitory concentration (MIC) of these antibacterial herbal extracts was calculated using a broth microdilution assay. For most herbal extracts, the larger the zone of inhibition on agar plates, the lower the MIC. In general, hetero-resistance on agar plates correlated with higher MIC. The skip well phenomenon was seen with two herbal extracts. In conclusion, 30% of the screened herbal extracts demonstrated in vitro antibacterial activity against MDR-A. baumannii using similar rigorous testing methods as those commonly employed for assessing antimicrobial activity of synthetic antibacterial agents. Characterization of a specific compound conferring this antibacterial activity of the herbal extracts may help to identify novel antimicrobial agents active against highly resistant bacteria.

Authors+Show Affiliations

Cedars-Sinai Medical Center, Los Angeles, California, USA. yoko.miyasaki@cshs.orgNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20135638

Citation

Miyasaki, Yoko, et al. "Screening of Herbal Extracts Against Multi-drug Resistant Acinetobacter Baumannii." Phytotherapy Research : PTR, vol. 24, no. 8, 2010, pp. 1202-6.
Miyasaki Y, Nichols WS, Morgan MA, et al. Screening of herbal extracts against multi-drug resistant Acinetobacter baumannii. Phytother Res. 2010;24(8):1202-6.
Miyasaki, Y., Nichols, W. S., Morgan, M. A., Kwan, J. A., Van Benschoten, M. M., Kittell, P. E., & Hardy, W. D. (2010). Screening of herbal extracts against multi-drug resistant Acinetobacter baumannii. Phytotherapy Research : PTR, 24(8), 1202-6. https://doi.org/10.1002/ptr.3113
Miyasaki Y, et al. Screening of Herbal Extracts Against Multi-drug Resistant Acinetobacter Baumannii. Phytother Res. 2010;24(8):1202-6. PubMed PMID: 20135638.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Screening of herbal extracts against multi-drug resistant Acinetobacter baumannii. AU - Miyasaki,Yoko, AU - Nichols,W Stephen, AU - Morgan,Margie A, AU - Kwan,Jamie A, AU - Van Benschoten,M M, AU - Kittell,Patricia Emmett, AU - Hardy,W David, PY - 2010/2/6/entrez PY - 2010/2/6/pubmed PY - 2010/9/24/medline SP - 1202 EP - 6 JF - Phytotherapy research : PTR JO - Phytother Res VL - 24 IS - 8 N2 - Antibiotic resistance is increasing resulting in a decreasing number of fully active antimicrobial agents available to treat infections with multi-drug resistant (MDR) bacteria. Herbal medicines may offer alternative treatment options. A direct inoculation method simulating the standard disc diffusion assay was developed to determine in vitro antimicrobial activity of sixty herbal extracts against MDR-Acinetobacter baumannii (A. baumannii). Eighteen herbal extracts inhibited MDR-A. baumannii on agar plates, although the magnitude and quality of bacterial inhibition differed considerably among the antibacterial herbal extracts. Next, minimal inhibitory concentration (MIC) of these antibacterial herbal extracts was calculated using a broth microdilution assay. For most herbal extracts, the larger the zone of inhibition on agar plates, the lower the MIC. In general, hetero-resistance on agar plates correlated with higher MIC. The skip well phenomenon was seen with two herbal extracts. In conclusion, 30% of the screened herbal extracts demonstrated in vitro antibacterial activity against MDR-A. baumannii using similar rigorous testing methods as those commonly employed for assessing antimicrobial activity of synthetic antibacterial agents. Characterization of a specific compound conferring this antibacterial activity of the herbal extracts may help to identify novel antimicrobial agents active against highly resistant bacteria. SN - 1099-1573 UR - https://www.unboundmedicine.com/medline/citation/20135638/Screening_of_herbal_extracts_against_multi_drug_resistant_Acinetobacter_baumannii_ L2 - https://doi.org/10.1002/ptr.3113 DB - PRIME DP - Unbound Medicine ER -